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Deciphering dynamic dose responses of natural promoters and single cis elements upon osmotic and oxidative stress in yeast

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Deciphering dynamic dose responses of natural promoters and single cis elements upon osmotic and oxidative stress in yeast

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dc.contributor.author Dolz Edo, Laura es_ES
dc.contributor.author Rienzo, Alessandro es_ES
dc.contributor.author Poveda Huertes, Daniel es_ES
dc.contributor.author Pascual-Ahuir Giner, María Desamparados es_ES
dc.contributor.author Proft, Markus Hans es_ES
dc.date.accessioned 2017-03-13T10:48:27Z
dc.date.available 2017-03-13T10:48:27Z
dc.date.issued 2013-06
dc.identifier.issn 0270-7306
dc.identifier.uri http://hdl.handle.net/10251/78701
dc.description.abstract [EN] Fine-tuned activation of gene expression in response to stress is the result of dynamic interactions of transcription factors with specific promoter binding sites. In the study described here we used a time-resolved luciferase reporter assay in living Saccharomyces cerevisiae yeast cells to gain insights into how osmotic and oxidative stress signals modulate gene expression in a dose-sensitive manner. Specifically, the dose-response behavior of four different natural promoters (GRE2, CTT1, SOD2, and CCP1) reveals differences in their sensitivity and dynamics in response to different salt and oxidative stimuli. Characteristic dose-response profiles were also obtained for artificial promoters driven by only one type of stress-regulated consensus element, such as the cyclic AMP-responsive element, stress response element, or AP-1 site. Oxidative and osmotic stress signals activate these elements separately and with different sensitivities through different signaling molecules. Combination of stress-activated cis elements does not, in general, enhance the absolute expression levels; however, specific combinations can increase the inducibility of the promoter in response to different stress doses. Finally, we show that the stress tolerance of the cell critically modulates the dynamics of its transcriptional response in the case of oxidative stress. es_ES
dc.description.sponsorship This work was supported by the Ministerio de Economa y Competitividad (grant BFU2011-23326 to M.P.) and the Ministerio de Ciencia e Innovacion (predoctoral FPI grant to A.R.).
dc.language Inglés es_ES
dc.publisher American Society for Microbiology es_ES
dc.relation.ispartof Molecular and Cellular Biology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Activated protein-kinase es_ES
dc.subject RNA POL-II es_ES
dc.subject Saccharomyces cerevisiae es_ES
dc.subject Gene expression es_ES
dc.subject Nuclear localization es_ES
dc.subject Transcription factor es_ES
dc.subject MAP kinase es_ES
dc.subject Environmental changes es_ES
dc.subject Histone Deacetylase es_ES
dc.subject Signaling pathways es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Deciphering dynamic dose responses of natural promoters and single cis elements upon osmotic and oxidative stress in yeast es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1128/MCB.00240-13
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BFU2011-23326/ES/REGULACION DE LA CROMATINA Y DE LA ESTRUCTURA MITOCONDRIAL EN RESPUESTA A ESTRES OSMOTICO/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Dolz Edo, L.; Rienzo, A.; Poveda Huertes, D.; Pascual-Ahuir Giner, MD.; Proft, MH. (2013). Deciphering dynamic dose responses of natural promoters and single cis elements upon osmotic and oxidative stress in yeast. Molecular and Cellular Biology. 33(11):2228-2240. https://doi.org/10.1128/MCB.00240-13 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1128/MCB.00240-13 es_ES
dc.description.upvformatpinicio 2228 es_ES
dc.description.upvformatpfin 2240 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 33 es_ES
dc.description.issue 11 es_ES
dc.relation.senia 259431 es_ES
dc.identifier.pmid 23530054
dc.identifier.pmcid PMC3648068 en_EN
dc.contributor.funder Ministerio de Ciencia e Innovación
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