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Role of atrial tissue remodeling on rotor dynamics an in vitro study

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Role of atrial tissue remodeling on rotor dynamics an in vitro study

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dc.contributor.author Climent, A.M. es_ES
dc.contributor.author Guillem Sánchez, María Salud es_ES
dc.contributor.author Fuentes, L. es_ES
dc.contributor.author Lee, P. es_ES
dc.contributor.author Bollensdorff, C. es_ES
dc.contributor.author Fernandez-Santos, M.E. es_ES
dc.contributor.author Suarez-Sancho, S. es_ES
dc.contributor.author Sanz-Ruiz, R. es_ES
dc.contributor.author Sanchez, P.L. es_ES
dc.contributor.author Atienza, F. es_ES
dc.contributor.author Fernandez-Aviles, F. es_ES
dc.date.accessioned 2016-05-17T11:42:23Z
dc.date.available 2016-05-17T11:42:23Z
dc.date.issued 2015-12-01
dc.identifier.issn 0363-6135
dc.identifier.uri http://hdl.handle.net/10251/64249
dc.description.abstract The objective of this article is to present an in vitro model of atrial cardiac tissue that could serve to study the mechanisms of remodeling related to atrial fibrillation (AF). We analyze the modification on gene expression and modifications on rotor dynamics following tissue remodeling. Atrial murine cells (HL-1 myocytes) were maintained in culture after the spontaneous initiation of AF and analyzed at two time points: 3.1 +/- 1.3 and 9.7 +/- 0.5 days after AF initiation. The degree of electrophysiological remodeling (i.e., relative gene expression of key ion channels) and structural inhomogeneity was compared between early and late cell culture times both in nonfibrillating and fibrillating cell cultures. In addition, the electrophysiological characteristics of in vitro fibrillation [e.g., density of phase singularities (PS/cm2), dominant frequency, and rotor meandering] analyzed by means of optical mapping were compared with the degree of electrophysiological remodeling. Fibrillating cell cultures showed a differential ion channel gene expression associated with atrial tissue remodeling (i.e., decreased SCN5A, CACN1C, KCND3, and GJA1 and increased KCNJ2) not present in nonfibrillating cell cultures. Also, fibrillatory complexity was increased in late- vs. early stage cultures (1.12 +/- 0.14 vs. 0.43 +/- 0.19 PS/cm(2), P < 0.01), which was associated with changes in the electrical reentrant patterns (i.e., decrease in rotor tip meandering and increase in wavefront curvature). HL-1 cells can reproduce AF features such as electrophysiological remodeling and an increased complexity of the electrophysiological behavior associated with the fibrillation time that resembles those occurring in patients with chronic AF. es_ES
dc.description.sponsorship This work was supported in part by grants from the Spanish Ministry of Science and Innovation (PLE2009-0152), the Instituto de Salud Carlos III (Ministry of Economy and Competitiveness, Spain: PI13-01882, PI13-00903, and TEC2013-50391-EXP), and the Red de Investigacion Cardiovacular (RIC) from Instituto de Salud Carlos III (Ministry of Economy and Competitiveness, Spain). en_EN
dc.language Inglés es_ES
dc.publisher American Physiological Society es_ES
dc.relation This work was supported in part by grants from the Spanish Ministry of Science and Innovation (PLE2009-0152), the Instituto de Salud Carlos III (Ministry of Economy and Competitiveness, Spain: PI13-01882, PI13-00903, and TEC2013-50391-EXP), and the Red de Investigacion Cardiovacular (RIC) from Instituto de Salud Carlos III (Ministry of Economy and Competitiveness, Spain). es_ES
dc.relation.ispartof AJP - Heart and Circulatory Physiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Atrial Fibrillation es_ES
dc.subject Optical mapping es_ES
dc.subject.classification TECNOLOGIA ELECTRONICA es_ES
dc.title Role of atrial tissue remodeling on rotor dynamics an in vitro study es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1152/ajpheart.00055.2015
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Electrónica - Departament d'Enginyeria Electrònica es_ES
dc.description.bibliographicCitation Climent, A.; Guillem Sánchez, MS.; Fuentes, L.; Lee, P.; Bollensdorff, C.; Fernandez-Santos, M.; Suarez-Sancho, S.... (2015). Role of atrial tissue remodeling on rotor dynamics an in vitro study. AJP - Heart and Circulatory Physiology. 309(11):H1964-H1973. doi:10.1152/ajpheart.00055.2015 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1152/ajpheart.00055.2015 es_ES
dc.description.upvformatpinicio H1964 es_ES
dc.description.upvformatpfin H1973 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 309 es_ES
dc.description.issue 11 es_ES
dc.relation.senia 305193 es_ES


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