Alió Del Barrio, JL.; Chiesa, M.; Gallego Ferrer, G.; Garagorri, N.; Briz, N.; Fernández Delgado, J.; Sancho-Tello Valls, M.... (2015). Biointegration of corneal macroporous membranes based on poly(ethyl acrylate) copolymers in an experimental animal model. Journal of Biomedical Materials Research Part A. 103A(3):1106-1118. doi:10.1002/jbm.a.35249
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/64706
Title:
|
Biointegration of corneal macroporous membranes based on poly(ethyl acrylate) copolymers in an experimental animal model
|
Author:
|
Alió del Barrio, Jorge L.
Chiesa, Massimo
Gallego Ferrer, Gloria
Garagorri, Nerea
Briz, Nerea
Fernández Delgado, Jorge
Sancho-Tello Valls, María
Carda Botella, Carmen
García-Tuñón, Ignacio
Bataille, Laurent
Rodríguez, Alejandra
Arnalich Montiel, Francisco
Gómez Ribelles, José Luís
Antolinos Turpín, Carmen María
Gómez Tejedor, José Antonio
|
UPV Unit:
|
Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular
Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada
Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada
|
Issued date:
|
|
Abstract:
|
Currently available keratoprosthesis models (nonbiological corneal substitutes) have a less than 75% graft survival rate at 2 years. We aimed at developing a model for keratoprosthesis based on the use of poly(ethyl acrylate) ...[+]
Currently available keratoprosthesis models (nonbiological corneal substitutes) have a less than 75% graft survival rate at 2 years. We aimed at developing a model for keratoprosthesis based on the use of poly(ethyl acrylate) (PEA)-based copolymers, extracellular matrix-protein coating and colonization with adipose-derived mesenchymal stem cells. Human adipose tissue derived mesenchymal stem cells
(h-ADASC) colonization efficiency of seven PEA-based copolymers in combination with four extracellular matrix coatings were evaluated in vitro. Then, macroporous membranes composed of the optimal PEA subtypes and coating
proteins were implanted inside rabbit cornea. After a 3-month follow-up, the animals were euthanized, and the clinical and histological biointegration of the implanted material were assessed. h-ADASC adhered and survived when cultured
in all PEA-based macroporous membranes. The addition of high hydrophilicity to PEA membranes decreased h-ADASC colonization in vitro. PEA-based copolymer containing 10% hydroxyethyl acrylate (PEA-HEA10) or 10% acrylic
acid (PEA-AAc10) monomeric units showed the best cellular colonization rates. Collagen plus keratan sulfate-coated polymers demonstrated enhanced cellular colonization respect to fibronectin, collagen, or uncoated PEAs. In vivo implantation
of membranes resulted in an extrusion rate of 72% for PEA, 50% for PEA-AAc10, but remarkably of 0% for PEA-HEA10. h-ADASC survival was demonstrated in all the membranes after 3 months follow-up. A slight reduction in the extrusion
rate of h-ADASC colonized materials was observed. No significant differences between the groups with and without h-ADASC were detected respect to transparency or neovascularization. We propose PEA with low hydroxylation as a scaffold for the anchoring ring of future keratoprosthesis.
[-]
|
Subjects:
|
Poly(ethyl acrylate)
,
Adipose derived stem cells
,
Cornea
,
Macroporous membranes
,
Corneal scaffold
|
Copyrigths:
|
Reserva de todos los derechos
|
Source:
|
Journal of Biomedical Materials Research Part A. (issn:
1549-3296
) (eissn:
1552-4965
)
|
DOI:
|
10.1002/jbm.a.35249
|
Publisher:
|
Wiley: 12 months
|
Publisher version:
|
http://dx.doi.org/10.1002/jbm.a.35249
|
Thanks:
|
Contract grant sponsor: Fundacio Marato de TV3, Spain
|
Type:
|
Artículo
|