Borrell, YJ.; Gallego Albiach, V.; García Fernández, C.; Mazzeo ., I.; Pérez Igualada, LM.; Asturiano Nemesio, JF.; Carleos, CE.... (2011). Assessment of parental contributions to fast- and slow-growing progenies in the sea bream Sparus aurata L. using a new multiplex PCR. Aquaculture. 314(1-4):58-65. https://doi.org/10.1016/j.aquaculture.2011.01.028
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/64754
Title:
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Assessment of parental contributions to fast- and slow-growing progenies in the sea bream Sparus aurata L. using a new multiplex PCR
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Author:
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Borrell, Yaisel J.
Gallego Albiach, Victor
García Fernández, Carmen
Mazzeo ., Ilaria
Pérez Igualada, Luz María
Asturiano Nemesio, Juan Francisco
Carleos, Carlos E.
Vázquez, Emilia
Sánchez, José A.
Blanco, Gloria
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UPV Unit:
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Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal
Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal
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Issued date:
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Abstract:
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Molecular tools to assist breeding programs in the gilthead sea bream (Sparus aurata L.) are scarce. A new multiplex PCR technique (OVIDORPLEX), which amplifies nine known microsatellite markers, was developed in this work. ...[+]
Molecular tools to assist breeding programs in the gilthead sea bream (Sparus aurata L.) are scarce. A new multiplex PCR technique (OVIDORPLEX), which amplifies nine known microsatellite markers, was developed in this work. This multiplex system showed a high mean heterozygosity (>0.800) and a high mean number of alleles per marker (>14) when tested in two sea bream broodstocks (A: 40 breeders and B: 38 breeders). We tested this multiplex PCR for inferring parentage in a Spanish hatchery that graded the animals by size as part of their management procedure. The progeny of the broodstock were divided into fast- and slow-growth groups. Parentage studies revealed that this management procedure entailed a global reduction of the breeders' representation in progeny and that breeders' contributions were significantly unequal. Due to this, effective sample sizes fell to N ê¿13-14 for fast- and N ê¿18-24 for slow-growth progeny groups. These results imply a 3 to 4% rate of inbreeding per generation in the fast-growth group, which is more important to hatchery managers than the slow group. Not all the progeny were evaluated in this experiment (due to the discarding steps), and thus it is difficult to know if the phenotypic performance showed by the fast-growing progeny will be heritable. However, there were genetic differences between the differentiated growth progeny groups (fast vs. slow, F ST values=0.016 to 0.023; P<0.01). We also identified breeders with significantly different contributions to the fast- (10 breeders) or to the slow- (15 breeders) growth progeny groups. Our results demonstrated that this new multiplex PCR could be useful for quantitative programs (breeding programs, detection of QTL, inbreeding control or reconstruction of fish genealogies) to improve the aquaculture of the gilthead sea bream (S. aurata). © 2011 Elsevier B.V.
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Subjects:
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Inbreeding
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Microsatellite markers
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Multiplex PCR
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OVIDORPLEX
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Parentage
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Sparus aurata
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Allele
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Body size
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Breeding
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Brood stock
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Fish culture
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Fishery management
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Genealogy
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Genetic marker
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Genetic variation
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Hatchery
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Heterozygosity
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Molecular analysis
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Perciform
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Polymerase chain reaction
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Animalia
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Archosargus rhomboidalis
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Copyrigths:
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Reserva de todos los derechos
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Source:
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Aquaculture. (issn:
0044-8486
)
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DOI:
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10.1016/j.aquaculture.2011.01.028
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Publisher:
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Elsevier
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Publisher version:
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http://dx.doi.org/10.1016/j.aquaculture.2011.01.028
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Project ID:
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info:eu-repo/grantAgreement/MEC//AGL2006-13411-C03-03/ES/MEJORA GENETICA EN DORADA: APLICACION DE GENOMICA Y MARCADORES GENETICOS EN LA SELECCION DE REPRODUCTORES/
info:eu-repo/grantAgreement/MEC//AGL2006-13411-C03-01/ES/EVALUACION DE LOTES DE REPRODUCTORES DE DORADA EN BASE A SU RESISTENCIA AL ESTRES, SU TASA DE CRECIMIENTO Y SU CALIDAD DE CARNE./
info:eu-repo/grantAgreement/MEC//AGL2007-64040-C03-00/
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Thanks:
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This work was carried out in collaboration with the fish farm Granja Marina Safor, S.L. (Gandia, Valencia, Spain) and the hatchery Piscicultura Marina Mediterranea, S.L. (Burriana, Castellon, Spain). It was financed by ...[+]
This work was carried out in collaboration with the fish farm Granja Marina Safor, S.L. (Gandia, Valencia, Spain) and the hatchery Piscicultura Marina Mediterranea, S.L. (Burriana, Castellon, Spain). It was financed by JACUMAR (the PROGENSA project) and the Spanish Ministry of Science and Innovation (MICINN; National Program of Resources and Food and Agriculture Technologies, AGL2006-13411-C03-00, SELECTSPARUS, and AGL2007-64040-C03-00, SELECTBREAM, including European Regional Development Funds). V. Gallego was supported by a FPI scholarship financed by MICINN; C. Garcia-Fernandez was supported by a FPU scholarship financed by MICINN; and I. Mazzeo was supported by a FPI scholarship financed by Generalitat Valenciana. We are indebted to three anonymous referees and the journal editor for valuable comments.
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Type:
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Artículo
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