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Characterization of the interaction between hepatitis C virus NS5B and the human oestrogen receptor alpha

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Characterization of the interaction between hepatitis C virus NS5B and the human oestrogen receptor alpha

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dc.contributor.author Hillung, Julia es_ES
dc.contributor.author Ruiz-Lopez, Elena es_ES
dc.contributor.author Bellon-Echeverria, Itxaso es_ES
dc.contributor.author Clemente-Casares, Pilar es_ES
dc.contributor.author Mas, Antonio es_ES
dc.date.accessioned 2017-01-13T09:22:36Z
dc.date.available 2017-01-13T09:22:36Z
dc.date.issued 2012-04
dc.identifier.issn 0022-1317
dc.identifier.uri http://hdl.handle.net/10251/76744
dc.description.abstract [EN] The RNA-dependent RNA polymerase (NS5B) of hepatitis C virus (HCV) is part of the viral replicative complex and plays a crucial role in HCV replication. It has been described that NS5B interacts with cellular proteins, and that interactions between NS5B and host proteins are crucial for viral replication. Some of the host factors involved in the HCV replication cycle include the oestrogen receptor alpha (ESR1), protein kinases (c-Src) and chaperones (Hsp70). In this report, we determine the requirements for the interplay between NS5B and the domain C of ESR1 (ESR1C) by using Forster Resonance Energy Transfer. NS5B-ESR1C and ESR1C-ESR1C interactions are dependent on ionic strength, indicating that contacts are mainly electrostatic. Additionally, NS5B residues involved in NS5B oligomerization were also essential for NS5B-ESR1C interaction. The study of the interactions among viral and host factors will provide data to establish innovative therapeutic strategies and the development of new antiviral drugs es_ES
dc.description.sponsorship Authors gratefully acknowledge Dr Watashi for providing oestrogen receptor domain C and derivative constructs. This work was supported by Consejeria de Educacion de Castilla La Mancha (grant no. PPII10-0243-6857) and Ministerio de Ciencia e Innovacion (BFU2010-18767). I. B. E. was supported by predoctoral fellowships from Fundacion para la Investigacion Sanitaria de Castilla-La Mancha (grant no. MOV-2006_JI/06).
dc.language Inglés es_ES
dc.publisher Microbiology Society es_ES
dc.relation.ispartof Journal of General Virology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.title Characterization of the interaction between hepatitis C virus NS5B and the human oestrogen receptor alpha es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1099/vir.0.039396-0
dc.relation.projectID info:eu-repo/grantAgreement/JCCM//PPII10-0243-6857/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BFU2010-18767/ES/INTERACCIONES PROTEINA PROTEINA COMO MODULADORAS DE LA TRANSICION INICIACION ELONGACION EN EL CICLO DE SINTESIS DE LA ARN POLIMERASA DEL VIRUS DE LA HEPATITIS C/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Junta de Comunidades de Castilla-La Mancha//MOV-2006_JI%2F06/ES/MOV-2006_JI%2F06/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Hillung, J.; Ruiz-Lopez, E.; Bellon-Echeverria, I.; Clemente-Casares, P.; Mas, A. (2012). Characterization of the interaction between hepatitis C virus NS5B and the human oestrogen receptor alpha. Journal of General Virology. 93:780-785. https://doi.org/10.1099/vir.0.039396-0 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1099/vir.0.039396-0 es_ES
dc.description.upvformatpinicio 780 es_ES
dc.description.upvformatpfin 785 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 93 es_ES
dc.relation.senia 232662 es_ES
dc.identifier.pmid 22170636
dc.contributor.funder Junta de Comunidades de Castilla-La Mancha
dc.contributor.funder Ministerio de Ciencia e Innovación
dc.contributor.funder Fundación Sociosanitaria de Castilla la Mancha
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