Catalá, M.; Gasulla Vidal, F.; Pradas Del Real, AE.; García-Breijo, F.; Reig Armiñana, J.; Barreno Rodriguez, E. (2010). Fungal-associated NO is involved in the regulation of oxidative stress during rehydration in lichen symbiosis. BMC Microbiology. 10. https://doi.org/10.1186/1471-2180-10-297
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/84978
Título:
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Fungal-associated NO is involved in the regulation of oxidative stress during rehydration in lichen symbiosis
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Autor:
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Catalá, Myriam
Gasulla Vidal, Francisco
Pradas del Real, Ana Elena
García-Breijo, Francisco-José
Reig Armiñana, José
Barreno Rodriguez, Eva
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Entidad UPV:
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Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural
Universitat Politècnica de València. Departamento de Ecosistemas Agroforestales - Departament d'Ecosistemes Agroforestals
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Fecha difusión:
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Resumen:
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[EN] Background
Reactive oxygen species (ROS) are normally produced in respiratory and photosynthetic electron chains and their production is enhanced during desiccation/rehydration. Nitric oxide (NO) is a ubiquitous and ...[+]
[EN] Background
Reactive oxygen species (ROS) are normally produced in respiratory and photosynthetic electron chains and their production is enhanced during desiccation/rehydration. Nitric oxide (NO) is a ubiquitous and multifaceted molecule involved in cell signaling and abiotic stress. Lichens are poikilohydrous organisms that can survive continuous cycles of desiccation and rehydration. Although the production of ROS and NO was recently demonstrated during lichen rehydration, the functions of these compounds are unknown. The aim of this study was to analyze the role of NO during rehydration of the lichen Ramalina farinacea (L.) Ach., its isolated photobiont partner Trebouxia sp. and Asterochloris erici (Ahmadjian) Skaloud et Peksa (SAG 32.85 = UTEX 911).
Results
Rehydration of R. farinacea caused the release of ROS and NO evidenced by the fluorescent probes DCFH2-DA and DAN respectively. However, a minimum in lipid peroxidation (MDA) was observed 2 h post-rehydration. The inhibition of NO in lichen thalli with c-PTIO resulted in increases in both ROS production and lipid peroxidation, which now peaked at 3 h, together with decreases in chlorophyll autofluorescence and algal photobleaching upon confocal laser incidence. Trebouxia sp. photobionts generate peaks of NO-endproducts in suspension and show high rates of photobleaching and ROS production under NO inhibition which also caused a significant decrease in photosynthetic activity of A. erici axenic cultures, probably due to the higher levels of photo-oxidative stress.
Conclusions
Mycobiont derived NO has an important role in the regulation of oxidative stress and in the photo-oxidative protection of photobionts in lichen thalli. The results point to the importance of NO in the early stages of lichen rehydration.
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Palabras clave:
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Photosynthetic electron-transport
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II reaction center
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Non-heme iron
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Nitric-oxide
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Desiccation tolerance
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Photosystem-II
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Derechos de uso:
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Reconocimiento (by)
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Fuente:
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BMC Microbiology. (issn:
1471-2180
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DOI:
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10.1186/1471-2180-10-297
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Editorial:
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BioMed Central
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Versión del editor:
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http://dx.doi.org/ 10.1186/1471-2180-10-297
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Código del Proyecto:
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info:eu-repo/grantAgreement/MEC//CGL2006-12917-C02-01/ES/FOTOBIONTES DE LIQUENES: DIVERSIDAD MORFOLOGICA, GENETICA Y FUNCIONAL. RESPUESTAS FRENTE A CONDICIONES ADVERSAS./
info:eu-repo/grantAgreement/GVA//PROMETEO08%2F2008%2F174/
info:eu-repo/grantAgreement/MAEC//A%2F024755%2F09/ES/CARACTERIZACIÓN GENÉTICA, FISIOLÓGICA Y ANATÓMICA DE LOS LÍQUENES BIOINDICADORES DE BOSQUES SUSTENTABLES Y VULNERABLES AL CAMBIO CLIMÁTICO. PARQUE NACIONAL EL CHICO (HIDALGO) Y FSC EN SIERRA DE JUÁREZ (OAXACA), MÉXICO/
info:eu-repo/grantAgreement/MICINN//CGL2009-13429-C02-01/
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Agradecimientos:
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This project was funded by the Spanish Ministry of Education and Science [project numbers CGL2006 12917 C02 0 and CGL2009 13429 C02 01], project Prometeo 2008/1/4 of the Generalitat Valenciana and the project AECID ...[+]
This project was funded by the Spanish Ministry of Education and Science [project numbers CGL2006 12917 C02 0 and CGL2009 13429 C02 01], project Prometeo 2008/1/4 of the Generalitat Valenciana and the project AECID PCI/A/024755/09 of the Spanish Ministry of Foreign Affaires. We are grateful to F. Gasulla, J. Gimeno-Romeu, E. Barreno, (ICBIBE, University of Valencia) and A. Guera (Plant Biology, University of Alcala) for communicating unpublished data, to Dr. R. Catala (CIB, Madrid), Dr. P. D'Ocon (UVEG, Valencia) and Dr. J. Medina (INIA, Madrid) for critical revision of the manuscript, and J. L. Rodriguez Gil for MDA protocol optimization. English revision was done by Wendy Ran.
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Tipo:
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Artículo
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