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The use of a real-time luciferase assay to quantify gene expression dynamics in the living yeast cell

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The use of a real-time luciferase assay to quantify gene expression dynamics in the living yeast cell

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Rienzo, A.; Pascual-Ahuir Giner, MD.; Proft, MH. (2012). The use of a real-time luciferase assay to quantify gene expression dynamics in the living yeast cell. Yeast. 29(6):219-231. doi:10.1002/yea.2905

Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/82837

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Title: The use of a real-time luciferase assay to quantify gene expression dynamics in the living yeast cell
Author:
UPV Unit: Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes
Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural
Issued date:
Abstract:
[EN] A destabilized version of firefly luciferase was used in living yeast cells as a real-time reporter for gene expression. This highly sensitive and non-invasive system can be simultaneously used upon many different ...[+]
Subjects: Luciferase , Gene expression , Dose-response , Promoter , Saccharomyces cerevisiae
Copyrigths: Cerrado
Source:
Yeast. (issn: 0749-503X )
DOI: 10.1002/yea.2905
Publisher:
Wiley
Publisher version: http://doi.org/10.1002/yea.2905
Thanks:
We thank Takayoshi Kuno for the kind gift of plasmid pGL3, containing the destabilized firefly luciferase gene. This study was supported by the Ministerio de Ciencia e Innovacion (Grant Nos BFU2008-00271 and BFU2011-23326). ...[+]
Type: Artículo

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