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Biointegration of corneal macroporous membranes based on poly(ethyl acrylate) copolymers in an experimental animal model

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Biointegration of corneal macroporous membranes based on poly(ethyl acrylate) copolymers in an experimental animal model

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dc.contributor.author Alió del Barrio, Jorge L. es_ES
dc.contributor.author Chiesa, Massimo es_ES
dc.contributor.author Gallego Ferrer, Gloria es_ES
dc.contributor.author Garagorri, Nerea es_ES
dc.contributor.author Briz, Nerea es_ES
dc.contributor.author Fernández Delgado, Jorge es_ES
dc.contributor.author Sancho-Tello Valls, María es_ES
dc.contributor.author Carda Botella, Carmen es_ES
dc.contributor.author García-Tuñón, Ignacio es_ES
dc.contributor.author Bataille, Laurent es_ES
dc.contributor.author Rodríguez, Alejandra es_ES
dc.contributor.author Arnalich Montiel, Francisco es_ES
dc.contributor.author Gómez Ribelles, José Luís es_ES
dc.contributor.author Antolinos Turpín, Carmen María es_ES
dc.contributor.author Gómez Tejedor, José Antonio es_ES
dc.date.accessioned 2016-05-25T13:43:01Z
dc.date.available 2016-05-25T13:43:01Z
dc.date.issued 2015-03
dc.identifier.issn 1549-3296
dc.identifier.uri http://hdl.handle.net/10251/64706
dc.description.abstract Currently available keratoprosthesis models (nonbiological corneal substitutes) have a less than 75% graft survival rate at 2 years. We aimed at developing a model for keratoprosthesis based on the use of poly(ethyl acrylate) (PEA)-based copolymers, extracellular matrix-protein coating and colonization with adipose-derived mesenchymal stem cells. Human adipose tissue derived mesenchymal stem cells (h-ADASC) colonization efficiency of seven PEA-based copolymers in combination with four extracellular matrix coatings were evaluated in vitro. Then, macroporous membranes composed of the optimal PEA subtypes and coating proteins were implanted inside rabbit cornea. After a 3-month follow-up, the animals were euthanized, and the clinical and histological biointegration of the implanted material were assessed. h-ADASC adhered and survived when cultured in all PEA-based macroporous membranes. The addition of high hydrophilicity to PEA membranes decreased h-ADASC colonization in vitro. PEA-based copolymer containing 10% hydroxyethyl acrylate (PEA-HEA10) or 10% acrylic acid (PEA-AAc10) monomeric units showed the best cellular colonization rates. Collagen plus keratan sulfate-coated polymers demonstrated enhanced cellular colonization respect to fibronectin, collagen, or uncoated PEAs. In vivo implantation of membranes resulted in an extrusion rate of 72% for PEA, 50% for PEA-AAc10, but remarkably of 0% for PEA-HEA10. h-ADASC survival was demonstrated in all the membranes after 3 months follow-up. A slight reduction in the extrusion rate of h-ADASC colonized materials was observed. No significant differences between the groups with and without h-ADASC were detected respect to transparency or neovascularization. We propose PEA with low hydroxylation as a scaffold for the anchoring ring of future keratoprosthesis. es_ES
dc.description.sponsorship Contract grant sponsor: Fundacio Marato de TV3, Spain en_EN
dc.language Inglés es_ES
dc.publisher Wiley: 12 months es_ES
dc.relation.ispartof Journal of Biomedical Materials Research Part A es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Poly(ethyl acrylate) es_ES
dc.subject Adipose derived stem cells es_ES
dc.subject Cornea es_ES
dc.subject Macroporous membranes es_ES
dc.subject Corneal scaffold es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title Biointegration of corneal macroporous membranes based on poly(ethyl acrylate) copolymers in an experimental animal model es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1002/jbm.a.35249
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//CEN-20091021/ES/CUSTOMATIZED EYE CARE. OFTALMOLOGÍA PERSONALIZADA Y MÍNIMAMENTE INVASIVA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MITURCO//IAP-560610-2008-0044/ES/ELABORACIÓN DE MEMBRANAS AUTÓLOGAS PARA EL TRATAMIENTO DE PATOLOGÍAS CORNEALES/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//SAF2010-19230/ES/MECANISMOS MOLECULARES INVOLUCRADOS EN LA REPROGRAMACON CELULAR DE LAS CELULAS GERMINALES PRIMORDIALES/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.description.bibliographicCitation Alió Del Barrio, JL.; Chiesa, M.; Gallego Ferrer, G.; Garagorri, N.; Briz, N.; Fernández Delgado, J.; Sancho-Tello Valls, M.... (2015). Biointegration of corneal macroporous membranes based on poly(ethyl acrylate) copolymers in an experimental animal model. Journal of Biomedical Materials Research Part A. 103A(3):1106-1118. https://doi.org/10.1002/jbm.a.35249 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1002/jbm.a.35249 es_ES
dc.description.upvformatpinicio 1106 es_ES
dc.description.upvformatpfin 1118 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 103A es_ES
dc.description.issue 3 es_ES
dc.relation.senia 267327 es_ES
dc.identifier.eissn 1552-4965
dc.contributor.funder Ministerio de Industria, Turismo y Comercio es_ES
dc.contributor.funder Fundació La Marató de TV3 es_ES


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