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A chloroplastic RNA ligase activity analogous to the bacterial and archaeal 2 '-5 ' RNA ligase

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A chloroplastic RNA ligase activity analogous to the bacterial and archaeal 2 '-5 ' RNA ligase

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dc.contributor.author Molina Serrano, Diego es_ES
dc.contributor.author Marques Signes, Jorge es_ES
dc.contributor.author Nohales Zafra, Maria Angeles es_ES
dc.contributor.author Flores Pedauye, Ricardo es_ES
dc.contributor.author Daros Arnau, Jose Antonio es_ES
dc.date.accessioned 2017-06-09T11:08:34Z
dc.date.available 2017-06-09T11:08:34Z
dc.date.issued 2012-03
dc.identifier.issn 1547-6286
dc.identifier.uri http://hdl.handle.net/10251/82649
dc.description.abstract [EN] Bacteria and archaea contain a 2'-5' RNA ligase that seals in vitro 2',3'-cyclic phosphodiester and 5'-hydroxyl RNA termini, generating a 2',5'-phosphodiester bond. In our search for an RNA ligase able to circularize the monomeric linear replication intermediates of viroids belonging to the family Avsunviroidae, which replicate in the chloroplast, we have identified in spinach (Spinacea oleracea L.) chloroplasts a new RNA ligase activity whose properties resemble those of the bacterial and archaeal 2'-5' RNA ligase. The spinach chloroplastic RNA ligase recognizes the 5'-hydroxyl and 2',3'-cyclic phosphodiester termini of Avocado sunblotch viroid and Eggplant latent viroid RNAs produced by hammerhead-mediated self-cleavage, yielding circular products linked through an atypical, most likely 2',5'-phosphodiester, bond. The enzyme neither requires divalent cations as cofactors, nor NTPs as substrate. The reaction apparently reaches equilibrium at a low ratio between the final circular product and the linear initial substrate. Even if its involvement in viroid replication seems unlikely, the identification of a 2'-5' RNA ligase activity in higher plant chloroplasts, with properties very similar to an analogous enzyme widely distributed in bacterial and archaeal proteomes, is intriguing and suggests an important biological role so far unknown. es_ES
dc.description.sponsorship This work was supported by grants BIO2008-01986 and BFU2008-03154 from Ministerio de Ciencia e Innovacion (MICINN) (Spain) and BFU2005-06808 from Ministerio de Educacion y Ciencia (MEC) (Spain). D.M.S. was the recipient of a predoctoral fellowship from Ministerio de Ciencia y Tecnologia (Spain), and J.M. and M.A.N. from MEC. en_EN
dc.language Inglés es_ES
dc.publisher Taylor & Francis: STM, Behavioural Science and Public Health Titles es_ES
dc.relation Ministerio de Ciencia e Innovación, Spain [BIO2008-01986] [BFU2008-03154] es_ES
dc.relation Ministerio de Educación y Ciencia, Spain [BFU2005-06808] es_ES
dc.relation.ispartof RNA Biology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject RNA ligase es_ES
dc.subject 2'-phosphodiester bond es_ES
dc.subject 5'-phosphodiester bond es_ES
dc.subject Circular RNA es_ES
dc.subject Bacterial and archaeal 2 '-5 ' RNA ligase es_ES
dc.subject V es_ES
dc.title A chloroplastic RNA ligase activity analogous to the bacterial and archaeal 2 '-5 ' RNA ligase es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.4161/rna.19218
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Molina Serrano, D.; Marques Signes, J.; Nohales Zafra, MA.; Flores Pedauye, R.; Daros Arnau, JA. (2012). A chloroplastic RNA ligase activity analogous to the bacterial and archaeal 2 '-5 ' RNA ligase. RNA Biology. 9(3):326-333. doi:10.4161/rna.19218 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://doi.org/10.4161/rna.19218 es_ES
dc.description.upvformatpinicio 326 es_ES
dc.description.upvformatpfin 333 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 9 es_ES
dc.description.issue 3 es_ES
dc.relation.senia 232562 es_ES
dc.identifier.pmid 22336712


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