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dc.contributor.author | Noar, Jesse | es_ES |
dc.contributor.author | Loveless, Telisa | es_ES |
dc.contributor.author | Navarro Herrero, José Luís | es_ES |
dc.contributor.author | Olson, Jonathan W. | es_ES |
dc.contributor.author | Bruno-Barcena, Jose M. | es_ES |
dc.date.accessioned | 2016-10-17T09:04:05Z | |
dc.date.available | 2016-10-17T09:04:05Z | |
dc.date.issued | 2015-07 | |
dc.identifier.issn | 0099-2240 | |
dc.identifier.uri | http://hdl.handle.net/10251/71896 | |
dc.description.abstract | The diazotroph Azotobacter vinelandii possesses three distinct nitrogenase isoenzymes, all of which produce molecular hydrogen as a by-product. In batch cultures, A. vinelandii strain CA6, a mutant of strain CA, displays multiple phenotypes distinct from its parent: tolerance to tungstate, impaired growth and molybdate transport, and increased hydrogen evolution. Determining and comparing the genomic sequences of strains CA and CA6 revealed a large deletion in CA6's genome, encompassing genes related to molybdate and iron transport and hydrogen reoxidation. A series of iron uptake analyses and chemostat culture experiments confirmed iron transport impairment and showed that the addition of fixed nitrogen (ammonia) resulted in cessation of hydrogen production. Additional chemostat experiments compared the hydrogen-producing parameters of different strains: in iron-sufficient, tungstate-free conditions, strain CA6's yields were identical to those of a strain lacking only a single hydrogenase gene. However, in the presence of tungstate, CA6 produced several times more hydrogen. A. vinelandii may hold promise for developing a novel strategy for production of hydrogen as an energy compound. | es_ES |
dc.description.sponsorship | J.N. was the recipient of a 3-year National Science Foundation Graduate Research Fellowship. This project was supported by the North Carolina State University Department of Microbiology (now Plant and Microbial Biology). The University of North Carolina-Chapel Hill Core facility is supported by grant number NIH P30 DK34987. | en_EN |
dc.language | Inglés | es_ES |
dc.publisher | American Society for Microbiology | es_ES |
dc.relation.ispartof | Applied and Environmental Microbiology | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | AMMONIUM-ASSIMILATING CULTURES | es_ES |
dc.subject | 2ND ALTERNATIVE NITROGENASE | es_ES |
dc.subject | RHODOBACTER-CAPSULATUS | es_ES |
dc.subject | THERMOTOGA-NEAPOLITANA | es_ES |
dc.subject | STRUCTURAL GENES | es_ES |
dc.subject | Transcriptional regulation | es_ES |
dc.subject | ENTEROBACTER-AEROGENES | es_ES |
dc.subject | KLEBSIELLA-PNEUMONIAE | es_ES |
dc.subject | MOLYBDENUM TRANSPORT | es_ES |
dc.subject | MUTATIONAL ANALYSIS | es_ES |
dc.subject.classification | INGENIERIA DE SISTEMAS Y AUTOMATICA | es_ES |
dc.title | Aerobic Hydrogen Production via Nitrogenase in Azotobacter vinelandii CA6 | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1128/AEM.00679-15 | |
dc.relation.projectID | info:eu-repo/grantAgreement/NIH//P30DK034987/ | es_ES |
dc.rights.accessRights | Cerrado | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Ingeniería de Sistemas y Automática - Departament d'Enginyeria de Sistemes i Automàtica | es_ES |
dc.description.bibliographicCitation | Noar, J.; Loveless, T.; Navarro Herrero, JL.; Olson, JW.; Bruno-Barcena, JM. (2015). Aerobic Hydrogen Production via Nitrogenase in Azotobacter vinelandii CA6. Applied and Environmental Microbiology. 81(13):4507-4516. doi:10.1128/AEM.00679-15 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1128/AEM.00679-15 | es_ES |
dc.description.upvformatpinicio | 4507 | es_ES |
dc.description.upvformatpfin | 4516 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 81 | es_ES |
dc.description.issue | 13 | es_ES |
dc.relation.senia | 296327 | es_ES |
dc.contributor.funder | National Science Foundation, EEUU | es_ES |
dc.contributor.funder | National Institutes of Health, EEUU | es_ES |
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