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Combinatorial Analysis of Secretory Immunoglobulin A (sIgA) Expression in Plants

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Combinatorial Analysis of Secretory Immunoglobulin A (sIgA) Expression in Plants

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dc.contributor.author Juárez Ortega, Paloma es_ES
dc.contributor.author Huet Trujillo, Estefanía es_ES
dc.contributor.author Sarrion-Perdigones, Alejandro es_ES
dc.contributor.author Falconi, E.E. es_ES
dc.contributor.author Granell Richart, Antonio es_ES
dc.contributor.author Orzáez Calatayud, Diego Vicente es_ES
dc.date.accessioned 2016-07-18T07:23:50Z
dc.date.available 2016-07-18T07:23:50Z
dc.date.issued 2013-03
dc.identifier.issn 1422-0067
dc.identifier.uri http://hdl.handle.net/10251/67693
dc.description.abstract Delivery of secretory immunoglobulin A (sIgA) to mucosal surfaces as a passive immunotherapy agent is a promising strategy to prevent infectious diseases. Recombinant sIgA production in plants requires the co-expression of four transcriptional units encoding the light chain (LC), heavy chain (HC), joining chain (JC) and secretory component (SC). As a way to optimize sIgA production in plants, we tested the combinatorial expression of 16 versions of a human sIgA against the VP8* rotavirus antigen in Nicotiana benthamiana, using the recently developed GoldenBraid multigene assembly system. Each sIgA version was obtained by combining one of the two types of HC (alpha 1 and alpha 2) with one of the two LC types (k and lambda) and linking or not a KDEL peptide to the HC and/or SC. From the analysis of the anti-VP8* activity, it was concluded that those sIgA versions carrying HC alpha 1 and LC lambda provided the highest yields. Moreover, ER retention significantly increased antibody production, particularly when the KDEL signal was linked to the SC. Maximum expression levels of 32.5 mu g IgA/g fresh weight (FW) were obtained in the best performing combination, with an estimated 33% of it in the form of a secretory complex. es_ES
dc.description.sponsorship This work has been funded by Grant BIO2010-15384 from Plan Nacional I + D of the Spanish Ministry of Science. Juarez P. is a recipient of a FPU fellowship, and Sarrion-Perdigones A. and Huet-Trujillo E. are recipients of a FPI fellowship. We want to thank Monedero for kindly providing scFv and VP8* clones. en_EN
dc.language Inglés es_ES
dc.publisher MDPI es_ES
dc.relation Plan Nacional I + D of the Spanish Ministry of Science BIO2010-15384 es_ES
dc.relation FPU es_ES
dc.relation FPI es_ES
dc.relation.ispartof International Journal of Molecular Sciences es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Secretory IgA es_ES
dc.subject Antibody es_ES
dc.subject Rotavirus es_ES
dc.subject GoldenBraid es_ES
dc.subject Plant synthetic biology es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Combinatorial Analysis of Secretory Immunoglobulin A (sIgA) Expression in Plants es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/ijms14036205
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Juárez Ortega, P.; Huet Trujillo, E.; Sarrion-Perdigones, A.; Falconi, E.; Granell Richart, A.; Orzáez Calatayud, DV. (2013). Combinatorial Analysis of Secretory Immunoglobulin A (sIgA) Expression in Plants. International Journal of Molecular Sciences. 14(3):6205-6222. doi:10.3390/ijms14036205 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.3390/ijms14036205 es_ES
dc.description.upvformatpinicio 6205 es_ES
dc.description.upvformatpfin 6222 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 14 es_ES
dc.description.issue 3 es_ES
dc.relation.senia 248407 es_ES
dc.identifier.pmid 23507755 en_EN
dc.identifier.pmcid PMC3634489 en_EN


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