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dc.contributor.author | Peris Chanzá, Ernesto José | es_ES |
dc.contributor.author | Bañuls Polo, María-José | es_ES |
dc.contributor.author | Puchades, Rosa | es_ES |
dc.contributor.author | Maquieira Catala, Ángel | es_ES |
dc.date.accessioned | 2017-05-29T11:42:00Z | |
dc.date.available | 2017-05-29T11:42:00Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 2050-750X | |
dc.identifier.uri | http://hdl.handle.net/10251/81921 | |
dc.description.abstract | [EN] A methodology to modify Blu-ray disk (BD) surfaces by spin-coating an SU-8 epoxy photoresist is evaluated, to create a hybrid material with new functionalities. The optical performance of the new film surface disk is analyzed. The light-mediated activation of epoxy-to-thiol chemistry is applied to the site-specific and covalent binding of thiol-ended oligonucleotide probes to the SU-8 disk surface. For the optimized hybridization conditions, a limit of detection of 1 pM is reached employing enzymatic development. PCR products, from a Salmonella typhimurium serotype, are detected at an amplicon concentration of 20 pM. The reading of the assays is performed using a commercial BD-player. | es_ES |
dc.description.sponsorship | This work has been funded by the projects FEDER CTQ2010-15943 (CICYT, Spain), PROMETEO 2010/008 (Generalitat Valenciana) and an interdisciplinary UPV PAID 05 - 10 grant. | en_EN |
dc.language | Inglés | es_ES |
dc.publisher | Royal Society of Chemistry | es_ES |
dc.relation.ispartof | Journal of Materials Chemistry B | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Compact-disk | es_ES |
dc.subject | Immobilization | es_ES |
dc.subject | Microarrays | es_ES |
dc.subject | Biomolecules | es_ES |
dc.subject | Photoresist | es_ES |
dc.subject | Spectroscopy | es_ES |
dc.subject | Fabrication | es_ES |
dc.subject | Expression | es_ES |
dc.subject | Chemistry | es_ES |
dc.subject | Protein | es_ES |
dc.subject.classification | QUIMICA ANALITICA | es_ES |
dc.title | Photoattachment of thiolated DNA probes on SU-8 spin-coated Blu-ray disk surfaces for biosensing | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1039/c3tb21026j | |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//CTQ2010-15943/ES/ESTUDIO DE NUEVAS VIAS DE DESARROLLO DE BIOMEMS PARA SCREENING MASIVO. DEMOSTRACION DE CONCEPTO COMO HERRAMIENTA DE ANALISIS APLICABLE EN "OMICAS"./ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEO%2F2010%2F008/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic | es_ES |
dc.description.bibliographicCitation | Peris Chanzá, EJ.; Bañuls Polo, M.; Puchades, R.; Maquieira Catala, Á. (2013). Photoattachment of thiolated DNA probes on SU-8 spin-coated Blu-ray disk surfaces for biosensing. Journal of Materials Chemistry B. 1(45):6245-6253. https://doi.org/10.1039/c3tb21026j | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://doi.org/10.1039/c3tb21026j | es_ES |
dc.description.upvformatpinicio | 6245 | es_ES |
dc.description.upvformatpfin | 6253 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 1 | es_ES |
dc.description.issue | 45 | es_ES |
dc.relation.senia | 252282 | es_ES |
dc.identifier.eissn | 2050-7518 | |
dc.contributor.funder | Ministerio de Ciencia e Innovación | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |