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Influence of heart failure on nucleolar organization and protein expression in human hearts

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Influence of heart failure on nucleolar organization and protein expression in human hearts

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dc.contributor.author Rosello Lleti, Esther es_ES
dc.contributor.author Rivera, Miguel es_ES
dc.contributor.author Cortés, Raquel es_ES
dc.contributor.author Azorin, Inmaculada es_ES
dc.contributor.author Sirera Pérez, Rafael es_ES
dc.contributor.author Martínez Dolz, Luis es_ES
dc.contributor.author Hove, Leif es_ES
dc.contributor.author Cinca, Juan es_ES
dc.contributor.author Lago, Francisca es_ES
dc.contributor.author González Juanatey, José R. es_ES
dc.contributor.author Salvador, Antonio es_ES
dc.contributor.author Portolés, Manuel es_ES
dc.date.accessioned 2016-06-01T10:00:21Z
dc.date.available 2016-06-01T10:00:21Z
dc.date.issued 2012-02-10
dc.identifier.issn 0006-291X
dc.identifier.uri http://hdl.handle.net/10251/65039
dc.description.abstract [EN] We investigate for the first time the influence of heart failure (HF) on nucleolar organization and proteins in patients with ischemic (ICM) or dilated cardiomyopathy (DCM). A total of 71 human hearts from ICM (n= 38) and DCM (n= 27) patients, undergoing heart transplantation and control donors (n= 6), were analysed by western-blotting, RT-PCR and cell biology methods. When we compared protein levels according to HF etiology, nucleolin was increased in both ICM (117%, p< 0.05) and DCM (141%, p< 0.01). Moreover, mRNA expression were also upregulated in ICM (1.46-fold, p< 0.05) and DCM (1.70-fold, p< 0.05. Immunofluorescence studies showed that the highest intensity of nucleolin was into nucleolus (p< 0.0001), and it was increased in pathological hearts (p< 0.0001). Ultrastructure analysis by electron microscopy showed an increase in the nucleus and nucleolus size in ICM (17%, p< 0.05 and 131%, p< 0.001) and DCM (56%, p< 0.01 and 69%, p< 0.01). Nucleolar organization was influenced by HF irrespective of etiology, increasing fibrillar centers (p< 0.001), perinucleolar chromatin (p< 0.01) and dense fibrillar components (p< 0.01). Finally, left ventricular function parameters were related with nucleolin levels in ischemic hearts (p< 0.0001). The present study demonstrates that HF influences on morphology and organization of nucleolar components, revealing changes in the expression and in the levels of nucleolin protein. © 2012 Elsevier Inc. es_ES
dc.description.sponsorship This work was supported by grants from the National Institute of Health "Fondo de Investigaciones Sanitarias of Institut de Salud Carlos III" [REDINSCOR 06/0003/1001, Project PI07/0462]. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Biochemical and Biophysical Research Communications es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Heart failure es_ES
dc.subject Nucleolin es_ES
dc.subject Nucleolus es_ES
dc.subject Messenger RNA es_ES
dc.subject Adult es_ES
dc.subject Article es_ES
dc.subject Cell nucleus es_ES
dc.subject Cell size es_ES
dc.subject Chromatin es_ES
dc.subject Congestive cardiomyopathy es_ES
dc.subject Controlled study es_ES
dc.subject Female es_ES
dc.subject Heart left ventricle function es_ES
dc.subject Heart muscle cell es_ES
dc.subject Heart transplantation es_ES
dc.subject Human es_ES
dc.subject Human cell es_ES
dc.subject Human tissue es_ES
dc.subject Immunofluorescence es_ES
dc.subject Ischemic cardiomyopathy es_ES
dc.subject Major clinical study es_ES
dc.subject Male es_ES
dc.subject Nucleolar localization signal es_ES
dc.subject Priority journal es_ES
dc.subject Protein expression es_ES
dc.subject Reverse transcription polymerase chain reaction es_ES
dc.subject Surgical patient es_ES
dc.subject Ultrastructure analysis and electron microscopy es_ES
dc.subject Upregulation es_ES
dc.subject Western blotting es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.title Influence of heart failure on nucleolar organization and protein expression in human hearts es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.bbrc.2011.12.151
dc.relation.projectID info:eu-repo/grantAgreement/ISCIII//PI07%2F0462/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ISCIII//06%2F0003%2F1001/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Rosello Lleti, E.; Rivera, M.; Cortés, R.; Azorin, I.; Sirera Pérez, R.; Martínez Dolz, L.; Hove, L.... (2012). Influence of heart failure on nucleolar organization and protein expression in human hearts. Biochemical and Biophysical Research Communications. 418(2):222-228. https://doi.org/10.1016/j.bbrc.2011.12.151 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1016/j.bbrc.2011.12.151 es_ES
dc.description.upvformatpinicio 222 es_ES
dc.description.upvformatpfin 228 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 418 es_ES
dc.description.issue 2 es_ES
dc.relation.senia 231614 es_ES
dc.contributor.funder Instituto de Salud Carlos III es_ES


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