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Assessment of parental contributions to fast- and slow-growing progenies in the sea bream Sparus aurata L. using a new multiplex PCR

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Assessment of parental contributions to fast- and slow-growing progenies in the sea bream Sparus aurata L. using a new multiplex PCR

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dc.contributor.author Borrell, Yaisel J. es_ES
dc.contributor.author Gallego Albiach, Victor es_ES
dc.contributor.author García Fernández, Carmen es_ES
dc.contributor.author Mazzeo ., Ilaria es_ES
dc.contributor.author Pérez Igualada, Luz María es_ES
dc.contributor.author Asturiano Nemesio, Juan Francisco es_ES
dc.contributor.author Carleos, Carlos E. es_ES
dc.contributor.author Vázquez, Emilia es_ES
dc.contributor.author Sánchez, José A. es_ES
dc.contributor.author Blanco, Gloria es_ES
dc.date.accessioned 2016-05-26T08:54:31Z
dc.date.available 2016-05-26T08:54:31Z
dc.date.issued 2011-04-04
dc.identifier.issn 0044-8486
dc.identifier.uri http://hdl.handle.net/10251/64754
dc.description.abstract Molecular tools to assist breeding programs in the gilthead sea bream (Sparus aurata L.) are scarce. A new multiplex PCR technique (OVIDORPLEX), which amplifies nine known microsatellite markers, was developed in this work. This multiplex system showed a high mean heterozygosity (>0.800) and a high mean number of alleles per marker (>14) when tested in two sea bream broodstocks (A: 40 breeders and B: 38 breeders). We tested this multiplex PCR for inferring parentage in a Spanish hatchery that graded the animals by size as part of their management procedure. The progeny of the broodstock were divided into fast- and slow-growth groups. Parentage studies revealed that this management procedure entailed a global reduction of the breeders' representation in progeny and that breeders' contributions were significantly unequal. Due to this, effective sample sizes fell to N ê¿13-14 for fast- and N ê¿18-24 for slow-growth progeny groups. These results imply a 3 to 4% rate of inbreeding per generation in the fast-growth group, which is more important to hatchery managers than the slow group. Not all the progeny were evaluated in this experiment (due to the discarding steps), and thus it is difficult to know if the phenotypic performance showed by the fast-growing progeny will be heritable. However, there were genetic differences between the differentiated growth progeny groups (fast vs. slow, F ST values=0.016 to 0.023; P<0.01). We also identified breeders with significantly different contributions to the fast- (10 breeders) or to the slow- (15 breeders) growth progeny groups. Our results demonstrated that this new multiplex PCR could be useful for quantitative programs (breeding programs, detection of QTL, inbreeding control or reconstruction of fish genealogies) to improve the aquaculture of the gilthead sea bream (S. aurata). © 2011 Elsevier B.V. es_ES
dc.description.sponsorship This work was carried out in collaboration with the fish farm Granja Marina Safor, S.L. (Gandia, Valencia, Spain) and the hatchery Piscicultura Marina Mediterranea, S.L. (Burriana, Castellon, Spain). It was financed by JACUMAR (the PROGENSA project) and the Spanish Ministry of Science and Innovation (MICINN; National Program of Resources and Food and Agriculture Technologies, AGL2006-13411-C03-00, SELECTSPARUS, and AGL2007-64040-C03-00, SELECTBREAM, including European Regional Development Funds). V. Gallego was supported by a FPI scholarship financed by MICINN; C. Garcia-Fernandez was supported by a FPU scholarship financed by MICINN; and I. Mazzeo was supported by a FPI scholarship financed by Generalitat Valenciana. We are indebted to three anonymous referees and the journal editor for valuable comments. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation JACUMAR es_ES
dc.relation Spanish Ministry of Science and Innovation MICINN/AGL2006-13411-C03-00 es_ES
dc.relation AGL2007-64040-C03-00]; MICINN; Generalitat Valenciana es_ES
dc.relation Spanish Ministry of Science and Innovation MICINN/AGL2007-64040-C03-00 es_ES
dc.relation ERDF es_ES
dc.relation.ispartof Aquaculture es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Inbreeding es_ES
dc.subject Microsatellite markers es_ES
dc.subject Multiplex PCR es_ES
dc.subject OVIDORPLEX es_ES
dc.subject Parentage es_ES
dc.subject Sparus aurata es_ES
dc.subject Allele es_ES
dc.subject Body size es_ES
dc.subject Breeding es_ES
dc.subject Brood stock es_ES
dc.subject Fish culture es_ES
dc.subject Fishery management es_ES
dc.subject Genealogy es_ES
dc.subject Genetic marker es_ES
dc.subject Genetic variation es_ES
dc.subject Hatchery es_ES
dc.subject Heterozygosity es_ES
dc.subject Molecular analysis es_ES
dc.subject Perciform es_ES
dc.subject Polymerase chain reaction es_ES
dc.subject Animalia es_ES
dc.subject Archosargus rhomboidalis es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Assessment of parental contributions to fast- and slow-growing progenies in the sea bream Sparus aurata L. using a new multiplex PCR es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.aquaculture.2011.01.028
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Borrell, YJ.; Gallego Albiach, V.; García Fernández, C.; Mazzeo ., I.; Pérez Igualada, LM.; Asturiano Nemesio, JF.; Carleos, CE.... (2011). Assessment of parental contributions to fast- and slow-growing progenies in the sea bream Sparus aurata L. using a new multiplex PCR. Aquaculture. 314(1-4):58-65. doi:10.1016/j.aquaculture.2011.01.028 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1016/j.aquaculture.2011.01.028 es_ES
dc.description.upvformatpinicio 58 es_ES
dc.description.upvformatpfin 65 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 314 es_ES
dc.description.issue 1-4 es_ES
dc.relation.senia 193403 es_ES


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