Rogina, A.; Rico Tortosa, PM.; Gallego-Ferrer, G.; Ivankovic, M.; Ivankovic, H. (2015). Effect of in situ formed hydroxyapatite on microstructure of freeze-gelled chitosan-based biocomposite scaffolds. European Polymer Journal. 68:278-287. https://doi.org/10.1016/j.eurpolymj.2015.05.004
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/75422
Title:
|
Effect of in situ formed hydroxyapatite on microstructure of freeze-gelled chitosan-based biocomposite scaffolds
|
Author:
|
Rogina, Anamarija
Rico Tortosa, Patricia María
Gallego-Ferrer, Gloria
Ivankovic, Marica
Ivankovic, Hrvoje
|
UPV Unit:
|
Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular
Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials
|
Issued date:
|
|
Abstract:
|
New in situ highly-porous chitosan/hydroxyapatite (CS/HA) biocomposite scaffolds have been prepared via freeze-gelation technique. Different content of in situ synthesized hydroxyapatite within chitosan solution was obtained ...[+]
New in situ highly-porous chitosan/hydroxyapatite (CS/HA) biocomposite scaffolds have been prepared via freeze-gelation technique. Different content of in situ synthesized hydroxyapatite within chitosan solution was obtained by changing the amount of calcium and phosphate precursors. The composition of precipitated inorganic phase was characterized by X-ray diffraction analysis (XRD) and Fourier transformed infrared spectroscopy (FTIR), while morphology of scaffolds was imaged by scanning electron microscopy (SEM). SEM observations of cross section and surface area of prepared scaffolds have shown different microstructure and topography regarding to the HA content, which plays an important role in cell adhesion and proliferation, and nutrient transport. The MIT assay of scaffolds with different content of hydroxyapatite has shown no toxicity which is one of the main requirements for potential biomedical application. Likewise, the presented synthesis allows preparing the scaffolds with large and very well interconnected pores without obtaining toxic intermediate products.
[-]
|
Subjects:
|
Chitosan (CS)
,
In situ hydoxyapatite (HA)
,
Freeze-gelation
,
Highly-porous structure
,
Cytotoxicity
|
Copyrigths:
|
Cerrado |
Source:
|
European Polymer Journal. (issn:
0014-3057
) (eissn:
1873-1945
)
|
DOI:
|
10.1016/j.eurpolymj.2015.05.004
|
Publisher:
|
Elsevier
|
Publisher version:
|
http://dx.doi.org/10.1016/j.eurpolymj.2015.05.004
|
Project ID:
|
info:eu-repo/grantAgreement/MINECO//MAT2013-46467-C4-1-R/ES/ESTIMULACION MECANICA LOCAL DE CELULAS MESENQUIMALES DE CARA A SU DIFERENCIACION OSTEOGENICA Y CONDROGENICA EN MEDICINA REGENERATIVA/
info:eu-repo/grantAgreement/MZOS//125-1252970-3005/HR/Bioceramic, Polymer and Composite Nanostructured Materials/
|
Thanks:
|
CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program. CIBER Actions are financed by the Instituto de Salud Carlos III with assistance from the European ...[+]
CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program. CIBER Actions are financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund.
[-]
|
Type:
|
Artículo
|