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Assessing the genetic variation of Ty-1 and Ty-3 alleles conferring resistance to Tomato Yellow Leaf Curl Virus in a broad tomato germplasm

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Assessing the genetic variation of Ty-1 and Ty-3 alleles conferring resistance to Tomato Yellow Leaf Curl Virus in a broad tomato germplasm

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Nombre: Caro et al. 2015_ ...
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dc.contributor.author Caro, Myluska es_ES
dc.contributor.author Verlaan, Maarten G. es_ES
dc.contributor.author Julián Rodríguez, Olga es_ES
dc.contributor.author Finkers, Richard es_ES
dc.contributor.author Wolters, Anne-Marie A. es_ES
dc.contributor.author Hutton, Samuel es_ES
dc.contributor.author Scott, John W. es_ES
dc.contributor.author Kormelink, Richard es_ES
dc.contributor.author Visser, Richard es_ES
dc.contributor.author Díez Niclós, Mª José Teresa de Jesús es_ES
dc.contributor.author Pérez de Castro, Ana María es_ES
dc.contributor.author Bai, Yuling
dc.date.accessioned 2016-05-11T13:06:59Z
dc.date.available 2016-05-11T13:06:59Z
dc.date.issued 2015
dc.identifier.issn 1380-3743
dc.identifier.uri http://hdl.handle.net/10251/63922
dc.description The online version of this article (doi:10.1007/s11032-015-0329-y) contains supplementary material, which is available to authorized users. es_ES
dc.description.abstract [EN] Tomato yellow leaf curl virus (TYLCV) hampers tomato production worldwide. Our previous studies have focussed on mapping and ultimately cloning of the TYLCV resistance genes Ty-1 and Ty-3. Both genes are derived from Solanum chilense and were shown to be allelic. They code for an RNA-dependent RNA polymerase (RDR) belonging to the RDR gamma type defined by a DFDGD catalytic domain. In this study, we first fine-mapped the TYLCV resistance in S. chilense LA1932, LA1960 and LA1971. Results showed that chromosomal intervals of the causal genes in these TYLCV-resistant accessions overlap and cover the region where Ty-1/Ty-3 is located. Further, virus-induced gene silencing was used to silence Ty-1/Ty-3 in tomato lines carrying TYLCV resistance introgressed from S. chilense LA1932, LA1938 and LA1971. Results showed that silencing Ty-1/Ty-3 compromised the resistance in lines derived from S. chilense LA1932 and LA1938. The LA1971-derived material remained resistant upon silencing Ty-1/Ty-3. Further, we studied the allelic variation of the Ty-1/Ty-3 gene by examining cDNA sequences from nine S. chilense-derived lines/accessions and more than 80 tomato cultivars, landraces and accessions of related wild species. The DFDGD catalytic domain of the Ty-1/Ty-3 gene is conserved among all tomato lines and species analysed. In addition, the 12 base pair insertion at the 5-prime part of the Ty-1/Ty-3 gene was found not to be specific for the TYLCV resistance allele. However, compared with the susceptible ty-1 allele, the Ty-1/Ty-3 allele is characterized by three specific amino acids shared by seven TYLCV-resistant S. chilense accessions or derived lines. Thus, Ty-1/Ty-3-specific markers can be developed based on these polymorphisms. Elevated transcript levels were observed for all tested S. chilense RDR alleles (both Ty-1 and ty-1 alleles), demonstrating that elevated expression level is not a good selection criterion for a functional Ty-1/Ty-3 allele. es_ES
dc.description.sponsorship The infectious TYLCV clone was kindly provided by Professor Eduardo Rodriguez Bejarano (Universidad de Malaga). We thank Dick Lohuis for his help with agro-infiltrations, Marc Hendriks and Marjon Arens for RNA isolation and sequencing. This project was financed by the Centre for BioSystems Genomics (CBSG), which is part of the Netherlands Genomics Initiative/Netherlands Organization for Scientific Research (http://www.cbsg.nl). Olga Julian was granted a scholarship by Generalitat Valenciana. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. en_EN
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation.ispartof Molecular Breeding es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Breeding es_ES
dc.subject Resistance es_ES
dc.subject RNA-dependent es_ES
dc.subject RNA polymerase (RDR) es_ES
dc.subject Tomato yellow leaf curl virus (TYLCV) es_ES
dc.subject Virus-induced gene silencing (VIGS) es_ES
dc.subject.classification GENETICA es_ES
dc.title Assessing the genetic variation of Ty-1 and Ty-3 alleles conferring resistance to Tomato Yellow Leaf Curl Virus in a broad tomato germplasm es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s11032-015-0329-y
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Caro, M.; Verlaan, MG.; Julián Rodríguez, O.; Finkers, R.; Wolters, AA.; Hutton, S.; Scott, JW.... (2015). Assessing the genetic variation of Ty-1 and Ty-3 alleles conferring resistance to Tomato Yellow Leaf Curl Virus in a broad tomato germplasm. Molecular Breeding. 35. doi:10.1007/s11032-015-0329-y es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1007/s11032-015-0329-y es_ES
dc.description.upvformatpinicio 132 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 35 es_ES
dc.relation.senia 292352 es_ES
dc.identifier.eissn 1572-9788
dc.identifier.pmid 26028987 en_EN
dc.identifier.pmcid PMC4442973 en_EN
dc.contributor.funder Netherlands Organization for Scientific Research es_ES
dc.contributor.funder Generalitat Valenciana es_ES
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