Yu, E.; Galiana, I.; Martínez-Máñez, R.; Stroeve, P.; Marcos Martínez, MD.; Aznar, E.; Sancenón Galarza, F.... (2015). Poly(N-isopropylacrylamide)-gated Fe3O4/SiO2 core shell nanoparticles with expanded mesoporous structures for the temperature triggered release of lysozyme. Colloids and Surfaces B: Biointerfaces. 135:652-660. https://doi.org/10.1016/j.colsurfb.2015.06.048
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/71417
Título:
|
Poly(N-isopropylacrylamide)-gated Fe3O4/SiO2 core shell nanoparticles with expanded mesoporous structures for the temperature triggered release of lysozyme
|
Autor:
|
Yu, Erick
Galiana, Irene
Martínez-Máñez, Ramón
Stroeve, Pieter
Marcos Martínez, María Dolores
Aznar, Elena
Sancenón Galarza, Félix
Murguía, Jose R.
Amoros del Toro, Pedro Jose
|
Entidad UPV:
|
Universitat Politècnica de València. Departamento de Química - Departament de Química
Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic
Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia
|
Fecha difusión:
|
|
Resumen:
|
Core-shell nanoparticles comprised of Fe3O4 cores and a mesoporous silica shell with an average expanded pore size of 6.07 nm and coated with a poly(N-isopropylacrylamide) (PNIPAM) layer (CS MSNs EP PNIPAM) were prepared ...[+]
Core-shell nanoparticles comprised of Fe3O4 cores and a mesoporous silica shell with an average expanded pore size of 6.07 nm and coated with a poly(N-isopropylacrylamide) (PNIPAM) layer (CS MSNs EP PNIPAM) were prepared and characterized. The nanoparticles was loaded with (Ru(bipy)3 2+) dye or an antibacterial enzyme, lysozyme, to obtain CS MSNs EP PNIPAM Ru(bipy)3 2+ and CS MSNs EP PNIPAM Lys, respectively. The lysozyme loading was determined to be 160 mg/g of nanoparticle. It was seen that Ru(bipy)3 2+ and lysozyme release was minimal at a room temperature of
25 ºC while at physiological temperature (37 º C), abrupt release was observed. The applicability of the CS MSNs EP PNIPAM Lys was further tested with two Gram-positive bacteria samples, Bacillus cereus and Micrococcus luteus. At physiological temperature, the nanoparticles were shown to reduce bacterial growth, indicating a successful release of lysozyme from the nanoparticles. This nanoparticle system shows potential as a nanocarrier for the loading of similarly sized proteins or other species as a drug delivery platform.
[-]
|
Palabras clave:
|
Protein delivery
,
Triggered release
,
Mesoporous silica
,
Pore expansion
,
PNIPAM
|
Derechos de uso:
|
Cerrado |
Fuente:
|
Colloids and Surfaces B: Biointerfaces. (issn:
0927-7765
) (eissn:
1873-4367
)
|
DOI:
|
10.1016/j.colsurfb.2015.06.048
|
Editorial:
|
Elsevier
|
Versión del editor:
|
http://dx.doi.org/10.1016/j.colsurfb.2015.06.048
|
Código del Proyecto:
|
info:eu-repo/grantAgreement/MINECO//MAT2012-38429-C04-03/ES/NANOMATERIALES HIBRIDOS MESOPOROSOS Y COMPOSITES PARA EL DISEÑO DE SISTEMAS SENSORES, DE SUMINISTRO CONTROLADO Y REMEDIACION/
info:eu-repo/grantAgreement/GVA//PROMETEOII%2F2014%2F047/ES/Nuevas aproximaciones para el diseño de materiales de liberación controlada y la detección de compuestos peligrosos/
info:eu-repo/grantAgreement/MINECO//MAT2012-38429-C04-01/ES/DESARROLLO DE MATERIALES FUNCIONALIZADOS CON PUERTAS NANOSCOPICAS PARA APLICACIONES DE LIBERACION CONTROLADA Y SENSORES PARA LA DETECCION DE NITRATO AMONICO, SULFIDRICO Y CO /
|
Agradecimientos:
|
The authors wish to express their gratitude to the facilities at Ciudad Politecnica de la Innovacion (CPI), El Centro de Reconocimiento Molecular y Desarrollo Tecnologico (IDM), the Biotechnology Department at UPV, and the ...[+]
The authors wish to express their gratitude to the facilities at Ciudad Politecnica de la Innovacion (CPI), El Centro de Reconocimiento Molecular y Desarrollo Tecnologico (IDM), the Biotechnology Department at UPV, and the Instituto de Biologia Molecular y Celular de Plantas (IBMCP) at UPV for assistance in the materials characterization techniques and for providing the bacterial samples for antibacterial testing. Warm thanks are given to the Transatlantic Partnership for Excellence in Engineering (TEE) program, an Erasmus Mundus-Action 2 project by the European Commission, for the opportunity to undertake research at the collaborating university, UPV. The University of California Office of the President (UCOP) Lab Fees Research Program funded this research. The authors also thank the Spanish Government (Projects MAT2012-38429-C04-01 and MAT2012-38429-C04-03), the Generalitat Valenciana (Project PROMETEOII/2014/047) for support.
[-]
|
Tipo:
|
Artículo
|