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Parallel solid-phase isothermal amplification and detection of multiple DNA targets in microliter-sized wells of a digital versatile disc

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Parallel solid-phase isothermal amplification and detection of multiple DNA targets in microliter-sized wells of a digital versatile disc

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dc.contributor.author Santiago Felipe, Sara es_ES
dc.contributor.author Tortajada-Genaro, Luis Antonio es_ES
dc.contributor.author Puchades, Rosa es_ES
dc.contributor.author Maquieira Catala, Ángel es_ES
dc.date.accessioned 2016-09-12T06:47:33Z
dc.date.available 2016-09-12T06:47:33Z
dc.date.issued 2016-03
dc.identifier.issn 0026-3672
dc.identifier.uri http://hdl.handle.net/10251/69238
dc.description.abstract An integrated method for the parallelized detection of multiple DNA target sequences is presented by using microstructures in a digital versatile disc (DVD). Samples and reagents were managed by using both the capillary and centrifugal forces induced by disc rotation. Recombinase polymerase amplification (RPA), in a bridge solid phase format, took place in separate wells, which thereby modified their optical properties. Then the DVD drive reader recorded the modifications of the transmitted laser beam. The strategy allowed tens of genetic determinations to be made simultaneously within < 2 h, with small sample volumes (3 mu L), low manipulation and at low cost. The method was applied to high-throughput screening of relevant safety threats (allergens, GMOs and pathogenic bacteria) in food samples. Satisfactory results were obtained in terms of sensitivity (48.7 fg of DNA) and reproducibility (below 18 %). This scheme warrants cost-effective multiplex amplification and detection and is perceived to represent a viable tool for screening of nucleic acid targets. es_ES
dc.description.sponsorship This research has been funded through projects FEDER PrometeoII/2014/040 (GVA), and CTQ/2013/45875-R (MINECO). The Spanish Ministry of Education and Science provided S.S.F. with a grant for PhD studies. en_EN
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation projects FEDER PrometeoII/2014/040 CTQ/2013/45875-R es_ES
dc.relation Spanish Ministry of Education and Science es_ES
dc.relation.ispartof Microchimica Acta es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Multiplex PCR es_ES
dc.subject DNA es_ES
dc.subject Recombinase polymerase amplification (RPA) es_ES
dc.subject Compact disc es_ES
dc.subject High-throughput screening es_ES
dc.subject Food safety es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Parallel solid-phase isothermal amplification and detection of multiple DNA targets in microliter-sized wells of a digital versatile disc es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s00604-016-1745-3
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.description.bibliographicCitation Santiago Felipe, S.; Tortajada-Genaro, LA.; Puchades, R.; Maquieira Catala, Á. (2016). Parallel solid-phase isothermal amplification and detection of multiple DNA targets in microliter-sized wells of a digital versatile disc. Microchimica Acta. 183(3):1195-1202. doi:10.1007/s00604-016-1745-3 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1007/s00604-016-1745-3 es_ES
dc.description.upvformatpinicio 1195 es_ES
dc.description.upvformatpfin 1202 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 183 es_ES
dc.description.issue 3 es_ES
dc.relation.senia 306763 es_ES
dc.identifier.eissn 1436-5073
dc.relation.references Li Y, Guo SJ, Shao N, Tu S, Xu M, Ren ZR, Ling X, Wang GQ, Lin ZX, Tao SC (2011) A universal multiplex PCR strategy for 100-plex amplification using a hydrophobically patterned microarray. Lab Chip 11:3609–3618 es_ES
dc.relation.references Ng JK, Chong S (2011) Multiplexing Capabilities of Biosensors for Clinical Diagnostics. INTECH Open Access Publisher, Rijeka es_ES
dc.relation.references Shrestha HK, Hwu KK, Chang MC (2010) Advances in detection of genetically engineered crops by multiplex polymerase chain reaction methods. Trends Food Sci Tech 21:442–454 es_ES
dc.relation.references Shao N, Jiang SM, Zhang M, Wang J, Guo SJ, Li Y, Jiang HW, Liu CX, Zhang DB, Yang LT, Tao SC (2014) MACRO: a combined microchip-PCR and microarray system for high-throughput monitoring of genetically modified organisms. Anal Chem 86:1269–1276 es_ES
dc.relation.references Baker M (2012) Digital PCR hits its stride. Nat Methods 9:541–544 es_ES
dc.relation.references Hoffmann J, Trotter M, von Stetten F, Zengerle R, Roth G (2012) Solid-phase PCR in a picowell array for immobilizing and arraying 100,000 PCR products to a microscope slide. Lab Chip 12:3049–3054 es_ES
dc.relation.references Gole J, Gore A, Richards A, Chiu YJ, Fung HL, Bushman D, Chiang HI, Chun J, Lo YH, Zhang K (2013) Massively parallel polymerase cloning and genome sequencing of single cells using nanoliter microwells. Nat Biotechnol 31:1126–1132 es_ES
