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Complex dynamics of defective interfering baculoviruses during serial passage in insect cells

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Complex dynamics of defective interfering baculoviruses during serial passage in insect cells

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dc.contributor.author Zwart, Mark Peter es_ES
dc.contributor.author Pijlman, G.P. es_ES
dc.contributor.author Sardanyes Cayuela, Jose es_ES
dc.contributor.author Duarte, J es_ES
dc.contributor.author Januario, C es_ES
dc.contributor.author Elena Fito, Santiago Fco es_ES
dc.date.accessioned 2016-09-19T10:58:42Z
dc.date.available 2016-09-19T10:58:42Z
dc.date.issued 2013-03
dc.identifier.issn 0092-0606
dc.identifier.uri http://hdl.handle.net/10251/70076
dc.description.abstract Defective interfering (DI) viruses are thought to cause oscillations in virus levels, known as the 'Von Magnus effect'. Interference by DI viruses has been proposed to underlie these dynamics, although experimental tests of this idea have not been forthcoming. For the baculoviruses, insect viruses commonly used for the expression of heterologous proteins in insect cells, the molecular mechanisms underlying DI generation have been investigated. However, the dynamics of baculovirus populations harboring DIs have not been studied in detail. In order to address this issue, we used quantitative real-time PCR to determine the levels of helper and DI viruses during 50 serial passages of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) in Sf21 cells. Unexpectedly, the helper and DI viruses changed levels largely in phase, and oscillations were highly irregular, suggesting the presence of chaos. We therefore developed a simple mathematical model of baculovirus-DI dynamics. This theoretical model reproduced patterns qualitatively similar to the experimental data. Although we cannot exclude that experimental variation (noise) plays an important role in generating the observed patterns, the presence of chaos in the model dynamics was confirmed with the computation of the maximal Lyapunov exponent, and a Ruelle-Takens-Newhouse route to chaos was identified at decreasing production of DI viruses, using mutation as a control parameter. Our results contribute to a better understanding of the dynamics of DI baculoviruses, and suggest that changes in virus levels over passages may exhibit chaos. es_ES
dc.description.sponsorship The authors thank Javier Carrera, Just Vlak and Lia Hemerik for helpful discussion. MPZ was supported by a Rubicon Grant from the Netherlands Organization for Scientific Research (NWO, www.nwo.nl) and a 'Juan de la Cierva' postdoctoral contract (JCI-2011-10379) from the Spanish 'Secretaria de Estado de Investigacion, Desarrollo e Innovacion'. JS was supported by the Botin Foundation. SFE was supported by grant BFU2012-30805, also from the Spanish 'Secretaria de Estado de Investigacion, Desarrollo e Innovacion'. en_EN
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation.ispartof Journal of Biological Physics es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Baculovirus es_ES
dc.subject Bifurcations es_ES
dc.subject Chaos es_ES
dc.subject Defective interfering virus es_ES
dc.subject Experimental evolution es_ES
dc.title Complex dynamics of defective interfering baculoviruses during serial passage in insect cells es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s10867-013-9317-9
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BFU2012-30805/ES/EVOLUTIONARY SYSTEMS VIROLOGY: EPISTASIS AND THE RUGGEDNESS OF ADAPTIVE LANDSCAPES, MUTATIONS IN REGULATORY SEQUENCES, AND THE HOST DETERMINANTS OF VIRAL FITNESS/ / es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//JCI-2011-10379/ES/JCI-2011-10379/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Zwart, MP.; Pijlman, G.; Sardanyes Cayuela, J.; Duarte, J.; Januario, C.; Elena Fito, SF. (2013). Complex dynamics of defective interfering baculoviruses during serial passage in insect cells. Journal of Biological Physics. 39(2):327-342. doi:10.1007/s10867-013-9317-9 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1007/s10867-013-9317-9 es_ES
dc.description.upvformatpinicio 327 es_ES
dc.description.upvformatpfin 342 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 39 es_ES
