Muñoz Mayor, A.; Pineda Chaza, BJ.; García Abellán, JO.; Antón Martínez, MT.; García Sogo, B.; Sánchez Bel, P.; Flores, FB.... (2012). Overexpression. of dehydrin tas14 gene improves the osmotic stress imposed by drought and salinity in tomato. Journal of Plant Physiology. 169(5):459-468. https://doi.org/10.1016/j.jplph.2011.11.018
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/80646
Título:
|
Overexpression. of dehydrin tas14 gene improves the osmotic stress imposed by drought and salinity in tomato
|
Autor:
|
Muñoz Mayor, Alicia
Pineda Chaza, Benito José
García Abellán, José O.
Antón Martínez, María Teresa
García Sogo, Begoña
Sánchez Bel, Paloma
Flores, Francisco B.
Atarés Huerta, Alejandro
Angosto, Trinidad
Pintor Toro, José A.
Moreno Ferrero, Vicente
Bolarin, Maria C.
|
Entidad UPV:
|
Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes
Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural
|
Fecha difusión:
|
|
Resumen:
|
[EN] One strategy to increase the level of drought and salinity tolerance is the transfer of genes codifying different types of proteins functionally related to macromolecules protection, such as group 2 of late embryogenesis ...[+]
[EN] One strategy to increase the level of drought and salinity tolerance is the transfer of genes codifying different types of proteins functionally related to macromolecules protection, such as group 2 of late embryogenesis abundant (LEA) proteins or dehydrins. The TAS14 dehydrin was isolated and characterized in tomato and its expression was induced by osmotic stress (NaCl and mannitol) and abscisic acid (ABA) [Godoy et al., Plant Mol Biol 1994;26:1921-1934], yet its function in drought and salinity tolerance of tomato remains elusive. In this study, transgenic tomato plants overexpressing tas14 gene under the control of the 35SCaMV promoter were generated to assess the function of tas14 gene in drought and salinity tolerance. The plants overexpressing tas14 gene achieved improved long-term drought and salinity tolerance without affecting plant growth under non-stress conditions. A mechanism of osmotic stress tolerance via osmotic potential reduction and solutes accumulation, such as sugars and K+ is operating in tas14 overexpressing plants in drought conditions. A similar mechanism of osmotic stress tolerance was observed under salinity. Moreover, the overexpression of tas14 gene increased Na+ accumulation only in adult leaves, whereas in young leaves, the accumulated solutes were K+ and sugars, suggesting that plants overexpressing tas14 gene are able to distribute the Na+ accumulation between young and adult leaves over a prolonged period in stressful conditions. Measurement of ABA showed that the action mechanism of tas14 gene is associated with an earlier and greater accumulation of ABA in leaves during short-term periods. A good feature for the application of this gene in improving drought and salt stress tolerance is the fact that its constitutive expression does not affect plant growth under non-stress conditions, and tolerance induced by overexpression of tas14 gene was observed at the different stress degrees applied to the long term. (C) 2011 Elsevier GmbH. All rights reserved.
[-]
|
Palabras clave:
|
Drought tolerance
,
Salinity tolerance
,
Tomato
,
Solanum lycopersicum
,
tas14 gene
,
Osmotic stress
|
Derechos de uso:
|
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
|
Fuente:
|
Journal of Plant Physiology. (issn:
0176-1617
)
|
DOI:
|
10.1016/j.jplph.2011.11.018
|
Editorial:
|
Elsevier
|
Versión del editor:
|
http://doi.org/10.1016/j.jplph.2011.11.018
|
Código del Proyecto:
|
info:eu-repo/grantAgreement/MICINN//AGL2009-13388-C03-02/ES/Caracterizacion De Mutantes De Insercion Y Analisis Funcional De Genes Que Controlan El Tamaño Del Fruto De Tomate/
info:eu-repo/grantAgreement/MICINN//AGL2009-13388-C03-03/ES/Caracterizacion De Mutantes De Insercion Y Analisis Funcional De Genes Relacionados Con Tolerancia A La Salinidad Y Estres Hidrico En Tomate Cultivado Y Silvestre/
info:eu-repo/grantAgreement/f SéNeCa/Funding Program for Research Groups of Excellence/04553%2FGERM%2F06/
info:eu-repo/grantAgreement/MICINN//AGL2009-13388-C03-01/ES/Generacion De Mutantes De Insercion De Tomate Cultivado Y Silvestre E Identificacion De Genes Implicados En Procesos Del Desarrollo Y Tolerancia A Estres Abiotico/
|
Agradecimientos:
|
This work was supported by the Spanish Ministry of Science and Innovation through grant AGL2009-13388-C03 and by the Council of Science and Technology from the Region of Murcia (Spain) (Fundacion SENECA) through grant ...[+]
This work was supported by the Spanish Ministry of Science and Innovation through grant AGL2009-13388-C03 and by the Council of Science and Technology from the Region of Murcia (Spain) (Fundacion SENECA) through grant 04553/GERM/06.
[-]
|
Tipo:
|
Artículo
|