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Location of chlorogenic acid biosynthesis pathway and poliphenol oxidase genes in a new interspecific anchored linkage map of eggplant

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Location of chlorogenic acid biosynthesis pathway and poliphenol oxidase genes in a new interspecific anchored linkage map of eggplant

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dc.contributor.author Gramazio, Pietro es_ES
dc.contributor.author Prohens Tomás, Jaime es_ES
dc.contributor.author Plazas Ávila, María de la O es_ES
dc.contributor.author Andújar Pérez, Isabel es_ES
dc.contributor.author Herraiz García, Francisco Javier es_ES
dc.contributor.author Castillo Lorenzo, Elena es_ES
dc.contributor.author Knapp, S. es_ES
dc.contributor.author Meyer, R.M. es_ES
dc.contributor.author Vilanova Navarro, Santiago es_ES
dc.date.accessioned 2016-04-28T06:28:36Z
dc.date.available 2016-04-28T06:28:36Z
dc.date.issued 2014-12-10
dc.identifier.issn 1471-2229
dc.identifier.uri http://hdl.handle.net/10251/63082
dc.description.abstract Background Eggplant is a powerful source of polyphenols which seems to play a key role in the prevention of several human diseases, such as cancer and diabetes. Chlorogenic acid is the polyphenol most present in eggplant, comprising between the 70% and 90% of the total polyphenol content. Introduction of the high chlorogenic acid content of wild relatives, such as S. incanum, into eggplant varieties will be of great interest. A potential side effect of the increased level polyphenols could be a decrease on apparent quality due to browning caused by the polyphenol oxidase enzymes mediated oxidation of polyphenols. We report the development of a new interspecific S. melongena × S. incanum linkage map based on a first backcross generation (BC1) towards the cultivated S. melongena as a tool for introgressing S. incanum alleles involved in the biosynthesis of chlorogenic acid in the genetic background of S. melongena. Results The interspecific genetic linkage map of eggplant developed in this work anchor the most informative previously published genetic maps of eggplant using common markers. The 91 BC1 plants of the mapping population were genotyped with 42 COSII, 99 SSRs, 88 AFLPs, 9 CAPS, 4 SNPs and one morphological polymorphic markers. Segregation marker data resulted in a map encompassing 1085 cM distributed in 12 linkage groups. Based on the syntheny with tomato, the candidate genes involved in the core chlorogenic acid synthesis pathway in eggplant (PAL, C4H, 4CL, HCT, C3′H, HQT) as well as five polyphenol oxidase (PPO1, PPO2, PPO3, PPO4, PPO5) were mapped. Except for 4CL and HCT chlorogenic acid genes were not linked. On the contrary, all PPO genes clustered together. Candidate genes important in domestication such as fruit shape (OVATE, SISUN1) and prickliness were also located. Conclusions The achievements in location of candidate genes will allow the search of favorable alleles employing marker-assisted selection in order to develop new varieties with higher chlorogenic content alongside a lower polyphenol oxidase activity. This will result into an enhanced product showing a lower fruit flesh browning with improved human health properties. es_ES
dc.description.sponsorship This research has been partially funded by Ministerio de Economa y Competitividad and FEDER (grant AGL2012-34213). Pietro Gramazio is grateful to Universitat Politcnica de Valncia for a pre-doctoral grant. en_EN
dc.language Inglés es_ES
dc.publisher BioMed Central es_ES
dc.relation.ispartof BMC Plant Biology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Chlorogenic acid es_ES
dc.subject Genetic map es_ES
dc.subject Polyphenol oxidases es_ES
dc.subject Solanum incanum es_ES
dc.subject Solanum melongena es_ES
dc.subject Synteny es_ES
dc.subject.classification GENETICA es_ES
dc.title Location of chlorogenic acid biosynthesis pathway and poliphenol oxidase genes in a new interspecific anchored linkage map of eggplant es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1186/s12870-014-0350-z
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2012-34213/ES/NUEVAS ESTRATEGIAS PARA LA MEJORA DE LA CALIDAD NUTRACEUTICA DE LA BERENJENA/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Gramazio, P.; Prohens Tomás, J.; Plazas Ávila, MDLO.; Andújar Pérez, I.; Herraiz García, FJ.; Castillo Lorenzo, E.; Knapp, S.... (2014). Location of chlorogenic acid biosynthesis pathway and poliphenol oxidase genes in a new interspecific anchored linkage map of eggplant. BMC Plant Biology. 14(350):1-15. https://doi.org/10.1186/s12870-014-0350-z es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1186/s12870-014-0350-z es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 15 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 14 es_ES
dc.description.issue 350 es_ES
dc.relation.senia 283335 es_ES
dc.identifier.pmid 25491265 en_EN
dc.identifier.pmcid PMC4279458 en_EN
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Universitat Politècnica de València es_ES
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