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Direct covalent attachment of DNA microarrays by rapid thiol-ene "click" chemistry

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Direct covalent attachment of DNA microarrays by rapid thiol-ene "click" chemistry

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Escorihuela Fuentes, J.; Bañuls Polo, M.; Grijalvo-Torrijo, S.; Eritja, R.; Puchades, R.; Maquieira Catala, Á. (2014). Direct covalent attachment of DNA microarrays by rapid thiol-ene "click" chemistry. Bioconjugate Chemistry. 25(3):618-627. doi:10.1021/bc500033d

Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/74785

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Title: Direct covalent attachment of DNA microarrays by rapid thiol-ene "click" chemistry
Author:
UPV Unit: Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural
Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic
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Abstract:
[EN] A rapid strategy for the covalent immobilization of DNA onto silicon-based materials using the UV-initiated radical thiol-ene reaction is presented in this study. Following this approach, thiol- and alkene-modified ...[+]
Subjects: SELF-ASSEMBLED MONOLAYERS , VERSATILE APPROACH , SURFACE , SILICON , IMMOBILIZATION , BIOSENSORS , FLUORESCENCE , PLATFORMS , PROTEINS , XPS
Copyrigths: Cerrado
Source:
Bioconjugate Chemistry. (issn: 1043-1802 )
DOI: 10.1021/bc500033d
Publisher:
American Chemical Society
Publisher version: https://dx.doi.org/10.1021/bc500033d
Thanks:
Financial support from Ministerio de Ciencia e Innovacion (CTQ2010-15943) and Generalitat Valenciana (PROMETEO/2010/008 and ACOMP/2012/158) is acknowledged. The authors are grateful to the Servei d'Espectrometria de Masses ...[+]
Type: Artículo

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