Identification of Viable Helicobacter pylori in Drinking Water Supplies by Cultural and Molecular Techniques

Abstract

BackgroundHelicobacter pylori is one of the most common causes of chronic bacterial infection in humans, directly related to peptic ulcer and gastric cancer. It has been suggested that H.pylori can be acquired through different transmission routes, including water. In this study, culture and qPCR were used to detect and identify the presence of H.pylori in drinking water. Furthermore, the combined techniques PMA-qPCR and DVC-FISH were applied for detection of viable cells of H.pylori.

ResultsAmong 24 drinking water samples, 16 samples were positive for the presence of H.pylori, but viable cells were only detected in six samples. Characteristic colonies, covered by a mass of bacterial unspecific growth, were observed on selective agar plates from an only sample, after enrichment. The mixed culture was submitted to DVC-FISH and qPCR analysis, followed by sequencing of the amplicons. Molecular techniques confirmed the growth of H.pylori on the agar plate.

ConclusionsOur results demonstrate for the first time that H.pylori can survive and be potentially infective in drinking water, showing that water distribution systems could be a potential route for H.pylori transmission.