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Apical dominance in saffron and the involvement of the branching enzymes CCD7 and CCD8 in the control of bud sprouting

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Apical dominance in saffron and the involvement of the branching enzymes CCD7 and CCD8 in the control of bud sprouting

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dc.contributor.author Rubio Moraga, Angela es_ES
dc.contributor.author Ahrazem, Oussama es_ES
dc.contributor.author Pérez Clemente, Rosa María es_ES
dc.contributor.author Gomez Cadenas, Aurelio es_ES
dc.contributor.author Yoneyama, Koichi es_ES
dc.contributor.author López Ráez, Juan Antonio es_ES
dc.contributor.author Molina Romero, Rosa Victoria es_ES
dc.contributor.author Gómez Gómez, Lourdes es_ES
dc.date.accessioned 2016-05-10T10:27:28Z
dc.date.available 2016-05-10T10:27:28Z
dc.date.issued 2014-06-19
dc.identifier.issn 1471-2229
dc.identifier.uri http://hdl.handle.net/10251/63842
dc.description.abstract [EN] Background: In saffron (Crocus sativus), new corms develop at the base of every shoot developed from the maternal corm, a globular underground storage stem. Since the degree of bud sprouts influences the number and size of new corms, and strigolactones (SLs) suppress growth of pre-formed axillary bud, it was considered appropriate to investigate SL involvement in physiology and molecular biology in saffron. We focused on two of the genes within the SL pathway, CCD7 and CCD8, encoding carotenoid cleavage enzymes required for the production of SLs. Results: The CsCCD7 and CsCCD8 genes are the first ones isolated and characterized from a non-grass monocotyledonous plant. CsCCD7 and CsCCD8 expression showed some overlapping, although they were not identical. CsCCD8 was highly expressed in quiescent axillary buds and decapitation dramatically reduced its expression levels, suggesting its involvement in the suppression of axillary bud outgrowth. Furthermore, in vitro experiments showed also the involvement of auxin, cytokinin and jasmonic acid on the sprouting of axillary buds from corms in which the apical bud was removed. In addition, CsCCD8 expression, but not CsCCD7, was higher in the newly developed vascular tissue of axillary buds compared to the vascular tissue of the apical bud. Conclusions: We showed that production and transport of auxin in saffron corms could act synergistically with SLs to arrest the outgrowth of the axillary buds, similar to the control of above-ground shoot branching. In addition, jasmonic acid seems to play a prominent role in bud dormancy in saffron. While cytokinins from roots promote bud outgrowth. In addition the expression results of CsCCD8 suggest that SLs could positively regulate procambial activity and the development of new vascular tissues connecting leaves with the mother corm. es_ES
dc.description.sponsorship We thank J. Argandona (Instituto Botanico, Universidad de Castilla-La Mancha, Albacete, Spain) for excellent technical support, and K.A. Walsh for language revision. The laboratory is funded by the Spanish Ministerio de Ciencia e Innovacion (BIO2009-07803) and participates in the IBERCAROT network (112RT0445). Dr. Ahrazem was funded by FPCYTA through the INCRECYT Programme. en_EN
dc.language Inglés es_ES
dc.publisher BioMed Central es_ES
dc.relation.ispartof BMC Plant Biology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Auxin es_ES
dc.subject Buds es_ES
dc.subject Carotenoid cleavage oxygenases es_ES
dc.subject Corm es_ES
dc.subject Saffron es_ES
dc.subject Strigolactones es_ES
dc.subject.classification BIOLOGIA VEGETAL es_ES
dc.subject.classification FISIOLOGIA VEGETAL es_ES
dc.title Apical dominance in saffron and the involvement of the branching enzymes CCD7 and CCD8 in the control of bud sprouting es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1186/1471-2229-14-171
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2009-07803/ES/Glucosiltransferasas Y Glucosidasas (Cazy Enzymes) De Flavonoides Y Apocarotenoides De Crocus Sativus/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/CYTED//112RT0445/ES/RED IBEROAMERICANA PARA EL ESTUDIO DE NUEVOS CAROTENOIDES BIOACTIVOS COMO INGREDIENTES DE ALIMENTOS (IBERCAROT)/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Producción Vegetal - Departament de Producció Vegetal es_ES
dc.description.bibliographicCitation Rubio Moraga, A.; Ahrazem, O.; Pérez Clemente, RM.; Gomez Cadenas, A.; Yoneyama, K.; López Ráez, JA.; Molina Romero, RV.... (2014). Apical dominance in saffron and the involvement of the branching enzymes CCD7 and CCD8 in the control of bud sprouting. BMC Plant Biology. 14:171-185. https://doi.org/10.1186/1471-2229-14-171 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1186/1471-2229-14-171 es_ES
dc.description.upvformatpinicio 171 es_ES
dc.description.upvformatpfin 185 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 14 es_ES
dc.relation.senia 280208 es_ES
dc.identifier.pmid 24947472 en_EN
dc.identifier.pmcid PMC4077219 en_EN
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder CYTED Ciencia y Tecnología para el Desarrollo es_ES
dc.contributor.funder Fundación Parque Científico Tecnológico de Albacete es_ES


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