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Transgenic tomato plants overexpressing tyramine N-hydroxycinnamoyltransferase exhibit elevated hydroxycinnamic acid amide levels and enhanced resistance to Pseudomonas syringae

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Transgenic tomato plants overexpressing tyramine N-hydroxycinnamoyltransferase exhibit elevated hydroxycinnamic acid amide levels and enhanced resistance to Pseudomonas syringae

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dc.contributor.author Campos Beneyto, Laura es_ES
dc.contributor.author Lisón Párraga, María Purificación es_ES
dc.contributor.author López Gresa, Mª Pilar es_ES
dc.contributor.author Rodrigo Bravo, Ismael es_ES
dc.contributor.author Zacarés Sanmartín, Laura es_ES
dc.contributor.author Conejero Tomás, Vicente es_ES
dc.contributor.author Belles Albert, José Mª
dc.date.accessioned 2016-07-28T11:40:53Z
dc.date.available 2016-07-28T11:40:53Z
dc.date.issued 2014-10
dc.identifier.issn 0894-0282
dc.identifier.uri http://hdl.handle.net/10251/68393
dc.description This paper has not yet been peer reviewed, copyedited or proofread. The final published version may differ. The final version of the article is available at: http://dx.doi.org/10.1094/MPMI-04-14-0104-R © 2014 The American Phytopathological Society es_ES
dc.description.abstract [EN] Hydroxycinnamic acid amides (HCAA) are secondary metabolites involved in plant development and defense that have been widely reported throughout the plant kingdom. These phenolics show antioxidant, antiviral, antibacterial, and antifungal activities. Hydroxycinnamoyl-CoA: tyramine N-hydroxycinnamoyl transferase (THT) is the key enzyme in HCAA synthesis and is induced in response to pathogen infection, wounding, or elicitor treatments, preceding HCAA accumulation. We have engineered transgenic tomato plants overexpressing tomato THT. These plants displayed an enhanced THT gene expression in leaves as compared with wild type (WT) plants. Consequently, leaves of THT-over-expressing plants showed a higher constitutive accumulation of the amide coumaroyltyramine (CT). Similar results were found in flowers and fruits. Moreover, feruloyltyramine (FT) also accumulated in these tissues, being present at higher levels in transgenic plants. Accumulation of CT, FT and octopamine, and noradrenaline HCAA in response to Pseudomonas syringae pv. tomato infection was higher in transgenic plants than in the WT plants. Transgenic plants showed an enhanced resistance to the bacterial infection. In addition, this HCAA accumulation was accompanied by an increase in salicylic acid levels and pathogenesis-related gene induction. Taken together, these results suggest that HCAA may play an important role in the defense of tomato plants against P. syringae infection. es_ES
dc.description.sponsorship The authors are grateful to C. Torres, A. Sauri, and T. Caballero for technical support. We also thank C. Gomez-Mena for her assistance in tomato flower sampling. This work was supported by Grant BIO2012-33419 from the Direccion General de Programas y Transferencia de Conocimiento, from the Spanish Ministry of Science and Innovation. L. Campos was the recipient of a predoctoral fellowship ACIF/2010/231 from Generalitat Valenciana (Spain). The work presented here was carried out in collaboration between all authors. J. M. Belles defined the research theme. L. Campos, L. Zacares and M. P. Lopez-Gresa carried out the laboratory experiments. P. Lison and I. Rodrigo contributed to the experimental design and the interpretation of the data. L. Campos drafted the article. M. P. Lopez-Gresa, P. Lison and I. Rodrigo participated in revising it critically for important intellectual content. J. M. Belles and V. Conejero gave the final approval of the version to be published. Each author has participated sufficiently in the work to take public responsibility for the content. en_EN
dc.language Inglés es_ES
dc.publisher American Phytopathological Society es_ES
dc.relation Direccion General de Programas y Transferencia de Conocimiento BIO2012-33419 es_ES
dc.relation Spanish Ministry of Science and Innovation es_ES
dc.relation Generalitat Valenciana (Spain) ACIF/2010/231 es_ES
dc.relation.ispartof Molecular Plant-Microbe Interactions es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject CELL-SUSPENSION-CULTURES es_ES
dc.subject CARTHAMUS-TINCTORIUS L. es_ES
dc.subject SOLANUM-TUBEROSUM es_ES
dc.subject SALICYLIC-ACID es_ES
dc.subject HYPERSENSITIVE RESPONSE es_ES
dc.subject GENTISIC ACID es_ES
dc.subject XANTHOMONAS-CAMPESTRIS es_ES
dc.subject DISEASE RESISTANCE es_ES
dc.subject NICOTIANA-TABACUM es_ES
dc.subject TOBACCO-LEAVES es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Transgenic tomato plants overexpressing tyramine N-hydroxycinnamoyltransferase exhibit elevated hydroxycinnamic acid amide levels and enhanced resistance to Pseudomonas syringae es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1094/MPMI-04-14-0104-R
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Campos Beneyto, L.; Lisón Párraga, MP.; López Gresa, MP.; Rodrigo Bravo, I.; Zacarés Sanmartín, L.; Conejero Tomás, V.; Belles Albert, JM. (2014). Transgenic tomato plants overexpressing tyramine N-hydroxycinnamoyltransferase exhibit elevated hydroxycinnamic acid amide levels and enhanced resistance to Pseudomonas syringae. Molecular Plant-Microbe Interactions. 27(10):1159-1169. doi:10.1094/MPMI-04-14-0104-R es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion https://dx.doi.org/10.1094/MPMI-04-14-0104-R es_ES
dc.description.upvformatpinicio 1159 es_ES
dc.description.upvformatpfin 1169 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 27 es_ES
dc.description.issue 10 es_ES
dc.relation.senia 272036 es_ES
dc.identifier.eissn 1943-7706


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