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Toxicity Mechanisms of the Food Contaminant Citrinin: Application of a Quantitative Yeast Model

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Toxicity Mechanisms of the Food Contaminant Citrinin: Application of a Quantitative Yeast Model

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dc.contributor.author Pascual-Ahuir Giner, María Desamparados es_ES
dc.contributor.author Vanacloig Pedrós, María Elena es_ES
dc.contributor.author Proft, Markus Hans es_ES
dc.date.accessioned 2016-05-23T14:58:33Z
dc.date.available 2016-05-23T14:58:33Z
dc.date.issued 2014-05
dc.identifier.issn 2072-6643
dc.identifier.uri http://hdl.handle.net/10251/64625
dc.description.abstract Mycotoxins are important food contaminants and a serious threat for human nutrition. However, in many cases the mechanisms of toxicity for this diverse group of metabolites are poorly understood. Here we apply live cell gene expression reporters in yeast as a quantitative model to unravel the cellular defense mechanisms in response to the mycotoxin citrinin. We find that citrinin triggers a fast and dose dependent activation of stress responsive promoters such as GRE2 or SOD2. More specifically, oxidative stress responsive pathways via the transcription factors Yap1 and Skn7 are critically implied in the response to citrinin. Additionally, genes in various multidrug resistance transport systems are functionally involved in the resistance to citrinin. Our study identifies the antioxidant defense as a major physiological response in the case of citrinin. In general, our results show that the use of live cell gene expression reporters in yeast are a powerful tool to identify toxicity targets and detoxification mechanisms of a broad range of food contaminants relevant for human nutrition. es_ES
dc.description.sponsorship This work was supported by Ministerio de Economia y Competitividad grant BFU2011-23326. We thank the Fond for Open Access Publication from Consejo Superior de Investigaciones Cientificas for supporting publication costs of this article. en_EN
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof Nutrients es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Citrinin es_ES
dc.subject Oxidative stress es_ES
dc.subject Yeast es_ES
dc.subject Mycotoxins es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Toxicity Mechanisms of the Food Contaminant Citrinin: Application of a Quantitative Yeast Model es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/nu6052077
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BFU2011-23326/ES/REGULACION DE LA CROMATINA Y DE LA ESTRUCTURA MITOCONDRIAL EN RESPUESTA A ESTRES OSMOTICO/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Pascual-Ahuir Giner, MD.; Vanacloig Pedrós, ME.; Proft, MH. (2014). Toxicity Mechanisms of the Food Contaminant Citrinin: Application of a Quantitative Yeast Model. Nutrients. 6(5):2077-2087. https://doi.org/10.3390/nu6052077 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.3390/nu6052077 es_ES
dc.description.upvformatpinicio 2077 es_ES
dc.description.upvformatpfin 2087 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 6 es_ES
dc.description.issue 5 es_ES
dc.relation.senia 280731 es_ES
dc.identifier.pmid 24858409 en_EN
dc.identifier.pmcid PMC4042565 en_EN
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Consejo Superior de Investigaciones Científicas es_ES
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