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Construction and analysis of full-length and normalized cDNA libraries from citrus

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Construction and analysis of full-length and normalized cDNA libraries from citrus

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Marques, MC.; Perez Amador, MA. (2012). Construction and analysis of full-length and normalized cDNA libraries from citrus. Functional Genomics: Methods and Protocols. 815:51-65. doi:10.1007/978-1-61779-424-7_5

Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/83428

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Title: Construction and analysis of full-length and normalized cDNA libraries from citrus
Author:
UPV Unit: Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes
Issued date:
Abstract:
[EN] We have developed an integrated method to generate a normalized cDNA collection enriched in full-length and rare transcripts from citrus, using different species and multiple tissues and developmental stages. Interpretation ...[+]
Subjects: Library , cDNA , Citrus , Full-length , Normalized , SMART , DSN nuclease , Gateway technology
Copyrigths: Reserva de todos los derechos
ISBN: 978-1-61779-423-0 978-1-61779-424-7
Source:
Functional Genomics: Methods and Protocols. (issn: 1064-3745 )
DOI: 10.1007/978-1-61779-424-7_5
Publisher:
Springer New York
Publisher version: https://doi.org/10.1007/978-1-61779-424-7_5
Series: Methods in Molecular Biology;815
Thanks:
The authors would like to thank to all participants in the Spanish Citrus Functional Genomic Project, specially to Drs. Javier Forment, Jose Gadea, and Vicente Conejero. This work was funded by grants from the Spanish ...[+]
Type: Artículo Capítulo de libro

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