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dc.contributor.author | Alvarez-Ponce, David | es_ES |
dc.contributor.author | Sabater-Munoz, Beatriz | es_ES |
dc.contributor.author | Toft, Chirstina | es_ES |
dc.contributor.author | Ruíz-González, Mario Javier | es_ES |
dc.contributor.author | Fares Riaño, Mario Ali | es_ES |
dc.date.accessioned | 2017-05-18T09:32:29Z | |
dc.date.available | 2017-05-18T09:32:29Z | |
dc.date.issued | 2016-09 | |
dc.identifier.issn | 1759-6653 | |
dc.identifier.uri | http://hdl.handle.net/10251/81366 | |
dc.description.abstract | [EN] The Neutral Theory of Molecular Evolution is considered the most powerful theory to understand the evolutionary behavior of proteins. One of the main predictions of this theory is that essential proteins should evolve slower than dispensable ones owing to increased selective constraints. Comparison of genomes of different species, however, has revealed only small differences between the rates of evolution ofessential and nonessential proteins. In some analyses, these differences vanish once confounding factors are controlled for, whereas in other cases essentiality seems to have an independent, albeit small, effect. It has been argued that comparing relatively distant genomes may entail a number of limitations. For instance, many of the genes that are dispensable in controlled lab conditions may be essential in some of the conditions faced in nature. Moreover, essentiality can change during evolution, and rates of protein evolution are simultaneously shaped by a variety of factors, whose individual effects are difficult to isolate. Here, we conducted two parallel mutation accumulation experiments in Escherichia coli, during 5,500–5,750 generations, and compared the genomes at different points of the experiments. Our approach (a short-term experiment, under highly controlled conditions) enabled us to overcome many of the limitations of previous studies. We observed that essential proteins evolved substantially slower than nonessential ones during our experiments. Strikingly, rates of protein evolution were only moderately affected by expression level and protein length. | es_ES |
dc.description.sponsorship | We are grateful to Kais Fares, Maria Prats-Escriche and Victor Berlanga-Laparra for technical assistance with the evolution experiments. We are also grateful to the Editor and two anonymous referees for helpful comments. This work was supported by grants from the Spanish Ministerio de Economia y Competitividad (BFU2009-12022, BFU2012-36346, and BFU2015-66073-P) and Science Foundation Ireland (12/IP/1673) to M.A.F. D.A.-P. was partially supported by funds from the University of Nevada, Reno. C.T. was supported by a European Molecular Biology Organization long-term fellowship (EMBO ALTF 730-2011) and a Juan de la Cierva fellowship from the Ministerio de Economia y Competitividad (JCA-2012-14056). M.X.R.-G. was partially supported by a JAE DOC fellowship from the Ministerio de Economia y Competitividad, Spain. | |
dc.language | Inglés | es_ES |
dc.publisher | Oxford University Press (OUP) | es_ES |
dc.relation.ispartof | Genome Biology and Evolution | es_ES |
dc.rights | Reconocimiento - No comercial (by-nc) | es_ES |
dc.subject | Essentiality | es_ES |
dc.subject | Rates of evolution | es_ES |
dc.subject | dN/dS | es_ES |
dc.subject | Experimental evolution | es_ES |
dc.subject | Neutral theory | es_ES |
dc.title | Essentiality Is a Strong Determinant of Protein Rates of Evolution during Mutation Accumulation Experiments in Escherichia coli | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1093/gbe/evw205 | |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//BFU2009-12022/ES/Impacto De La Duplicacion Genomica En La Innovacion Y Geometria Funcional De Arabidopsis Thaliana/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/SFI/SFI Investigator Programme/12%2FIP%2F1673/IE/Understanding the role of molecular chaperones in robustness and functional innovation/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//BFU2012-36346/ES/EL PAPEL DE LA DUPLICACION GENICA EN LA COMPLEJIDAD DE SISTEMAS BIOLOGICOS: RE-DIRECCION DE DINAMICAS MUTACIONALES Y ORIGEN DE INNOVACIONES BIOLOGICAS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//BFU2015-66073-P/ES/CARACTERIZANDO LOS MECANISMOS DE INNOVACION POR DUPLICACION GENICA/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EMBO//ALTF 730-2011/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//JCI-2012-14056/ES/JCI-2012-14056/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | Alvarez-Ponce, D.; Sabater-Munoz, B.; Toft, C.; Ruíz-González, MJ.; Fares Riaño, MA. (2016). Essentiality Is a Strong Determinant of Protein Rates of Evolution during Mutation Accumulation Experiments in Escherichia coli. Genome Biology and Evolution. 8(9):2914-2927. https://doi.org/10.1093/gbe/evw205 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1093/gbe/evw205 | es_ES |
dc.description.upvformatpinicio | 2914 | es_ES |
dc.description.upvformatpfin | 2927 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 8 | es_ES |
dc.description.issue | 9 | es_ES |
dc.relation.senia | 332052 | es_ES |
dc.identifier.pmid | 27566759 | |
dc.identifier.pmcid | PMC5630975 | |
dc.contributor.funder | Ministerio de Economía y Competitividad | |
dc.contributor.funder | Science Foundation Ireland | |
dc.contributor.funder | European Molecular Biology Organization | |
dc.contributor.funder | Ministerio de Ciencia e Innovación |