- -

Direct hapten-linked multiplexed immunoassays on polycarbonate surface

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

Direct hapten-linked multiplexed immunoassays on polycarbonate surface

Show simple item record

Files in this item

dc.contributor.author Tamarit López, Jesús es_ES
dc.contributor.author Morais Ezquerro, Sergi Beñat es_ES
dc.contributor.author Puchades Pla, Rosa es_ES
dc.contributor.author Maquieira Catala, Ángel es_ES
dc.date.accessioned 2016-05-23T12:06:36Z
dc.date.available 2016-05-23T12:06:36Z
dc.date.issued 2011-01-15
dc.identifier.issn 0956-5663
dc.identifier.uri http://hdl.handle.net/10251/64608
dc.description.abstract Direct hapten-linked multiplexed immunoassay is developed on the polycarbonate surface of standard Digital Versatile Discs (DVDs) for six compounds of environmental concern, as proof of concept. Carboxylated haptens are directly linked to the aminated polycarbonate surface through carbodiimide/succinimide coupling. The modified DVD surface maintained its physical and optical properties. Multiplexed assay reached detection limits down to 0.1¿g/L for chlorpyrifos, 2,4,5-trichlorophenoxypropionic acid, sulfathiazole and sulfasalazine and down to 1.0¿g/L for fenthion and malathion. This approach presents advantages such as the improvement in sensitivity in comparison to protein-hapten conjugate format for all the studied analytes and the absence of cross-interference effects, allowing high throughput multianalysis on the same surface. Also, a comparison of the performance of two sensing strategies indicated that DVD disc and drive approach turned out in a simpler mode, the assays being more reproducible and with higher signal to noise ratios. © 2010 Elsevier B.V. es_ES
dc.description.sponsorship This research was funded through FEDER projects CTQ2007-64735/BQU (CICYT, Spain) and ACOMPGVA-2009/650 (GVA, Spain). The Spanish MEC provided J.T.-L. with a grant for his PhD studies. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation FEDER (CICYT, Spain)/CTQ2007-64735/BQU es_ES
dc.relation FEDER (GVA, Spain)/ACOMPGVA-2009/650 es_ES
dc.relation Spanish MEC es_ES
dc.relation.ispartof Biosensors and Bioelectronics es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Digital versatile disc es_ES
dc.subject Direct hapten-linked immunoassay es_ES
dc.subject Microarray es_ES
dc.subject Multiplexed analysis es_ES
dc.subject Polycarbonate es_ES
dc.subject Analytes es_ES
dc.subject Chlorpyrifos es_ES
dc.subject Detection limits es_ES
dc.subject Environmental concerns es_ES
dc.subject Fenthion es_ES
dc.subject High throughput es_ES
dc.subject Interference effects es_ES
dc.subject Multianalysis es_ES
dc.subject Proof of concept es_ES
dc.subject Sulfathiazole es_ES
dc.subject Assays es_ES
dc.subject Data storage equipment es_ES
dc.subject Immunology es_ES
dc.subject Optical properties es_ES
dc.subject Plastic products es_ES
dc.subject Signal to noise ratio es_ES
dc.subject Videodisks es_ES
dc.subject Multiplexing es_ES
dc.subject Cyanamide es_ES
dc.subject Hapten es_ES
dc.subject Malathion es_ES
dc.subject Salazosulfapyridine es_ES
dc.subject Succinimide es_ES
dc.subject Water es_ES
dc.subject Article es_ES
dc.subject Chemical modification es_ES
dc.subject Compact disk es_ES
dc.subject Controlled study es_ES
dc.subject Digital versatile device es_ES
dc.subject Direct hapten linked multiplexed immunoassay es_ES
dc.subject Hapten carrier complex es_ES
dc.subject High throughput screening es_ES
dc.subject Immunoassay es_ES
dc.subject Immunocytochemistry es_ES
dc.subject Physical chemistry es_ES
dc.subject Process development es_ES
dc.subject Quantitative analysis es_ES
dc.subject Sensitivity analysis es_ES
dc.subject Signal noise ratio es_ES
dc.subject Surface property es_ES
dc.subject Compact Disks es_ES
dc.subject Environmental Monitoring es_ES
dc.subject Environmental Pollutants es_ES
dc.subject Equipment Design es_ES
dc.subject Equipment Failure Analysis es_ES
dc.subject Haptens es_ES
dc.subject Polycarboxylate Cement es_ES
dc.subject Surface Properties es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Direct hapten-linked multiplexed immunoassays on polycarbonate surface es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.bios.2010.08.066
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation Tamarit López, J.; Morais Ezquerro, SB.; Puchades Pla, R.; Maquieira Catala, Á. (2011). Direct hapten-linked multiplexed immunoassays on polycarbonate surface. Biosensors and Bioelectronics. 26(5):2694-2698. doi:10.1016/j.bios.2010.08.066 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.bios.2010.08.066 es_ES
dc.description.upvformatpinicio 2694 es_ES
dc.description.upvformatpfin 2698 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 26 es_ES
dc.description.issue 5 es_ES
dc.relation.senia 212768


This item appears in the following Collection(s)

Show simple item record