dc.relation.references McCalla SE, Tripathi A (2011) Microfluidic reactors for diagnostics applications. Annu Rev. Biomed Eng 13:321–343 es_ES
dc.relation.references Westin L, Xu X, Miller C, Wang L, Edman CF, Nerenberg M (2000) Anchored multiplex amplification on a microelectronic chip array. Nat Biotechnol 18:199–204 es_ES
dc.relation.references Khan Z, Poetter K, Park DJ (2008) Enhanced solid phase PCR: mechanisms to increase priming by solid support primers. Anal Biochem 375:391–393 es_ES
dc.relation.references Khodakov DA, Ellis AV (2014) Recent developments in nucleic acid identification using solid-phase enzymatic assays. Microchim Acta 181:1633–1646 es_ES
dc.relation.references Shin Y, Perera AP, Kim KW, Park MK (2013) Real-time, label-free isothermal solid-phase amplification/detection (ISAD) device for rapid detection of genetic alteration in cancers. Lab Chip 13:2106–2114 es_ES
dc.relation.references Kersting S, Rausch V, Bier FF, von Nickisch-Rosenegk M (2014) Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis. Malaria J 13:99–107 es_ES
dc.relation.references del Río JS, Yehia Adly N, Acero-Sánchez JL, Henry OY, O’Sullivan CK (2014) Electrochemical detection of Francisella tularensis genomic DNA using solid-phase recombinase polymerase amplification. Biosens Bioelectron 54: 674–678 es_ES
dc.relation.references Lutz S, Weber P, Focke M, Faltin B, Hoffmann J, Müller C, Mark D, Roth G, Munday P, Armes N, Piepenburg O, Zengerle R, von Stetten F (2010) Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA). Lab Chip 10:887–893 es_ES
dc.relation.references Santiago-Felipe S, Tortajada-Genaro LA, Morais S, Puchades R, Maquieira A (2014) One-pot isothermal DNA amplification–Hybridisation and detection by a disc-based method. Sensor Actuat B-Chem 204:273–281 es_ES
dc.relation.references Tortajada-Genaro LA, Santiago-Felipe S, Amasia M, Russom A, Maquieira A (2015) Isothermal solid-phase recombinase polymerase amplification on microfluidic digital versatile discs (DVDs). RSC Adv 5:29,987–29,995 es_ES
dc.relation.references Wu J, Zhu Y, Xue F, Mei Z, Yao L, Wang X, Zheng L, Liu J, Liu G, Peng C, Chen W (2014) Recent trends in SELEX technique and its application to food safety monitoring. Microchimica Acta 181:479–491 es_ES
dc.relation.references Roda A, Mirasoli M, Roda B, Bonvicini F, Colliva C, Reschiglian P (2012) Recent developments in rapid multiplexed bioanalytical methods for foodborne pathogenic bacteria detection. Microchimica Acta 178:7–28 es_ES
dc.relation.references Xu S (2012) Electromechanical biosensors for pathogen detection. Microchimica Acta 178:245–260 es_ES
dc.relation.references Santiago-Felipe S, Tortajada-Genaro LA, Puchades R, Maquieira A (2014) Recombinase polymerase and enzyme-linked immunosorbent assay as a DNA amplification-detection strategy for food analysis. Anal Chim Acta 811:81–87 es_ES
dc.relation.references International Organization for Standardization, Geneva, Switzerland (2005a) ISO 21569:2005. Foodstuffs-methods of analysis for the detection of genetically modified organisms and derived products-Qualitative nucleic acid based methods es_ES
dc.relation.references International Organization for Standardization, Geneva, Switzerland (2005b) ISO 21570:2005. Foodstuffs-methods of analysis for the detection of genetically modified organisms and derived products-Quantitative nucleic acid based methods es_ES
dc.relation.references Tortajada-Genaro LA, Rodrigo A, Hevia E, Mena S, Niñoles R, Maquieira A (2015) Microarray on digital versatile disc for identification and genotyping of Salmonella and Campylobacter in meat products. Analytical and bioanalytical chemistry 407:7285–7294 es_ES
dc.relation.references Adessi C, Matton G, Ayala G, Turcatti G, Mermod JJ, Mayer P, Kawashima E (2006) Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms. Nucleic Acids Res 28:e87 es_ES
dc.relation.references Shin Y, Kim J, Lee TY (2014) A solid phase-bridge based DNA amplification technique with fluorescence signal enhancement for detection of cancer biomarkers. Sensor Actuat B-Chem 199:220–225 es_ES
dc.relation.references Li G, Chen Q, Li J, Hu X, Zhao J (2010) A compact disk-like centrifugal microfluidic system for high-throughput nanoliter-scale protein crystallization screening. Anal Chem 82:4362–4369 es_ES
dc.relation.references Zhou QJ, Wang L, Chen J, Wang RN, Shi YH, Li CH, Zhang DM, Yan XJ, Zhang YJ (2014) Development and evaluation of a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip LAMP) for rapid and simultaneous detection of ten pathogenic bacteria in aquatic animals. J Microbiol Meth 104:26–35 es_ES
dc.relation.references Focke M, Stumpf F, Roth G, Zengerle R, von Stetten F (2010) Centrifugal microfluidic system for primary amplification and secondary real-time PCR. Lab Chip 10:3210–3212 es_ES
dc.relation.references Gorkin R, Park J, Siegrist J, Amasia M, Lee BS, Park JM, Kim J, Kim H, Madou M, Cho YK (2010) Centrifugal microfluidics for biomedical applications. Lab Chip 10:1758–1773 es_ES


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