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Direct covalent attachment of DNA microarrays by rapid thiol-ene "click" chemistry

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Direct covalent attachment of DNA microarrays by rapid thiol-ene "click" chemistry

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dc.contributor.author Escorihuela Fuentes, Jorge es_ES
dc.contributor.author Bañuls Polo, María-José es_ES
dc.contributor.author Grijalvo-Torrijo, Santiago es_ES
dc.contributor.author Eritja, Ramón es_ES
dc.contributor.author Puchades, Rosa es_ES
dc.contributor.author Maquieira Catala, Ángel es_ES
dc.date.accessioned 2016-11-30T08:22:45Z
dc.date.issued 2014-03
dc.identifier.issn 1043-1802
dc.identifier.uri http://hdl.handle.net/10251/74785
dc.description.abstract [EN] A rapid strategy for the covalent immobilization of DNA onto silicon-based materials using the UV-initiated radical thiol-ene reaction is presented in this study. Following this approach, thiol- and alkene-modified oligonucleotide probes were covalently attached in microarray format, reaching immobilization densities around 6 pmol-cm(-2). The developed methodology presents the advantages of spatially controlled probe anchoring (using a photomask), direct attachment without using cross-linkers (one-pot fashion), and short irradiation times (20 min). Using the described strategy, hybridization efficiencies up to 65% with full complementary strands were reached. The approach was evaluated by scoring single-base pair mismatches with discrimination ratios around 15. Moreover, the efficacy of the proposed DNA detection scheme is further demonstrated through the assay on a genomic target of bacterial Escherichia coli. es_ES
dc.description.sponsorship Financial support from Ministerio de Ciencia e Innovacion (CTQ2010-15943) and Generalitat Valenciana (PROMETEO/2010/008 and ACOMP/2012/158) is acknowledged. The authors are grateful to the Servei d'Espectrometria de Masses of the Universitat de Barcelona for the spectroscopic facilities. en_EN
dc.language Inglés es_ES
dc.publisher American Chemical Society es_ES
dc.relation.ispartof Bioconjugate Chemistry es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject SELF-ASSEMBLED MONOLAYERS es_ES
dc.subject VERSATILE APPROACH es_ES
dc.subject SURFACE es_ES
dc.subject SILICON es_ES
dc.subject IMMOBILIZATION es_ES
dc.subject BIOSENSORS es_ES
dc.subject FLUORESCENCE es_ES
dc.subject PLATFORMS es_ES
dc.subject PROTEINS es_ES
dc.subject XPS es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Direct covalent attachment of DNA microarrays by rapid thiol-ene "click" chemistry es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1021/bc500033d
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//CTQ2010-15943/ES/ESTUDIO DE NUEVAS VIAS DE DESARROLLO DE BIOMEMS PARA SCREENING MASIVO. DEMOSTRACION DE CONCEPTO COMO HERRAMIENTA DE ANALISIS APLICABLE EN "OMICAS"./ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//ACOMP%2F2012%2F158/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2010%2F008/ES/AYUDA PARA EL GRUPO SYM-IDM/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic es_ES
dc.description.bibliographicCitation Escorihuela Fuentes, J.; Bañuls Polo, M.; Grijalvo-Torrijo, S.; Eritja, R.; Puchades, R.; Maquieira Catala, Á. (2014). Direct covalent attachment of DNA microarrays by rapid thiol-ene "click" chemistry. Bioconjugate Chemistry. 25(3):618-627. https://doi.org/10.1021/bc500033d es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1021/bc500033d es_ES
dc.description.upvformatpinicio 618 es_ES
dc.description.upvformatpfin 627 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 25 es_ES
dc.description.issue 3 es_ES
dc.relation.senia 267659 es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Generalitat Valenciana es_ES


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