Muñoz Bertomeu, J.; Cascales Miñana, B.; Mulet Salort, JM.; Baroja-Fernandez, E.; Pozueta Romero, J.; Kuhn, JM.; Segura Garcia Del Rio, J.... (2009). Plastidial Glyceraldehyde-3-Phosphate Dehydrogenase Deficiency Leads to Altered Root Development and Affects the Sugar and Amino Acid Balance in Arabidopsis. Plant Physiology. 151(2):541-558. https://doi.org/10.1104/pp.109.143701
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/80703
Title:
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Plastidial Glyceraldehyde-3-Phosphate Dehydrogenase Deficiency Leads to Altered Root Development and Affects the Sugar and Amino Acid Balance in Arabidopsis
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Author:
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Muñoz Bertomeu, Jesús
Cascales Miñana, Borja
Mulet Salort, José Miguel
Baroja-Fernandez, Edurne
Pozueta Romero, Javier
Kuhn, Josef M.
Segura Garcia del Rio, Juan
Ros Palau, Roc
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UPV Unit:
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Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural
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Issued date:
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Abstract:
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[EN] Glycolysis is a central metabolic pathway that, in plants, occurs in both the cytosol and the plastids. The glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the conversion of glyceraldehyde-3-phosphate ...[+]
[EN] Glycolysis is a central metabolic pathway that, in plants, occurs in both the cytosol and the plastids. The glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate with concomitant reduction of NAD(+) to NADH. Both cytosolic (GAPCs) and plastidial (GAPCps) GAPDH activities have been described. However, the in vivo functions of the plastidial isoforms remain unresolved. In this work, we have identified two Arabidopsis (Arabidopsis thaliana) chloroplast/plastid-localized GAPDH isoforms (GAPCp1 and GAPCp2). gapcp double mutants display a drastic phenotype of arrested root development, dwarfism, and sterility. In spite of their low gene expression level as compared with other GAPDHs, GAPCp down-regulation leads to altered gene expression and to drastic changes in the sugar and amino acid balance of the plant. We demonstrate that GAPCps are important for the synthesis of serine in roots. Serine supplementation to the growth medium rescues root developmental arrest and restores normal levels of carbohydrates and sugar biosynthetic activities in gapcp double mutants. We provide evidence that the phosphorylated pathway of Ser biosynthesis plays an important role in supplying serine to roots. Overall, these studies provide insights into the in vivo functions of the GAPCps in plants. Our results emphasize the importance of the plastidial glycolytic pathway, and specifically of GAPCps, in plant primary metabolism.
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Subjects:
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In-source leaves
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Serine biosynthesis
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Molecular characterization
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Escherichia-Coli
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Oxidative stress
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Gene-expression
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Crucial role
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Thaliana
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Metabolism
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Pathway
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Copyrigths:
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Reserva de todos los derechos
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Source:
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Plant Physiology. (issn:
0032-0889
)
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DOI:
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10.1104/pp.109.143701
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Publisher:
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American Society of Plant Biologists
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Publisher version:
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https://doi.org/10.1104/pp.109.143701
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Project ID:
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info:eu-repo/grantAgreement/NSF/Directorate for Biological Sciences/0417118/US/
info:eu-repo/grantAgreement/EC/FP6/509278/EU/Involvement of RNA-binding Proteins in the plant responses to drought and salinity/SALT-DROUGHT/
info:eu-repo/grantAgreement/MEC//BFU2006-01621/ES/CARACTERIZACION FUNCIONAL DE LAS PROTEINAS DE UNION A RNA ABH1 Y SATO1 DE ARABIDOPSIS. RELACION CON LOS ESTRESES HIDRICO Y SALINO/
info:eu-repo/grantAgreement/Generalitat Valenciana//ACOMP%2F2009%2F328/ES/CARACTERIZACION FUNCIONAL DE LAS PROTEINAS DE UNION A RNA ABH1 Y SATO. RELACION CON LOS ESTRESES HIDRICO Y SALINO/
info:eu-repo/grantAgreement/Generalitat Valenciana//PROMETEO09%2F2009%2F075/ES/PROMETEO09%2F2009%2F075/
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Thanks:
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This work was supported by the European Union (Sixth Framework programme, grant no. MOIF-CT-2004-50927), by the Spanish Government (grant no. BFU2006-01621/BFI), by the Valencian Government (grant nos. PROMETEO/2009/075 ...[+]
This work was supported by the European Union (Sixth Framework programme, grant no. MOIF-CT-2004-50927), by the Spanish Government (grant no. BFU2006-01621/BFI), by the Valencian Government (grant nos. PROMETEO/2009/075 and ACOMP/2009/328), by a Formacion de Profesorado Universitario research fellowship from the Spanish Government to B. C.-M., and by the National Institutes of Health and the National Science Foundation (grant nos. GM060396 and MCB0417118, respectively, to Julian Schroeder at University of California San Diego).
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Type:
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Artículo
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