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Improving the effectiveness of amiRNA-mediated resistance against Turnip mosaic virus by combining two amiRNAs or by targeting highly conserved viral genomic regions

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Improving the effectiveness of amiRNA-mediated resistance against Turnip mosaic virus by combining two amiRNAs or by targeting highly conserved viral genomic regions

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dc.contributor.author Lafforgue, Guillaume es_ES
dc.contributor.author Martínez, Fernando es_ES
dc.contributor.author Niu, Qi Wen es_ES
dc.contributor.author Chua, Nam Hai es_ES
dc.contributor.author Daros Arnau, Jose Antonio es_ES
dc.contributor.author Elena Fito, Santiago Fco es_ES
dc.date.accessioned 2016-05-04T07:06:34Z
dc.date.available 2016-05-04T07:06:34Z
dc.date.issued 2013-07
dc.identifier.issn 0022-538X
dc.identifier.uri http://hdl.handle.net/10251/63474
dc.description.abstract [EN] A drawback of recent antiviral therapies based on the transgenic expression of artificial microRNAs (amiRs) is the ease with which viruses generate escape mutations. Here, we show two alternative strategies for improving the effectiveness of resistance in plants. First, we expressed two amiRs complementary to independent targets in the viral genome, and second, we designed amiRs complementary to highly conserved RNA motifs in the viral genome. es_ES
dc.description.sponsorship This work was supported by grants RGP12/2008 from the Human Frontier Science Program Organization, PROMETEO2010/019 from the Generalitat Valenciana, and BFU2012-30805 from the Spanish Ministerio de Economia y Competitividad (MINECO) to S.F.E. and by grants 2010TW0015 from CSIC and BIO2011-26741 from MINECO to J.-A.D.F.M. was supported by a fellowship from the Universidad Politecnica de Valencia. en_EN
dc.language Inglés es_ES
dc.publisher American Society for Microbiology es_ES
dc.relation.ispartof Journal of Virology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject RNA INTERFERENCE es_ES
dc.subject ESCAPE es_ES
dc.subject REPLICATION es_ES
dc.subject INHIBITION es_ES
dc.subject EXPRESSION es_ES
dc.title Improving the effectiveness of amiRNA-mediated resistance against Turnip mosaic virus by combining two amiRNAs or by targeting highly conserved viral genomic regions es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1128/JVI.00914-13
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2010%2F019/ES/Implicaciones evolutivas de la supresión del silenciamiento del RNA por parte de proteína virales/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/HFSP//RGP0012%2F2008/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BFU2012-30805/ES/EVOLUTIONARY SYSTEMS VIROLOGY: EPISTASIS AND THE RUGGEDNESS OF ADAPTIVE LANDSCAPES, MUTATIONS IN REGULATORY SEQUENCES, AND THE HOST DETERMINANTS OF VIRAL FITNESS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/CSIC//2010TW0015/ES/Evaluation of the durability of artificial microRNA-mediated strategies for plant resistance to RNA viruses/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2011-26741/ES/PATOGENOS DE RNA DE PLANTAS: INTERACCION CON EL HUESPED Y DESARROLLO DE HERRAMIENTAS BIOTECNOLOGICAS/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Lafforgue, G.; Martínez, F.; Niu, QW.; Chua, NH.; Daros Arnau, JA.; Elena Fito, SF. (2013). Improving the effectiveness of amiRNA-mediated resistance against Turnip mosaic virus by combining two amiRNAs or by targeting highly conserved viral genomic regions. Journal of Virology. 87(14):8254-8256. https://doi.org/10.1128/JVI.00914-13 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1128/JVI.00914-13 es_ES
dc.description.upvformatpinicio 8254 es_ES
dc.description.upvformatpfin 8256 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 87 es_ES
dc.description.issue 14 es_ES
dc.relation.senia 258241 es_ES
dc.identifier.pmcid PMC3700214 en_EN
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Human Frontier Science Program Organization es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Consejo Superior de Investigaciones Científicas; National Science Council of Taiwan es_ES
dc.contributor.funder Universitat Politècnica de València es_ES
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