dc.contributor.author |
Minoia, Sofia
|
es_ES |
dc.contributor.author |
Navarro, B
|
es_ES |
dc.contributor.author |
Delgado Villar, Sonia Guadalupe
|
es_ES |
dc.contributor.author |
Di Serio, Francesco
|
es_ES |
dc.contributor.author |
Flores Pedauye, Ricardo
|
es_ES |
dc.date.accessioned |
2017-07-19T09:06:32Z |
|
dc.date.available |
2017-07-19T09:06:32Z |
|
dc.date.issued |
2015-02-27 |
|
dc.identifier.issn |
0305-1048 |
|
dc.identifier.uri |
http://hdl.handle.net/10251/85435 |
|
dc.description.abstract |
[EN] While biogenesis of viroid RNAs is well-known, how they decay is restricted to data involving host RNA silencing. Here we report an alternative degradation pathway operating on potato spindle tuber viroid (PSTVd), the type species of nuclear-replicating viroids (family Pospiviroidae). Northern-blot hybridizations with full-and partial-length probes revealed a set of PSTVd (+) subgenomic (sg) RNAs in early-infected eggplant, some partially overlapping and reaching levels comparable to those of the genomic circular and linear forms. Part of the PSTVd (+) sgRNAs were also observed in Nicotiana benthamiana (specifically in the nuclei) and tomato, wherein they have been overlooked due to their low accumulation. Primer extensions of representative (+) sgRNAs failed to detect a common 5' terminus, excluding that they could result from aborted transcription initiated at one specific site. Supporting this view, 5'- and 3'-RACE indicated that the (+) sgRNAs have 5'-OH and 3'-P termini most likely generated by RNase-mediated endonucleolytic cleavage of longer precursors. These approaches also unveiled PSTVd (-) sgRNAs with features similar to their (+) counterparts. Our results provide a mechanistic insight on how viroid decay may proceed in vivo during replication, and suggest that synthesis and decay of PSTVd strands might be coupled as in mRNA. |
es_ES |
dc.description.sponsorship |
Ministerio de Economia y Competitividad (MINECO) [BFU2011-28443 to R. F. laboratory]; MINECO [to S.M. and S.D.]. Funding for open access charge: MINECO (BFU2011-28443 to R. F.); Ministero dell'Economia e Finanze Italiano to the CNR (CISIA, Legge 191/2009 to B.N. and F.D.S.). |
en_EN |
dc.language |
Inglés |
es_ES |
dc.publisher |
Oxford University Press (OUP) |
es_ES |
dc.relation |
Ministerio de Economía y Competitividad (MINECO), Spain [BFU2011-28443] |
es_ES |
dc.relation |
Ministero dell'Economia e Finanze Italiano (CISIA) [Legge 191/2009] |
es_ES |
dc.relation.ispartof |
Nucleic Acids Research |
es_ES |
dc.rights |
Reconocimiento - No comercial (by-nc) |
es_ES |
dc.subject |
Nicotiana benthamiana |
es_ES |
dc.subject |
Systemic trafficking |
es_ES |
dc.subject |
hammerhead ribozymes |
es_ES |
dc.subject |
Secondary structure |
es_ES |
dc.subject |
Situ hybridization |
es_ES |
dc.subject |
Host interactions |
es_ES |
dc.subject |
Rolling circle |
es_ES |
dc.subject |
Self cleavage |
es_ES |
dc.subject |
Replication |
es_ES |
dc.subject |
Sequence |
es_ES |
dc.title |
Viroid RNA turnover: characterization of the subgenomic RNAs of potato spindle tuber viroid accumulating in infected tissues provides insights into decay pathways operating in vivo |
es_ES |
dc.type |
Artículo |
es_ES |
dc.identifier.doi |
10.1093/nar/gkv034 |
|
dc.rights.accessRights |
Abierto |
es_ES |
dc.contributor.affiliation |
Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes |
es_ES |
dc.description.bibliographicCitation |
Minoia, S.; Navarro, B.; Delgado Villar, SG.; Di Serio, F.; Flores Pedauye, R. (2015). Viroid RNA turnover: characterization of the subgenomic RNAs of potato spindle tuber viroid accumulating in infected tissues provides insights into decay pathways operating in vivo. Nucleic Acids Research. 43(4):2313-2325. doi:10.1093/nar/gkv034 |
es_ES |
dc.description.accrualMethod |
S |
es_ES |
dc.relation.publisherversion |
http://doi.org/10.1093/nar/gkv034 |
es_ES |
dc.description.upvformatpinicio |
2313 |
es_ES |
dc.description.upvformatpfin |
2325 |
es_ES |
dc.type.version |
info:eu-repo/semantics/publishedVersion |
es_ES |
dc.description.volume |
43 |
es_ES |
dc.description.issue |
4 |
es_ES |
dc.relation.senia |
308391 |
es_ES |
dc.identifier.eissn |
1362-4962 |
|
dc.identifier.pmid |
25662219 |
en_EN |
dc.identifier.pmcid |
PMC4344493 |
|