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Genetic alterations leading to increases in internal potassium concentrations are detrimental for DNA integrity in Saccharomyces cerevisiae

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Genetic alterations leading to increases in internal potassium concentrations are detrimental for DNA integrity in Saccharomyces cerevisiae

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dc.contributor.author Merchan, Stéphanie Emilie es_ES
dc.contributor.author Pedelini, Leda es_ES
dc.contributor.author Hueso Lorente, Guillem es_ES
dc.contributor.author Calzada, Arturo es_ES
dc.contributor.author Serrano Salom, Ramón es_ES
dc.contributor.author Yenush, Lynne es_ES
dc.date.accessioned 2016-07-11T08:02:55Z
dc.date.available 2016-07-11T08:02:55Z
dc.date.issued 2011-02
dc.identifier.issn 1356-9597
dc.identifier.uri http://hdl.handle.net/10251/67397
dc.description.abstract [EN] We have investigated the effects of alterations in potassium homeostasis on cell cycle progression and genome stability in Saccharomyces cerevisiae. Yeast strains lacking the PPZ1 and PPZ2 phosphatase genes, which aberrantly accumulate potassium, are sensitive to agents causing replicative stress or DNA damage and present a cell cycle delay in the G(1)/S phase. A synthetic slow growth phenotype was identified in a subset of DNA repair mutants upon inhibition of Ppz activity. Moreover, we observe that this slow growth phenotype observed in cdc7ts mutants with reduced Ppz activity is reverted by disrupting the TRK1 potassium transporter gene. As over-expression of a mammalian potassium transporter leads to similar phenotypes, we conclude that these defects can be attributed to potassium accumulation. As we reported previously, internal potassium accumulation activates the Slt2 MAP kinase pathway. We show that the removal of SLT2 in ppz1 ppz2 mutants ameliorates sensitivity to agents causing replication stress and DNA damage, whereas over-activation of the pathway leads to similar cell cycle-related defects. Taken together, these results are consistent with inappropriate potassium accumulation reducing DNA replication efficiency, negatively influencing DNA integrity and leading to the requirement of mismatch repair, the MRX complex, or homologous recombination pathways for normal growth. es_ES
dc.description.sponsorship This work was supported by the Ministry of Education and Science (Madrid) [grant number BFU2005-06388-C04-01/BMC]; the Ministry of Science and Innovation (Madrid) [grant number BFU2008-04188-C03-02/BMC]; and Spanish Ministry of Science and Technology [Ramon y Cajal fellowship to L.Y. and predoctoral fellowship to S.M.]. The authors would like to acknowledge Jose Ramon Murguia for helpful comments and Jose Miguel Mulet, David Quintana, Maria Molina, and Jost Ludwig for providing strains and plasmids.
dc.language Inglés es_ES
dc.publisher Wiley es_ES
dc.relation.ispartof Genes to Cells es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject PPZ PROTEIN PHOSPHATASES es_ES
dc.subject POLARIZED CELL-GROWTH es_ES
dc.subject MAP KINASE PATHWAY es_ES
dc.subject SALT TOLERANCE es_ES
dc.subject ALPHA-AMINOADIPATE es_ES
dc.subject CATION-TRANSPORT es_ES
dc.subject INTRACELLULAR PH es_ES
dc.subject CYCLE REGULATOR es_ES
dc.subject MKIR2.1 CHANNEL es_ES
dc.subject WALL INTEGRITY es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Genetic alterations leading to increases in internal potassium concentrations are detrimental for DNA integrity in Saccharomyces cerevisiae es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/j.1365-2443.2010.01472.x
dc.relation.projectID info:eu-repo/grantAgreement/MEC//BFU2005-06388-C04-01/ES/REGULACION DEL POTASIO Y PH INTRACELULAR EN LEVADURA Y ARABIDOPSIS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BFU2008-04188-C03-02/ES/RUTAS DE TRANSDUCCION DE SEÑALES EN LA REGULACION DE LA HOMEOSTASIS IONICA/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Merchan, SE.; Pedelini, L.; Hueso Lorente, G.; Calzada, A.; Serrano Salom, R.; Yenush, L. (2011). Genetic alterations leading to increases in internal potassium concentrations are detrimental for DNA integrity in Saccharomyces cerevisiae. Genes to Cells. 16(2):152-165. https://doi.org/10.1111/j.1365-2443.2010.01472.x es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1111/j.1365-2443.2010.01472.x es_ES
dc.description.upvformatpinicio 152 es_ES
dc.description.upvformatpfin 165 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 16 es_ES
dc.description.issue 2 es_ES
dc.relation.senia 39416 es_ES
dc.identifier.pmid 21143561
dc.contributor.funder Ministerio de Educación y Ciencia
dc.contributor.funder Ministerio de Ciencia e Innovación
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