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Construction and analysis of full-length and normalized cDNA libraries from citrus

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Construction and analysis of full-length and normalized cDNA libraries from citrus

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dc.contributor.author Marques, M. Carmen es_ES
dc.contributor.author Perez Amador, Miguel Angel es_ES
dc.date.accessioned 2017-06-22T09:36:19Z
dc.date.available 2017-06-22T09:36:19Z
dc.date.issued 2012
dc.identifier.isbn 978-1-61779-423-0
dc.identifier.isbn 978-1-61779-424-7
dc.identifier.issn 1064-3745
dc.identifier.uri http://hdl.handle.net/10251/83428
dc.description.abstract [EN] We have developed an integrated method to generate a normalized cDNA collection enriched in full-length and rare transcripts from citrus, using different species and multiple tissues and developmental stages. Interpretation of ever-increasing raw sequence information generated by modern genome sequencing technologies faces multiple challenges, such as gene function analysis and genome annotation. In this regard, the availability of full-length cDNA clones facilitates functional analysis of the corresponding genes enabling manipulation of their expression and the generation of a variety of tagged versions of the native protein. The development of full-length cDNA sequences has the power to improve the quality of genome annotation, as well as provide tools for functional characterization of genes es_ES
dc.description.sponsorship The authors would like to thank to all participants in the Spanish Citrus Functional Genomic Project, specially to Drs. Javier Forment, Jose Gadea, and Vicente Conejero. This work was funded by grants from the Spanish Government GEN2001-4885-CO5-01 and GEN2001-4885-CO5-02.
dc.language Inglés es_ES
dc.publisher Springer New York es_ES
dc.relation.ispartof Functional Genomics: Methods and Protocols es_ES
dc.relation.ispartofseries Methods in Molecular Biology;815
dc.rights Reserva de todos los derechos es_ES
dc.subject Library es_ES
dc.subject cDNA es_ES
dc.subject Citrus es_ES
dc.subject Full-length es_ES
dc.subject Normalized es_ES
dc.subject SMART es_ES
dc.subject DSN nuclease es_ES
dc.subject Gateway technology es_ES
dc.title Construction and analysis of full-length and normalized cDNA libraries from citrus es_ES
dc.type Artículo es_ES
dc.type Capítulo de libro es_ES
dc.identifier.doi 10.1007/978-1-61779-424-7_5
dc.relation.projectID info:eu-repo/grantAgreement/MICYT//GEN2001-4885-C05-01/ES/ESTUDIO DE LA RESPUESTA A ESTRESES BIOTICOS DE CITRICOS MEDIANTE GENOMICA FUNCIONAL/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICYT//GEN2001-4885-C05-02/ES/ESTUDIO DEL DESARROLLO VEGETATIVO Y REPRODUCTIVO DE CITRICOS MEDIANTE GENOMICA FUNCIONAL/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Marques, MC.; Perez Amador, MA. (2012). Construction and analysis of full-length and normalized cDNA libraries from citrus. Functional Genomics: Methods and Protocols. 815:51-65. https://doi.org/10.1007/978-1-61779-424-7_5 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1007/978-1-61779-424-7_5 es_ES
dc.description.upvformatpinicio 51 es_ES
dc.description.upvformatpfin 65 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 815 es_ES
dc.relation.senia 232697 es_ES
dc.identifier.pmid 22130983
dc.contributor.funder Ministerio de Ciencia y Tecnología
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