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Simultaneous detection of Clavibacter michiganensis subsp michiganensis, Pepino mosaic virus and Mexican papita viroid by non-radioactive molecular hybridization using a unique polyprobe

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Simultaneous detection of Clavibacter michiganensis subsp michiganensis, Pepino mosaic virus and Mexican papita viroid by non-radioactive molecular hybridization using a unique polyprobe

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dc.contributor.author Zamora-Macorra, E es_ES
dc.contributor.author Ochoa-Martinez, DL es_ES
dc.contributor.author Valdovinos-Ponce, G es_ES
dc.contributor.author Rojas-Martinez, R es_ES
dc.contributor.author Ramirez-Rojas, S es_ES
dc.contributor.author Sanchez Navarro, Jesus Angel es_ES
dc.contributor.author Pallás Benet, Vicente es_ES
dc.contributor.author Aparicio Herrero, Frederic es_ES
dc.date.accessioned 2016-09-28T08:21:26Z
dc.date.available 2016-09-28T08:21:26Z
dc.date.issued 2015 es_ES
dc.identifier.issn 0929-1873
dc.identifier.uri http://hdl.handle.net/10251/70552
dc.description.abstract Clavibacter michiganensis subsp. michiganensis, Pepino mosaic virus, and Mexican papita viroid are three economically important pathogens that infect tomato crops. In this work, a polyprobe (poly-3) was developed and evaluated for the simultaneous detection of these pathogens in tomato plants by non-isotopic molecular hybridization. The endpoint detection limit of the poly-3 with C. michiganensis subsp. michiganensis cell cultures was 10(6) CFU/ml. No differences were found in terms of the sensitivity and specificity when the individual riboprobes were compared to the poly-3 for the detection of the three pathogens. The analysis of 80 tomato field samples by the poly-3 and RT-PCR techniques rendered the same number of positive samples for each pathogen. As far as we know this is the first time that three pathogens with very different life cycle styles (bacteria, virus and viroid) are simultaneously detected in a single assay. The possibility of using this poly-3 technology for the routine diagnosis of field tomato samples is discussed. es_ES
dc.description.sponsorship E.J M-Z. was recipient of a Pre-doctoral fellowship from the Consejo Nacional de Ciencia y Tecnologia of Mexico. F.A. was recipient of a contract Ramon y Cajal (RYC-2010-06169) Program of the Ministerio de Educacion y Ciencia of Spain. We thank L. Corachan for her excellent technical assistance. This work was supported by Grants BIO2014-54862-R from the Spanish Granting Agency DGICYT, the Prometeo Program GV2011/003 from the Generalitat Valenciana and PAID-06-10-1496 from the Universitat Politecnica de Valencia (Spain). en_EN
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation.ispartof European Journal of Plant Pathology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Tomato es_ES
dc.subject Clavibacter michiganensis subsp michiganensis
dc.subject Pepino mosaic
dc.subject Virus
dc.subject Mexican papita viroid
dc.subject Polyriboprobe
dc.subject Non-isotopic molecular hybridization
dc.title Simultaneous detection of Clavibacter michiganensis subsp michiganensis, Pepino mosaic virus and Mexican papita viroid by non-radioactive molecular hybridization using a unique polyprobe es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s10658-015-0729-1
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BIO2014-54862-R/ES/INTERACCIONES ENTRE FACTORES VIRALES Y DEL HUESPED IMPLICADOS EN LOS PROCESOS DE MOVIMIENTO Y PATOGENESIS EN CULTIVOS DE INTERES AGRONOMICO/ / es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//RYC-2010-06169/ES/RYC-2010-06169/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//GV%2F2011%2F003/ES/GV%2F2011%2F003/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UPV//PAID-06-10-1496/ES/PAID-06-10-1496/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Zamora-Macorra, E.; Ochoa-Martinez, D.; Valdovinos-Ponce, G.; Rojas-Martinez, R.; Ramirez-Rojas, S.; Sanchez Navarro, JA.; Pallás Benet, V.... (2015). Simultaneous detection of Clavibacter michiganensis subsp michiganensis, Pepino mosaic virus and Mexican papita viroid by non-radioactive molecular hybridization using a unique polyprobe. European Journal of Plant Pathology. 143(4):779-787. doi:10.1007/s10658-015-0729-1 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1007/s10658-015-0729-1
dc.description.upvformatpinicio 779 es_ES
dc.description.upvformatpfin 787 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 143 es_ES
dc.description.issue 4 es_ES
dc.relation.senia 309362 es_ES
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