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Histology of Quercus ilex fine roots during infection by Phytophthora cinnamomi

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Histology of Quercus ilex fine roots during infection by Phytophthora cinnamomi

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dc.contributor.author Redondo, Miguel Ángel es_ES
dc.contributor.author Pérez Sierra, Ana María es_ES
dc.contributor.author Abad Campos, Paloma es_ES
dc.contributor.author Torres, Lilian es_ES
dc.contributor.author Solla, Alejandro es_ES
dc.contributor.author Reig Armiñana, José es_ES
dc.contributor.author García-Breijo, Francisco-José es_ES
dc.date.accessioned 2016-07-26T06:53:21Z
dc.date.available 2016-07-26T06:53:21Z
dc.date.issued 2015-12
dc.identifier.issn 0931-1890
dc.identifier.uri http://hdl.handle.net/10251/68170
dc.description.abstract [EN] This study aimed to elucidate the infection process of the invasive pathogen Phytophthora cinnamomi on primary and secondary roots of 2-month-old Quercus ilex seedlings. To test if different methods of inoculation lead to different changes in the host caused by the pathogen, the root system of plants was either immersed into a suspension of P. cinnamomi zoospores, or placed in direct contact with agar plugs colonized by P. cinnamomi mycelium. Histology of root sections obtained every 24 h for 10 days revealed similar changes in the structure of cells and tissues of the host irrespective of the inoculation method used. However, the immersion method resulted in a delay in the colonization of the host, different aerial symptoms, and the formation of different reproductive structures of the pathogen. Emerging secondary and tertiary roots and sites where secondary or tertiary roots were about to emerge were identified as main entry points. Hyphae in the xylem tissues were more frequently found in secondary than in primary roots, but in both types of roots the phloem was the most important pathway of colonization. For the first time in the interaction between Q. ilex and P. cinnamomi, transmission electron microscopy was used to describe degradation of the host cell walls, pit penetration and extrahaustorial matrix. Haustoria development during intracellular growth and hyphal aggregations (stromata) caused no damage to the host cell walls indicating hemibiotrophic parasitism es_ES
dc.description.sponsorship This research was financially supported by the Project AGL2011-30438, by the Vicerrectorado de Investigacion from the Polytechnic University of Valencia, and by the "Julio Iranzo'' laboratory from the Botanic Garden of Valencia. The authors deeply appreciate the help of the staff from both institutions, particularly the valuable contribution of Nuria Cebrian Gomez. Additionally, the staff from the microscopy sections from Polytechnic University of Valencia and University of Valencia have provided us with valuable help. We are grateful to the two anonymous reviewers for the valuable comments in an earlier version of this manuscript. en_EN
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation Vicerrectorado de Investigacion from the Polytechnic University of Valencia AGL2011-30438 es_ES
dc.relation "Julio Iranzo'' laboratory from the Botanic Garden of Valencia es_ES
dc.relation.ispartof Trees - Structure and Function es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Cell structure es_ES
dc.subject Histological alterations es_ES
dc.subject Histopathology es_ES
dc.subject Microscopy es_ES
dc.subject Pathogenesis es_ES
dc.subject Invasive pathogen es_ES
dc.subject.classification BOTANICA es_ES
dc.subject.classification PRODUCCION VEGETAL es_ES
dc.subject.classification BIOLOGIA VEGETAL es_ES
dc.title Histology of Quercus ilex fine roots during infection by Phytophthora cinnamomi es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s00468-015-1275-3
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Redondo, MÁ.; Pérez Sierra, AM.; Abad Campos, P.; Torres, L.; Solla, A.; Reig Armiñana, J.; García-Breijo, F. (2015). Histology of Quercus ilex fine roots during infection by Phytophthora cinnamomi. Trees - Structure and Function. 29(6):1943-1957. doi:10.1007/s00468-015-1275-3 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion https://dx.doi.org/10.1007/s00468-015-1275-3 es_ES
dc.description.upvformatpinicio 1943 es_ES
dc.description.upvformatpfin 1957 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 29 es_ES
dc.description.issue 6 es_ES
dc.relation.senia 292853 es_ES
dc.identifier.eissn 1432-2285
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