dc.description.issue 2 es_ES
dc.relation.senia 260378 es_ES
dc.identifier.pmid 23860876 en_EN
dc.identifier.pmcid PMC3662412 en_EN
dc.contributor.funder Fundación Botín es_ES
dc.contributor.funder Netherlands Organization for Scientific Research es_ES
dc.description.references Von Magnus, P.: Incomplete forms of influenza virus. Adv. Virus. Res. 2, 59–79 (1954) es_ES
dc.description.references Huang, A.S.: Defective interfering viruses. Annu. Rev. Microbiol. 27, 101–117 (1973) es_ES
dc.description.references Kool, M., Voncken, J.W., Vanlier, F.L.J., Tramper, J., Vlak, J.M.: Detection and analysis of Autographa californica nuclear polyhedrosis-virus mutants with defective interfering properties. Virology 183, 739–746 (1991) es_ES
dc.description.references Wickham, T.J., Davis, T., Granados, R.R., Hammer, D.A., Shuler, M.L., Wood, H.A.: Baculovirus defective interfering particles are responsible for variations in recombinant protein-production as a function of multiplicity of infection. Biotechnol. Lett. 13, 483–488 (1991) es_ES
dc.description.references Pijlman, G.P., van den Born, E., Martens, D.E., Vlak, J.M.: Autographa californica baculoviruses with large genomic deletions are rapidly generated in infected insect cells. Virology 283, 132–138 (2001) es_ES
dc.description.references Giri, L., Feiss, M.G., Bonning, B.C., Murhammer, D.W.: Production of baculovirus defective interfering particles during serial passage is delayed by removing transposon target sites in fp25k. J. Gen. Virol. 93, 389–399 (2012) es_ES
dc.description.references King, L.A., Possee, R.D.: The Baculovirus Expression System. University Press, Cambridge (1992) es_ES
dc.description.references Lee, H.Y., Krell, P.J.: Reiterated DNA fragments in defective genomes of Autographa californica nuclear polyhedrosis virus are competent for AcMNPV-dependent DNA replication. Virology 202, 418–429 (1994) es_ES
dc.description.references Pijlman, G.P., Dortmans, J., Vermeesch, A.M.G., Yang, K., Martens, D.E., Goldbach, R.W., Vlak, J.M.: Pivotal role of the non-hr origin of DNA replication in the genesis of defective interfering baculoviruses. J. Virol. 76, 5605–5611 (2002) es_ES
dc.description.references Pijlman, G.P., van Schijndel, J.E., Vlak, J.M.: Spontaneous excision of BAC vector sequences from bacmid-derived baculovirus expression vectors upon passage in insect cells. J. Gen. Virol. 84, 2669–2678 (2003) es_ES
dc.description.references Pijlman, G.P., Vermeesch, A.M.G., Vlak, J.M.: Cell line-specific accumulation of the baculovirus non-hr origin of DNA replication in infected insect cells. J. Invertebr. Pathol. 84, 214–219 (2003) es_ES
dc.description.references Roux, L., Simon, A.E., Holland, J.J.: Effects of defective interfering viruses on virus-replication and pathogenesis in vitro and in vivo. Adv. Virus. Res. 40, 181–211 (1991) es_ES
dc.description.references Grabau, E.A., Holland, J.J.: Analysis of viral and defective-interfering nucleocapsids in acute and persistent infection by Rhadoviruses. J. Gen. Virol. 60, 87–97 (1982) es_ES
dc.description.references Kawai, A., Matsumoto, S., Tanabe, K.: Characterization of Rabies viruses recovered from persistently infected BHK cells. Virology 67, 520–533 (1975) es_ES
dc.description.references Roux, L., Holland, J.J.: Viral genome synthesis in BHK-21 cells persistently infected with Sendai virus. Virology 100, 53–64 (1980) es_ES
dc.description.references Palma, E.L., Huang, A.: Cyclic production of vesicular stomatitis virus cause by defective interfering particles. J. Infect. Dis. 129, 402–410 (1974). es_ES
dc.description.references Stauffer Thompson, K.A., Yin, J.: Population dynamics of an RNA virus and its defective interfering particles in passage cultures. Virol. J. 7, 257–266 (2010) es_ES
dc.description.references Szathmáry, E.: Cooperation and defection – playing the field in virus dynamics. J. Theor. Biol. 165, 341–356 (1993) es_ES
dc.description.references Bangham, C.R.M., Kirkwood, T.B.L.: Defective interfering particles – effects in modulating virus growth and persistence. Virology 179, 821–826 (1990) es_ES
dc.description.references Kirkwood, T.B.L., Bangham, C.R.M.: Cycles, chaos, and evolution in virus cultures – a model of defective interfering particles. Proc. Natl. Acad. Sci. USA 91, 8685–8689 (1994) es_ES
