- -

Essentiality Is a Strong Determinant of Protein Rates of Evolution during Mutation Accumulation Experiments in Escherichia coli.

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

Essentiality Is a Strong Determinant of Protein Rates of Evolution during Mutation Accumulation Experiments in Escherichia coli.

Show simple item record

Files in this item

dc.contributor.author Alvarez-Ponce, David es_ES
dc.contributor.author Sabater-Munoz, Beatriz es_ES
dc.contributor.author Toft, Chirstina es_ES
dc.contributor.author Ruíz-González, Mario Javier es_ES
dc.contributor.author Fares Riaño, Mario Ali es_ES
dc.date.accessioned 2017-05-18T09:32:29Z
dc.date.available 2017-05-18T09:32:29Z
dc.date.issued 2016-09
dc.identifier.issn 1759-6653
dc.identifier.uri http://hdl.handle.net/10251/81366
dc.description.abstract [EN] The Neutral Theory of Molecular Evolution is considered the most powerful theory to understand the evolutionary behavior of proteins. One of the main predictions of this theory is that essential proteins should evolve slower than dispensable ones owing to increased selective constraints. Comparison of genomes of different species, however, has revealed only small differences between the rates of evolution ofessential and nonessential proteins. In some analyses, these differences vanish once confounding factors are controlled for, whereas in other cases essentiality seems to have an independent, albeit small, effect. It has been argued that comparing relatively distant genomes may entail a number of limitations. For instance, many of the genes that are dispensable in controlled lab conditions may be essential in some of the conditions faced in nature. Moreover, essentiality can change during evolution, and rates of protein evolution are simultaneously shaped by a variety of factors, whose individual effects are difficult to isolate. Here, we conducted two parallel mutation accumulation experiments in Escherichia coli, during 5,500–5,750 generations, and compared the genomes at different points of the experiments. Our approach (a short-term experiment, under highly controlled conditions) enabled us to overcome many of the limitations of previous studies. We observed that essential proteins evolved substantially slower than nonessential ones during our experiments. Strikingly, rates of protein evolution were only moderately affected by expression level and protein length. es_ES
dc.description.sponsorship We are grateful to Kais Fares, Maria Prats-Escriche and Victor Berlanga-Laparra for technical assistance with the evolution experiments. We are also grateful to the Editor and two anonymous referees for helpful comments. This work was supported by grants from the Spanish Ministerio de Economia y Competitividad (BFU2009-12022, BFU2012-36346, and BFU2015-66073-P) and Science Foundation Ireland (12/IP/1673) to M.A.F. D.A.-P. was partially supported by funds from the University of Nevada, Reno. C.T. was supported by a European Molecular Biology Organization long-term fellowship (EMBO ALTF 730-2011) and a Juan de la Cierva fellowship from the Ministerio de Economia y Competitividad (JCA-2012-14056). M.X.R.-G. was partially supported by a JAE DOC fellowship from the Ministerio de Economia y Competitividad, Spain.
dc.language Inglés es_ES
dc.publisher Oxford University Press (OUP) es_ES
dc.relation MINECO/BFU2009-12022 es_ES
dc.relation MINECO/BFU2012-36346 es_ES
dc.relation MINECO/BFU2015-66073-P es_ES
dc.relation SFI/12/IP/1673 es_ES
dc.relation EMBO/ALTF 730-2011 es_ES
dc.relation MINECO/JCA-2012-14056 es_ES
dc.relation.ispartof Genome Biology and Evolution es_ES
dc.rights Reconocimiento - No comercial (by-nc) es_ES
dc.subject Essentiality es_ES
dc.subject Rates of evolution es_ES
dc.subject dN/dS es_ES
dc.subject Experimental evolution es_ES
dc.subject Neutral theory es_ES
dc.title Essentiality Is a Strong Determinant of Protein Rates of Evolution during Mutation Accumulation Experiments in Escherichia coli. es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1093/gbe/evw205
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Alvarez-Ponce, D.; Sabater-Munoz, B.; Toft, C.; Ruíz-González, MJ.; Fares Riaño, MA. (2016). Essentiality Is a Strong Determinant of Protein Rates of Evolution during Mutation Accumulation Experiments in Escherichia coli. Genome Biology and Evolution. 8(9):2914-2927. doi:10.1093/gbe/evw205 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1093/gbe/evw205 es_ES
dc.description.upvformatpinicio 2914 es_ES
dc.description.upvformatpfin 2927 es_ES
dc.type.version info:eu repo/semantics/publishedVersion es_ES
dc.description.volume 8 es_ES
dc.description.issue 9 es_ES
dc.relation.senia 332052 es_ES
dc.identifier.pmid 27566759
dc.identifier.pmcid PMC5630975
dc.contributor.funder Ministerio de Economía y Competitividad
dc.contributor.funder Science Foundation Ireland
dc.contributor.funder European Molecular Biology Organization


This item appears in the following Collection(s)

Show simple item record