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Detection and enumeration of viable Listeria monocytogenes cells from ready-to-eat and processed vegetable foods by culture and DVC-FISH

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Detection and enumeration of viable Listeria monocytogenes cells from ready-to-eat and processed vegetable foods by culture and DVC-FISH

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dc.contributor.author Moreno Trigos, Mª Yolanda es_ES
dc.contributor.author Sánchez Contreras, Javier es_ES
dc.contributor.author Montes Estellés, Rosa Mª es_ES
dc.contributor.author García Hernández, Jorge es_ES
dc.contributor.author Ballesteros, Lorena es_ES
dc.contributor.author Ferrús Pérez, Mª Antonia es_ES
dc.date.accessioned 2016-05-02T10:14:18Z
dc.date.available 2016-05-02T10:14:18Z
dc.date.issued 2012-10
dc.identifier.issn 0956-7135
dc.identifier.uri http://hdl.handle.net/10251/63321
dc.description.abstract Listeria monocytogenes is an important agent of foodborne disease, showing low prevalence but high mortality. There is evidence that vegetables are important vehicles of transmission, especially those minimally processed (fresh, fresh-cut vegetables under modified atmosphere packaging (MAP) or frozen) and directly exposed to consumers. The aim of this work was to detect and enumerate viable . L. . monocytogenes cells by culture and molecular methods from vegetable foods.A total of 191 vegetable samples (fresh, frozen and fresh-cut under modified atmosphere packaging) were studied. . L. monocytogenes was detected and identified by selective plating, PCR and DVC-FISH.An isolation rate of 4.19% was obtained by culture, with a higher incidence in frozen vegetables. Six isolates belonged to serotype 1/2a and 2 to 4b. Counts were below <100 cfu/g for the eight positive samples, according to the food safety criteria established for the RTE by Commission Regulation EC No 1441/2007.Multiplex PCR method yielded a greater number of positive samples (10.47%). DVC-FISH technique determined that viable cells of L. monocytogenes were present in 32.98% of the samples, containing up to 4.97 log 10 viable cells/g. © 2012 Elsevier Ltd. es_ES
dc.description.sponsorship This work was supported by the grant AGL2008-05275-C03-02 (national and FEDER funds) from Ministerio de Ciencia e Innovacion, Spain. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation Ministerio de Ciencia e Innovacion, Spain AGL2008-05275-C03-02 es_ES
dc.relation.ispartof Food Control es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Detection es_ES
dc.subject DVC-FISH es_ES
dc.subject Enumeration es_ES
dc.subject Listeria monocytogenes es_ES
dc.subject Vegetables es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.title Detection and enumeration of viable Listeria monocytogenes cells from ready-to-eat and processed vegetable foods by culture and DVC-FISH es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.foodcont.2012.04.017
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Ingeniería del Agua y del Medio Ambiente - Institut Universitari d'Enginyeria de l'Aigua i Medi Ambient es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Moreno Trigos, MY.; Sánchez Contreras, J.; Montes Estellés, RM.; García Hernández, J.; Ballesteros, L.; Ferrús Pérez, MA. (2012). Detection and enumeration of viable Listeria monocytogenes cells from ready-to-eat and processed vegetable foods by culture and DVC-FISH. Food Control. 27(2):374-379. doi:10.1016/j.foodcont.2012.04.017 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.foodcont.2012.04.017 es_ES
dc.description.upvformatpinicio 374 es_ES
dc.description.upvformatpfin 379 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 27 es_ES
dc.description.issue 2 es_ES
dc.relation.senia 231540 es_ES


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