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dc.contributor.author | García Hurtado, Noemi | es_ES |
dc.contributor.author | Carrera Bergua, Esther | es_ES |
dc.contributor.author | Ruiz Rivero, Omar José | es_ES |
dc.contributor.author | López Gresa, Mª Pilar | es_ES |
dc.contributor.author | Hedden, Peter | es_ES |
dc.contributor.author | Gong, Fan | es_ES |
dc.contributor.author | Garcia Martinez, Jose L | es_ES |
dc.date.accessioned | 2017-04-26T07:33:31Z | |
dc.date.available | 2017-04-26T07:33:31Z | |
dc.date.issued | 2012-10 | |
dc.identifier.issn | 0022-0957 | |
dc.identifier.uri | http://hdl.handle.net/10251/80000 | |
dc.description.abstract | [EN] Fruit-set and growth in tomato depend on the action of gibberellins (GAs). To evaluate the role of the GA biosynthetic enzyme GA 20-oxidase (GA20ox) in that process, the citrus gene CcGA20ox1 was overexpressed in tomato (Solanum lycopersicum L.) cv Micro-Tom. The transformed plants were taller, had non-serrated leaves, and some flowers displayed a protruding stigma due to a longer style, thus preventing self-pollination, similar to GA(3)-treated plants. Flowering was delayed compared with wild-type (WT) plants. Both yield and number of fruits per plant, some of them seedless, were higher in the transgenic plants. The Brix index value of fruit juice was also higher due to elevated citric acid content, but not glucose or fructose content. When emasculated, 1430% of ovaries from transgenic flowers developed parthenocarpically, whereas no parthenocarpy was found in emasculated WT flowers. The presence of early-13-hydroxylation and non-13-hydroxylation GA pathways was demonstrated in the shoot and fruit of Micro-Tom, as well as in two tall tomato cultivars (Ailsa Craig and UC-82). The transgenic plants had altered GA profiles containing higher concentrations of GA(4), from the non-13-hydroxylation pathway, which is generally a minor active GA in tomato. The effect of GA(4) application in enhancing stem growth and parthenocarpic fruit development was proportional to dose, with the same activity as GA(1). The results support the contention that GA20ox overexpression diverts GA metabolism from the early-13-hydroxylation pathway to the non-13-hydroxylation pathway. This led to enhanced GA(4) synthesis and higher yield, although the increase in GA(4) content in the ovary was not sufficient to induce full parthenocarpy. | es_ES |
dc.description.sponsorship | We thank Dr L. E. P. Peres for providing MT-D seeds, and Mrs T. Sabater for help with GA analysis. This work was supported by grants from the Ministerio de Ciencia y Tecnologia of Spain (BIO2006-13437 and BIO2009-07968). EC was the recipient of a Ramon y Cajal grant. Rothamsted Research is sponsored by the Biotechnology and Biological Sciences Research Council of the UK. | en_EN |
dc.language | Inglés | es_ES |
dc.publisher | Oxford University Press (OUP) | es_ES |
dc.relation.ispartof | Journal of Experimental Botany | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Fruit set | es_ES |
dc.subject | Gibberellin (GA) | es_ES |
dc.subject | Gibberellin 20-oxidase | es_ES |
dc.subject | Micro-Tom | es_ES |
dc.subject | Parthenocarpy | es_ES |
dc.subject | Tomato | es_ES |
dc.subject.classification | BIOQUIMICA Y BIOLOGIA MOLECULAR | es_ES |
dc.title | The characterization of transgenic tomato overexpressing gibberellin 20-oxidase reveals induction of parthenocarpic fruit growth, higher yield, and alteration of the gibberellin biosynthetic pathway | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1093/jxb/ers229 | |
dc.relation.projectID | info:eu-repo/grantAgreement/MEC//BIO2006-13437/ES/REGULACION HORMONAL DEL DESARROLLO REPRODUCTIVO: PAPEL DE LAS GIBERELINAS Y SU INTERACCION CON AUXINAS/ / | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//BIO2009-07968/ES/Control Hormonal De La Fructificacion En Tomate/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia | es_ES |
dc.description.bibliographicCitation | García Hurtado, N.; Carrera Bergua, E.; Ruiz Rivero, OJ.; López Gresa, MP.; Hedden, P.; Gong, F.; Garcia Martinez, JL. (2012). The characterization of transgenic tomato overexpressing gibberellin 20-oxidase reveals induction of parthenocarpic fruit growth, higher yield, and alteration of the gibberellin biosynthetic pathway. Journal of Experimental Botany. 63(16):5803-5813. doi:10.1093/jxb/ers229 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://doi.org/10.1093/jxb/ers229 | es_ES |
dc.description.upvformatpinicio | 5803 | es_ES |
dc.description.upvformatpfin | 5813 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 63 | es_ES |
dc.description.issue | 16 | es_ES |
dc.relation.senia | 231124 | es_ES |
dc.identifier.pmid | 22945942 | |
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