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Construction and analysis of full-length and normalized cDNA libraries from citrus

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Construction and analysis of full-length and normalized cDNA libraries from citrus

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Marques, MC.; Perez Amador, MA. (2012). Construction and analysis of full-length and normalized cDNA libraries from citrus. Functional Genomics: Methods and Protocols. 815:51-65. https://doi.org/10.1007/978-1-61779-424-7_5

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Título: Construction and analysis of full-length and normalized cDNA libraries from citrus
Autor: Marques, M. Carmen Perez Amador, Miguel Angel
Entidad UPV: Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes
Fecha difusión:
Resumen:
[EN] We have developed an integrated method to generate a normalized cDNA collection enriched in full-length and rare transcripts from citrus, using different species and multiple tissues and developmental stages. Interpretation ...[+]
Palabras clave: Library , cDNA , Citrus , Full-length , Normalized , SMART , DSN nuclease , Gateway technology
Derechos de uso: Reserva de todos los derechos
ISBN: 978-1-61779-423-0 978-1-61779-424-7
Fuente:
Functional Genomics: Methods and Protocols. (issn: 1064-3745 )
DOI: 10.1007/978-1-61779-424-7_5
Editorial:
Springer New York
Versión del editor: https://doi.org/10.1007/978-1-61779-424-7_5
Serie: Methods in Molecular Biology;815
Código del Proyecto:
info:eu-repo/grantAgreement/MICYT//GEN2001-4885-C05-01/ES/ESTUDIO DE LA RESPUESTA A ESTRESES BIOTICOS DE CITRICOS MEDIANTE GENOMICA FUNCIONAL/
info:eu-repo/grantAgreement/MICYT//GEN2001-4885-C05-02/ES/ESTUDIO DEL DESARROLLO VEGETATIVO Y REPRODUCTIVO DE CITRICOS MEDIANTE GENOMICA FUNCIONAL/
Agradecimientos:
The authors would like to thank to all participants in the Spanish Citrus Functional Genomic Project, specially to Drs. Javier Forment, Jose Gadea, and Vicente Conejero. This work was funded by grants from the Spanish ...[+]
Tipo: Artículo Capítulo de libro

References

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