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dc.contributor.author | Molina Serrano, Diego | es_ES |
dc.contributor.author | Marques Signes, Jorge | es_ES |
dc.contributor.author | Nohales Zafra, Maria Angeles | es_ES |
dc.contributor.author | Flores Pedauye, Ricardo | es_ES |
dc.contributor.author | Daros Arnau, Jose Antonio | es_ES |
dc.date.accessioned | 2017-06-09T11:08:34Z | |
dc.date.available | 2017-06-09T11:08:34Z | |
dc.date.issued | 2012-03 | |
dc.identifier.issn | 1547-6286 | |
dc.identifier.uri | http://hdl.handle.net/10251/82649 | |
dc.description.abstract | [EN] Bacteria and archaea contain a 2'-5' RNA ligase that seals in vitro 2',3'-cyclic phosphodiester and 5'-hydroxyl RNA termini, generating a 2',5'-phosphodiester bond. In our search for an RNA ligase able to circularize the monomeric linear replication intermediates of viroids belonging to the family Avsunviroidae, which replicate in the chloroplast, we have identified in spinach (Spinacea oleracea L.) chloroplasts a new RNA ligase activity whose properties resemble those of the bacterial and archaeal 2'-5' RNA ligase. The spinach chloroplastic RNA ligase recognizes the 5'-hydroxyl and 2',3'-cyclic phosphodiester termini of Avocado sunblotch viroid and Eggplant latent viroid RNAs produced by hammerhead-mediated self-cleavage, yielding circular products linked through an atypical, most likely 2',5'-phosphodiester, bond. The enzyme neither requires divalent cations as cofactors, nor NTPs as substrate. The reaction apparently reaches equilibrium at a low ratio between the final circular product and the linear initial substrate. Even if its involvement in viroid replication seems unlikely, the identification of a 2'-5' RNA ligase activity in higher plant chloroplasts, with properties very similar to an analogous enzyme widely distributed in bacterial and archaeal proteomes, is intriguing and suggests an important biological role so far unknown. | es_ES |
dc.description.sponsorship | This work was supported by grants BIO2008-01986 and BFU2008-03154 from Ministerio de Ciencia e Innovacion (MICINN) (Spain) and BFU2005-06808 from Ministerio de Educacion y Ciencia (MEC) (Spain). D.M.S. was the recipient of a predoctoral fellowship from Ministerio de Ciencia y Tecnologia (Spain), and J.M. and M.A.N. from MEC. | en_EN |
dc.language | Inglés | es_ES |
dc.publisher | Taylor & Francis: STM, Behavioural Science and Public Health Titles | es_ES |
dc.relation.ispartof | RNA Biology | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | RNA ligase | es_ES |
dc.subject | 2'-phosphodiester bond | es_ES |
dc.subject | 5'-phosphodiester bond | es_ES |
dc.subject | Circular RNA | es_ES |
dc.subject | Bacterial and archaeal 2 '-5 ' RNA ligase | es_ES |
dc.subject | V | es_ES |
dc.title | A chloroplastic RNA ligase activity analogous to the bacterial and archaeal 2 '-5 ' RNA ligase | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.4161/rna.19218 | |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//BIO2008-01986/ES/INTERACCIONES RNA-PROTEINA EN EL CICLO INFECCIOSO DE PATOGENOS DE RNA DE PLANTAS/ / | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MEC//BFU2005-06808/ES/RNAS VIROIDALES: INTERACCIONES CON SUS HUESPEDES IMPLICADAS EN REPLICACION Y PATOGENESIS, Y DESARROLLO DE HERRAMIENTAS BIOTECNOLOGICAS PARA SU CONTROL/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//BFU2008-03154/ES/INTERACCIONES VIROIDE-HUESPED: PAPEL DE LAS RIBOZIMAS, DEL SILENCIAMIENTO MEDIADO POR RNA, Y DE LA RECOMBINACION DE RNA/ | es_ES |
dc.rights.accessRights | Cerrado | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | Molina Serrano, D.; Marques Signes, J.; Nohales Zafra, MA.; Flores Pedauye, R.; Daros Arnau, JA. (2012). A chloroplastic RNA ligase activity analogous to the bacterial and archaeal 2 '-5 ' RNA ligase. RNA Biology. 9(3):326-333. https://doi.org/10.4161/rna.19218 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://doi.org/10.4161/rna.19218 | es_ES |
dc.description.upvformatpinicio | 326 | es_ES |
dc.description.upvformatpfin | 333 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 9 | es_ES |
dc.description.issue | 3 | es_ES |
dc.relation.senia | 232562 | es_ES |
dc.identifier.pmid | 22336712 | |
dc.contributor.funder | Ministerio de Ciencia e Innovación | es_ES |
dc.contributor.funder | Ministerio de Educación y Ciencia | es_ES |
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