dc.description.references De Gooijer, C.D., Koken, R.H.M., van Lier, F.L.J., Kool, M., Vlak, J.M., Tramper, J.: A structured dynamic model for the baculovirus infection process in insect-cell reactor configurations. Biotech. Bioeng. 40, 537–548 (1992) es_ES
dc.description.references Van Lier, F.L.J., van der Meijs, W.C.J., Grobben, N.G., Olie, R.A., Vlak, J.M., Tramper, J.: Continuous beta-galactosidase production with a recombinant baculovirus insect-cell system in bioreactors. J. Biotechnol. 22, 291–298 (1992) es_ES
dc.description.references Van Lier, F.L.J., van den Hombergh, J., de Gooijer, C.D., den Boer, M.M., Vlak, J.M., Tramper, J.: Long-term semi-continuous production of recombinant baculovirus protein in a repeated (fed-)batch two-stage reactor system. Enzyme Microb. Technol. 18, 460–466 (1996) es_ES
dc.description.references Zwart, M.P., Erro, E., van Oers, M.M., de Visser, J.A.G.M., Vlak, J.M.: Low multiplicity of infection in vivo results in purifying selection against baculovirus deletion mutants. J. Gen. Virol. 89, 1220–1224 (2008) es_ES
dc.description.references Luckow, V.A., Lee, S.C., Barry, G.F., Olins, P.O.: Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli. J. Virol. 67, 4566–4579 (1993) es_ES
dc.description.references Vaughn, J.L., Goodwin, R.H., Tompkins, G.J., McCawley, P.: Establishment of 2 cell lines from insect Spodoptera frugiperda (Lepidoptera, Noctuidae). In Vitro 13, 213–217 (1977) es_ES
dc.description.references Zwart, M.P., van Oers, M.M., Cory, J.S., van Lent, J.W.M., van der Werf, W., Vlak, J.M.: Development of a quantitative real-time PCR for determination of genotype frequencies for studies in baculovirus population biology. J. Virol. Meth. 148, 146–154 (2008) es_ES
dc.description.references Zwart, M.P., Hemerik, L., Cory, J.S., de Visser, J.A.G.M., Bianchi, F.J.J.A., van Oers, M.M., Vlak, J.M., Hoekstra, R.F., van der Werf, W.: An experimental test of the independent action hypothesis in virus-insect pathosystems. Proc. R. Soc. B 276, 2233–2242 (2009) es_ES
dc.description.references Olkin, I., Gleser, L.J., Derman, C.: Probability Models and Applications. Macmillan, New York (1994) es_ES
dc.description.references Parker, T., Chua, L.: Practical Numerical Algorithms for Chaotic Systems. Springer-Verlag, Berlin (1989) es_ES
dc.description.references Dieci, L., van Vleck, E.S.: Computation of a few Lyapunov exponents for continuous and discrete dynamical systems. J. Appl. Numer. Math. 17, 275–291 (1995) es_ES
dc.description.references Matsumoto, T., Chua, L.O., Komuro, M.: The double scroll. IEEE Trans. Circuits Syst. 32, 797–818 (1985) es_ES
dc.description.references Chua, L.O., Komuro, M., Matsumoto, T.: The double scroll family: rigorous proof of chaos. IEEE Trans. Circuits Syst. 33, 1072–1097 (1986) es_ES
dc.description.references Ramasubramanian, K., Sriram, M.S.: A comparative study of computation of Lyapunov spectra with different algorithms. Phys. D: Nonlin. Phenom. 139, 72–86 (2000) es_ES
dc.description.references Lee, H.Y., Krell, P.J.: Generation and analysis of defective genomes of Autographa californica nuclear polyhedrosis virus. J. Virol. 66, 4339–4347 (1992) es_ES
dc.description.references Kovacs, G.R., Choi, J., Guarino, L.A., Summers MD: Functional dissection of the Autographa californica nuclear polyhedrosis virus immediate early 1 transcriptional regulatory protein. J. Virol. 66, 7429–7437 (1992) es_ES
dc.description.references Legendre, P., Legendre, L.: Numerical Ecology. Elsevier, Amsterdam (1998) es_ES
dc.description.references Schuster, H.G.: Deterministic Chaos: An Introduction. Wiley-VCH Verlag GmbH & Co. KGaA, Wienheim (2005) es_ES
dc.description.references Strogatz, S.H.: Nonlinear Dynamics and Chaos: With Applications to Physics, Biology, Chemistry and Engineering. Westview Press, Cambridge (1994) es_ES
dc.description.references Dennis, B., Desharnais, R.A., Cushing, J.M., Henson, S.M., Constantino, R.F.: Can noise induce chaos? Oikos 102, 329–339 (2003) es_ES
dc.description.references Crutchfield, J.P., Huberman, B.A.: Fluctuations and the onset of chaos. Phys. Lett. A 77, 407–410 (1980) es_ES
dc.description.references Crutchfield, J.P., Farmer, J.D.: Fluctuations and simple chaotic dynamics. Phys. Rep. 92, 45–82 (1982) es_ES


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