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Complex dynamics of defective interfering baculoviruses during serial passage in insect cells

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Complex dynamics of defective interfering baculoviruses during serial passage in insect cells

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dc.contributor.author Zwart, Mark Peter es_ES
dc.contributor.author Pijlman, G.P. es_ES
dc.contributor.author Sardanyes Cayuela, Jose es_ES
dc.contributor.author Duarte, J es_ES
dc.contributor.author Januario, C es_ES
dc.contributor.author Elena Fito, Santiago Fco es_ES
dc.date.accessioned 2016-09-19T10:58:42Z
dc.date.available 2016-09-19T10:58:42Z
dc.date.issued 2013-03
dc.identifier.issn 0092-0606
dc.identifier.uri http://hdl.handle.net/10251/70076
dc.description.abstract Defective interfering (DI) viruses are thought to cause oscillations in virus levels, known as the 'Von Magnus effect'. Interference by DI viruses has been proposed to underlie these dynamics, although experimental tests of this idea have not been forthcoming. For the baculoviruses, insect viruses commonly used for the expression of heterologous proteins in insect cells, the molecular mechanisms underlying DI generation have been investigated. However, the dynamics of baculovirus populations harboring DIs have not been studied in detail. In order to address this issue, we used quantitative real-time PCR to determine the levels of helper and DI viruses during 50 serial passages of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) in Sf21 cells. Unexpectedly, the helper and DI viruses changed levels largely in phase, and oscillations were highly irregular, suggesting the presence of chaos. We therefore developed a simple mathematical model of baculovirus-DI dynamics. This theoretical model reproduced patterns qualitatively similar to the experimental data. Although we cannot exclude that experimental variation (noise) plays an important role in generating the observed patterns, the presence of chaos in the model dynamics was confirmed with the computation of the maximal Lyapunov exponent, and a Ruelle-Takens-Newhouse route to chaos was identified at decreasing production of DI viruses, using mutation as a control parameter. Our results contribute to a better understanding of the dynamics of DI baculoviruses, and suggest that changes in virus levels over passages may exhibit chaos. es_ES
dc.description.sponsorship The authors thank Javier Carrera, Just Vlak and Lia Hemerik for helpful discussion. MPZ was supported by a Rubicon Grant from the Netherlands Organization for Scientific Research (NWO, www.nwo.nl) and a 'Juan de la Cierva' postdoctoral contract (JCI-2011-10379) from the Spanish 'Secretaria de Estado de Investigacion, Desarrollo e Innovacion'. JS was supported by the Botin Foundation. SFE was supported by grant BFU2012-30805, also from the Spanish 'Secretaria de Estado de Investigacion, Desarrollo e Innovacion'. en_EN
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation Netherlands Organization for Scientific Research (NWO) es_ES
dc.relation Spanish 'Secretaria de Estado de Investigacion, Desarrollo e Innovacion' JCI-2011-10379 BFU2012-30805 es_ES
dc.relation Botin Foundation es_ES
dc.relation.ispartof Journal of Biological Physics es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Baculovirus es_ES
dc.subject Bifurcations es_ES
dc.subject Chaos es_ES
dc.subject Defective interfering virus es_ES
dc.subject Experimental evolution es_ES
dc.title Complex dynamics of defective interfering baculoviruses during serial passage in insect cells es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s10867-013-9317-9
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Zwart, MP.; Pijlman, G.; Sardanyes Cayuela, J.; Duarte, J.; Januario, C.; Elena Fito, SF. (2013). Complex dynamics of defective interfering baculoviruses during serial passage in insect cells. Journal of Biological Physics. 39(2):327-342. doi:10.1007/s10867-013-9317-9 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1007/s10867-013-9317-9 es_ES
dc.description.upvformatpinicio 327 es_ES
dc.description.upvformatpfin 342 es_ES
dc.type.version info:eu repo/semantics/publishedVersion es_ES
dc.description.volume 39 es_ES
dc.description.issue 2 es_ES
dc.relation.senia 260378 es_ES
dc.identifier.pmcid PMC3662412 en_EN


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