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Multiresidue determination of antibiotics in feed and fish samples for food safety evaluation. Comparison of immunoassay vs LC-MS-MS

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Multiresidue determination of antibiotics in feed and fish samples for food safety evaluation. Comparison of immunoassay vs LC-MS-MS

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dc.contributor.author Cháfer Pericás, María Consuelo es_ES
dc.contributor.author Maquieira Catala, Ángel es_ES
dc.contributor.author Puchades, Rosa es_ES
dc.contributor.author Miralles, Javier es_ES
dc.contributor.author Moreno, Amelia es_ES
dc.date.accessioned 2016-07-14T12:45:45Z
dc.date.available 2016-07-14T12:45:45Z
dc.date.issued 2011-06
dc.identifier.issn 0956-7135
dc.identifier.uri http://hdl.handle.net/10251/67605
dc.description.abstract [EN] Antibiotic residues (sulfonamides and tetracyclines) were determined in Gilthead sea bream (Sparus aurata) and feed samples by means of immunoassays and LC-MS-MS (liquid chromatography-mass spectrometry 2). A preliminary study to know the withdrawal time of oxytetracycline in Gilthead sea bream samples was also conducted. It was carried out using immunoassays based on photometric detection of horseradish peroxidase (HRP) activity and time-resolved fluorometric detection of coproporphyrin of Platinum (II) (ELISA and TR-FIA, respectively). The results were compared to those obtained using an LC-MS-MS methodology. They showed that approximately 37 days would be the withdrawal time in order not to exceed the MRL and fish could be commercialized with safety. Regarding feed samples analysis, an LC-MS-MS method was optimized including sample treatment. Average recoveries (n = 6) ranging from 78 to 108% were obtained and precision of the method was between 0.2 and 3%. The same sample treatment was applied to the feed immunoanalysis obtaining satisfactory results.Finally, 20 fish and 4 feed samples were analysed in order to confirm the feasibility of the immunoassays for detecting antibiotic residues. Sulfonamide residues were not detected in any fish sample. Tetracycline residues were detected in some fish samples from marine farms, with total concentrations between 2.1 and 152 ng g -1. In all cases, the obtained results correlated well with those achieved by LC-MS-MS. Therefore, the new methodology allows for food safety of the medicated fish. © 2010 Elsevier Ltd es_ES
dc.description.sponsorship The authors are grateful to the Spanish Ministerio de Ciencia e Innovacion (Project PET 2006-009-00) and to Generalitat Valenciana (ACOMP/2009/236) for financial support received. en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation Spanish Ministerio de Ciencia e Innovacion [PET 2006-009-00] es_ES
dc.relation Generalitat Valenciana [ACOMP/2009/236] es_ES
dc.relation.ispartof Food Control es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Antibiotic es_ES
dc.subject ELISA es_ES
dc.subject Feed es_ES
dc.subject Fish es_ES
dc.subject LC-MS-MS es_ES
dc.subject Archosargus rhomboidalis es_ES
dc.subject Armoracia rusticana es_ES
dc.subject Sparus aurata es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Multiresidue determination of antibiotics in feed and fish samples for food safety evaluation. Comparison of immunoassay vs LC-MS-MS es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.foodcont.2010.12.008
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic es_ES
dc.description.bibliographicCitation Cháfer Pericás, MC.; Maquieira Catala, Á.; Puchades, R.; Miralles, J.; Moreno, A. (2011). Multiresidue determination of antibiotics in feed and fish samples for food safety evaluation. Comparison of immunoassay vs LC-MS-MS. Food Control. 22(6):993-999. doi:10.1016/j.foodcont.2010.12.008 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion https://dx.doi.org/10.1016/j.foodcont.2010.12.008 es_ES
dc.description.upvformatpinicio 993 es_ES
dc.description.upvformatpfin 999 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 22 es_ES
dc.description.issue 6 es_ES
dc.relation.senia 212781 es_ES


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