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Development of a three-dimensional cell culture system based on microfluidics for nuclear magnetic resonance and optical monitoring

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Development of a three-dimensional cell culture system based on microfluidics for nuclear magnetic resonance and optical monitoring

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dc.contributor.author Esteve, Vicent es_ES
dc.contributor.author Berganzo, Javier es_ES
dc.contributor.author Monge, Rosa es_ES
dc.contributor.author Martínez-Bisbal, M.Carmen es_ES
dc.contributor.author Villa, Rosa es_ES
dc.contributor.author Celda, Bernardo es_ES
dc.contributor.author Fernández, Luis es_ES
dc.date.accessioned 2016-06-06T11:28:56Z
dc.date.available 2016-06-06T11:28:56Z
dc.date.issued 2014-11
dc.identifier.issn 1932-1058
dc.identifier.uri http://hdl.handle.net/10251/65306
dc.description.abstract A new microfluidic cell culture device compatible with real-time nuclear magnetic resonance (NMR) is presented here. The intended application is the long-term monitoring of 3D cell cultures by several techniques. The system has been designed to fit inside commercially available NMR equipment to obtain maximum readout resolution when working with small samples. Moreover, the microfluidic device integrates a fibre-optic-based sensor to monitor parameters such as oxygen, pH, or temperature during NMR monitoring, and it also allows the use of optical microscopy techniques such as confocal fluorescence microscopy. This manuscript reports the initial trials culturing neurospheres inside the microchamber of this device and the preliminary images and spatially localised spectra obtained by NMR. The images show the presence of a necrotic area in the interior of the neurospheres, as is frequently observed in histological preparations; this phenomenon appears whenever the distance between the cells and fresh nutrients impairs the diffusion of oxygen. Moreover, the spectra acquired in a volume of 8 nl inside the neurosphere show an accumulation of lactate and lipids, which are indicative of anoxic conditions. Additionally, a basis for general temperature control and monitoring and a graphical control software have been developed and are also described. The complete platform will allow biomedical assays of therapeutic agents to be performed in the early phases of therapeutic development. Thus, small quantities of drugs or advanced nanodevices may be studied long-term under simulated living conditions that mimic the flow and distribution of nutrients. (C) 2014 AIP Publishing LLC. es_ES
dc.description.sponsorship This work was supported partially by the Basque Government under the Etortek-Microsystems Programme, the ERANET-Neuron Project EPINet (EUI2009-04093) and SAF2009-14724-C02-02 from the Spanish Ministry of Science and Innovation and the European Regional Development Fund. The authors would like to thank Jorge Elizalde (Ikerlan S. Coop.) for their help and support and CIBER-BBN for general funding and support of the project. en_EN
dc.language Inglés es_ES
dc.publisher American Institute of Physics (AIP) es_ES
dc.relation Basque Government under the Etortek-Microsystems Programme es_ES
dc.relation info:eu-repo/grantAgreement/MICINN//EUI2009-04093/ES/EPINET: UNDERSTANDING AND MANIPULATING EPILEPTIC NETWORKS/ es_ES
dc.relation info:eu-repo/grantAgreement/MICINN//SAF2009-14724-C02-02/ES/Desarrollo De Un Nuevo Metodo De Diagnostico No Invasivo De Patologias Corneales Por Bioimpedancia Utilizando Micronanoelectrodos/ es_ES
dc.relation European Regional Development Fund es_ES
dc.relation CIBER-BBN es_ES
dc.relation.ispartof Biomicrofluidics es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject On-a-chip es_ES
dc.subject Gold nanoparticles es_ES
dc.subject NMR-SPECTROSCOPY es_ES
dc.subject Mammalian-Cells es_ES
dc.subject Single neurons es_ES
dc.subject MR Microscopy es_ES
dc.subject In-vivo es_ES
dc.subject Microstructure es_ES
dc.subject Platforms es_ES
dc.subject Perfusion es_ES
dc.title Development of a three-dimensional cell culture system based on microfluidics for nuclear magnetic resonance and optical monitoring es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1063/1.4902002
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic es_ES
dc.description.bibliographicCitation Esteve, V.; Berganzo, J.; Monge, R.; Martínez-Bisbal, M.; Villa, R.; Celda, B.; Fernández, L. (2014). Development of a three-dimensional cell culture system based on microfluidics for nuclear magnetic resonance and optical monitoring. Biomicrofluidics. 8(6):064105-1-064105-11. https://doi.org/10.1063/1.4902002 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1063/1.4902002 es_ES
dc.description.upvformatpinicio 064105-1 es_ES
dc.description.upvformatpfin 064105-11 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 8 es_ES
dc.description.issue 6 es_ES
dc.relation.senia 276781 es_ES
dc.identifier.pmcid PMC4240776 en_EN


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