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Coordinated gene regulation in the initial phase of salt stress adaptation

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Coordinated gene regulation in the initial phase of salt stress adaptation

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dc.contributor.author Vanacloig Pedros, Mª ELENA es_ES
dc.contributor.author Bets Plasencia, Carolina es_ES
dc.contributor.author Pascual-Ahuir Giner, María Desamparados es_ES
dc.contributor.author Proft, Markus Hans es_ES
dc.date.accessioned 2016-07-20T07:46:21Z
dc.date.available 2016-07-20T07:46:21Z
dc.date.issued 2015-04-17
dc.identifier.issn 0021-9258
dc.identifier.uri http://hdl.handle.net/10251/67885
dc.description This research was originally published in Journal of Biological Chemistry, 2015 - 16 : 10175- 10163 © the American Society for Biochemistry and Molecular Biology
dc.description.abstract [EN] Stress triggers complex transcriptional responses, which include both gene activation and repression. We used time-resolved reporter assays in living yeast cells to gain insights into the coordination of positive and negative control of gene expression upon salt stress. We found that the repression of housekeeping genes coincides with the transient activation of defense genes and that the timing of this expression pattern depends on the severity of the stress. Moreover, we identified mutants that caused an alteration in the kinetics of this transcriptional control. Loss of function of the vacuolar H+-ATPase (vma1) or a defect in the biosynthesis of the osmolyte glycerol (gpd1) caused a prolonged repression of housekeeping genes and a delay in gene activation at inducible loci. Both mutants have a defect in the relocation of RNA polymerase II complexes at stress defense genes. Accordingly salt-activated transcription is delayed and less efficient upon partially respiratory growth conditions in which glycerol production is significantly reduced. Furthermore, the loss of Hog1 MAP kinase function aggravates the loss of RNA polymerase II from housekeeping loci, which apparently do not accumulate at inducible genes. Additionally the Def1 RNA polymerase II degradation factor, but not a high pool of nuclear polymerase II complexes, is needed for efficient stress-induced gene activation. The data presented here indicate that the finely tuned transcriptional control upon salt stress is dependent on physiological functions of the cell, such as the intracellular ion balance, the protective accumulation of osmolyte molecules, and the RNA polymerase II turnover. es_ES
dc.description.sponsorship This work was supported by Ministerio de Economia y Competitividad Grant BFU2011-23326 (to M. P.). en_EN
dc.language Inglés es_ES
dc.publisher American Society for Biochemistry and Molecular Biology es_ES
dc.relation Ministerio de Economia y Competitividad BFU2011-23326 es_ES
dc.relation.ispartof Journal of Biological Chemistry es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Gene Expression es_ES
dc.subject Glycerol es_ES
dc.subject Hog1 es_ES
dc.subject Osmotic Stress es_ES
dc.subject Saccharomyces cerevisiae es_ES
dc.subject Stress Response es_ES
dc.subject Transcription Regulation es_ES
dc.subject Vacuolar ATPase es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Coordinated gene regulation in the initial phase of salt stress adaptation es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1074/jbc.M115.637264
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Vanacloig Pedros, ME.; Bets Plasencia, C.; Pascual-Ahuir Giner, MD.; Proft, MH. (2015). Coordinated gene regulation in the initial phase of salt stress adaptation. Journal of Biological Chemistry. 16(290):10163-10175. doi:10.1074/jbc.M115.637264 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion https://dx.doi.org/10.1074/jbc.M115.637264 es_ES
dc.description.upvformatpinicio 10163 es_ES
dc.description.upvformatpfin 10175 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 16 es_ES
dc.description.issue 290 es_ES
dc.relation.senia 290529 es_ES
dc.identifier.eissn 1083-351X
dc.identifier.pmcid PMC4400332 en_EN
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