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Persistence of Listeria monocytogenes strains in a frozen vegetables processing plant determined by serotyping and REP-PCR

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Persistence of Listeria monocytogenes strains in a frozen vegetables processing plant determined by serotyping and REP-PCR

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dc.contributor.author Ballesteros Calabuig, Lorena es_ES
dc.contributor.author Moreno Trigos, Mª Yolanda es_ES
dc.contributor.author Cuesta Amat, Gonzalo es_ES
dc.contributor.author Rodrigo, Alejandro es_ES
dc.contributor.author Tomás, David es_ES
dc.contributor.author Hernández Pérez, Manuel es_ES
dc.contributor.author Ferrús Pérez, Mª Antonia es_ES
dc.contributor.author García Hernández, Jorge es_ES
dc.date.accessioned 2016-05-02T10:53:14Z
dc.date.available 2016-05-02T10:53:14Z
dc.date.issued 2011-05
dc.identifier.issn 0950-5423
dc.identifier.uri http://hdl.handle.net/10251/63330
dc.description.abstract Seventy presumptive Listeria monocytogenes strains isolated from frozen vegetable products in a processing plant during a period of six months were characterized by serology, biochemical tests (API system), multiplex PCR, virulence characteristics and REP-PCR analysis. Most isolates belonged to 1/2a (62 strains) and 1/2b (3 strains) serotypes, the most common isolated from foods. Amplification of virulence gene lmo2821 was positive in 51 of the PCR-confirmed L. monocytogenes strains. Automated REP-PCR of L. monocytogenes isolates yielded 17 different patterns, formed by 70 to 80 different bands ranging from 150 to 7000 bp. A total of three genetic groups were defined at 82% homology degree. At this level, serovars 4b and 1/2b strains were discriminated from the rest of strains. Two REP-PCR patterns were frequently found for isolates sharing the same serotype and biochemical profile. These isolates were obtained from different vegetables during the sampling period, what clearly suggests the persistence of some L. monocytogenes strains in the processing plant. While the multiplex PCR assay applied in this work provided an accurate and rapid method for species identification, REP-PCR was confirmed as a rapid and reliable method for L. monocytogenes subtyping, providing useful information for epidemiological or risk assessment studies, as well as for tracking surveys in food processing plants. es_ES
dc.description.sponsorship This work was supported by grant AGL2008-05275-C03-02 from Ministerio de Ciencia e Innovacion, Spain. en_EN
dc.language Inglés es_ES
dc.publisher Wiley-Blackwell es_ES
dc.relation Ministerio de Ciencia e Innovacion, Spain AGL2008-05275-C03-02 es_ES
dc.relation.ispartof International Journal of Food Science and Technology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Listeria monocytogenes es_ES
dc.subject REP-PCR es_ES
dc.subject Multiplex PCR es_ES
dc.subject Virulence es_ES
dc.subject Serology es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.title Persistence of Listeria monocytogenes strains in a frozen vegetables processing plant determined by serotyping and REP-PCR es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/j.1365-2621.2011.02595.x
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Ingeniería del Agua y del Medio Ambiente - Institut Universitari d'Enginyeria de l'Aigua i Medi Ambient es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Ballesteros Calabuig, L.; Moreno Trigos, MY.; Cuesta Amat, G.; Rodrigo, A.; Tomás, D.; Hernández Pérez, M.; Ferrús Pérez, MA.... (2011). Persistence of Listeria monocytogenes strains in a frozen vegetables processing plant determined by serotyping and REP-PCR. International Journal of Food Science and Technology. 46(5):1109-1112. doi:10.1111/j.1365-2621.2011.02595.x es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1111/j.1365-2621.2011.02595.x es_ES
dc.description.upvformatpinicio 1109 es_ES
dc.description.upvformatpfin 1112 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 46 es_ES
dc.description.issue 5 es_ES
dc.relation.senia 210891 es_ES


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