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dc.contributor.author | Rosello Lleti, Esther | es_ES |
dc.contributor.author | Rivera, Miguel | es_ES |
dc.contributor.author | Cortés, Raquel | es_ES |
dc.contributor.author | Azorin, Inmaculada | es_ES |
dc.contributor.author | Sirera Pérez, Rafael | es_ES |
dc.contributor.author | Martínez Dolz, Luis | es_ES |
dc.contributor.author | Hove, Leif | es_ES |
dc.contributor.author | Cinca, Juan | es_ES |
dc.contributor.author | Lago, Francisca | es_ES |
dc.contributor.author | González Juanatey, José R. | es_ES |
dc.contributor.author | Salvador, Antonio | es_ES |
dc.contributor.author | Portolés, Manuel | es_ES |
dc.date.accessioned | 2016-06-01T10:00:21Z | |
dc.date.available | 2016-06-01T10:00:21Z | |
dc.date.issued | 2012-02-10 | |
dc.identifier.issn | 0006-291X | |
dc.identifier.uri | http://hdl.handle.net/10251/65039 | |
dc.description.abstract | [EN] We investigate for the first time the influence of heart failure (HF) on nucleolar organization and proteins in patients with ischemic (ICM) or dilated cardiomyopathy (DCM). A total of 71 human hearts from ICM (n= 38) and DCM (n= 27) patients, undergoing heart transplantation and control donors (n= 6), were analysed by western-blotting, RT-PCR and cell biology methods. When we compared protein levels according to HF etiology, nucleolin was increased in both ICM (117%, p< 0.05) and DCM (141%, p< 0.01). Moreover, mRNA expression were also upregulated in ICM (1.46-fold, p< 0.05) and DCM (1.70-fold, p< 0.05. Immunofluorescence studies showed that the highest intensity of nucleolin was into nucleolus (p< 0.0001), and it was increased in pathological hearts (p< 0.0001). Ultrastructure analysis by electron microscopy showed an increase in the nucleus and nucleolus size in ICM (17%, p< 0.05 and 131%, p< 0.001) and DCM (56%, p< 0.01 and 69%, p< 0.01). Nucleolar organization was influenced by HF irrespective of etiology, increasing fibrillar centers (p< 0.001), perinucleolar chromatin (p< 0.01) and dense fibrillar components (p< 0.01). Finally, left ventricular function parameters were related with nucleolin levels in ischemic hearts (p< 0.0001). The present study demonstrates that HF influences on morphology and organization of nucleolar components, revealing changes in the expression and in the levels of nucleolin protein. © 2012 Elsevier Inc. | es_ES |
dc.description.sponsorship | This work was supported by grants from the National Institute of Health "Fondo de Investigaciones Sanitarias of Institut de Salud Carlos III" [REDINSCOR 06/0003/1001, Project PI07/0462]. | en_EN |
dc.language | Inglés | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.ispartof | Biochemical and Biophysical Research Communications | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Heart failure | es_ES |
dc.subject | Nucleolin | es_ES |
dc.subject | Nucleolus | es_ES |
dc.subject | Messenger RNA | es_ES |
dc.subject | Adult | es_ES |
dc.subject | Article | es_ES |
dc.subject | Cell nucleus | es_ES |
dc.subject | Cell size | es_ES |
dc.subject | Chromatin | es_ES |
dc.subject | Congestive cardiomyopathy | es_ES |
dc.subject | Controlled study | es_ES |
dc.subject | Female | es_ES |
dc.subject | Heart left ventricle function | es_ES |
dc.subject | Heart muscle cell | es_ES |
dc.subject | Heart transplantation | es_ES |
dc.subject | Human | es_ES |
dc.subject | Human cell | es_ES |
dc.subject | Human tissue | es_ES |
dc.subject | Immunofluorescence | es_ES |
dc.subject | Ischemic cardiomyopathy | es_ES |
dc.subject | Major clinical study | es_ES |
dc.subject | Male | es_ES |
dc.subject | Nucleolar localization signal | es_ES |
dc.subject | Priority journal | es_ES |
dc.subject | Protein expression | es_ES |
dc.subject | Reverse transcription polymerase chain reaction | es_ES |
dc.subject | Surgical patient | es_ES |
dc.subject | Ultrastructure analysis and electron microscopy | es_ES |
dc.subject | Upregulation | es_ES |
dc.subject | Western blotting | es_ES |
dc.subject.classification | MICROBIOLOGIA | es_ES |
dc.title | Influence of heart failure on nucleolar organization and protein expression in human hearts | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/j.bbrc.2011.12.151 | |
dc.relation.projectID | info:eu-repo/grantAgreement/ISCIII//PI07%2F0462/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ISCIII//06%2F0003%2F1001/ | es_ES |
dc.rights.accessRights | Cerrado | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia | es_ES |
dc.description.bibliographicCitation | Rosello Lleti, E.; Rivera, M.; Cortés, R.; Azorin, I.; Sirera Pérez, R.; Martínez Dolz, L.; Hove, L.... (2012). Influence of heart failure on nucleolar organization and protein expression in human hearts. Biochemical and Biophysical Research Communications. 418(2):222-228. https://doi.org/10.1016/j.bbrc.2011.12.151 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://dx.doi.org/10.1016/j.bbrc.2011.12.151 | es_ES |
dc.description.upvformatpinicio | 222 | es_ES |
dc.description.upvformatpfin | 228 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 418 | es_ES |
dc.description.issue | 2 | es_ES |
dc.relation.senia | 231614 | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |