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Viroid RNA turnover: characterization of the subgenomic RNAs of potato spindle tuber viroid accumulating in infected tissues provides insights into decay pathways operating in vivo

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Viroid RNA turnover: characterization of the subgenomic RNAs of potato spindle tuber viroid accumulating in infected tissues provides insights into decay pathways operating in vivo

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dc.contributor.author Minoia, Sofia es_ES
dc.contributor.author Navarro, B es_ES
dc.contributor.author Delgado Villar, Sonia Guadalupe es_ES
dc.contributor.author Di Serio, Francesco es_ES
dc.contributor.author Flores Pedauye, Ricardo es_ES
dc.date.accessioned 2017-07-19T09:06:32Z
dc.date.available 2017-07-19T09:06:32Z
dc.date.issued 2015-02-27
dc.identifier.issn 0305-1048
dc.identifier.uri http://hdl.handle.net/10251/85435
dc.description.abstract [EN] While biogenesis of viroid RNAs is well-known, how they decay is restricted to data involving host RNA silencing. Here we report an alternative degradation pathway operating on potato spindle tuber viroid (PSTVd), the type species of nuclear-replicating viroids (family Pospiviroidae). Northern-blot hybridizations with full-and partial-length probes revealed a set of PSTVd (+) subgenomic (sg) RNAs in early-infected eggplant, some partially overlapping and reaching levels comparable to those of the genomic circular and linear forms. Part of the PSTVd (+) sgRNAs were also observed in Nicotiana benthamiana (specifically in the nuclei) and tomato, wherein they have been overlooked due to their low accumulation. Primer extensions of representative (+) sgRNAs failed to detect a common 5' terminus, excluding that they could result from aborted transcription initiated at one specific site. Supporting this view, 5'- and 3'-RACE indicated that the (+) sgRNAs have 5'-OH and 3'-P termini most likely generated by RNase-mediated endonucleolytic cleavage of longer precursors. These approaches also unveiled PSTVd (-) sgRNAs with features similar to their (+) counterparts. Our results provide a mechanistic insight on how viroid decay may proceed in vivo during replication, and suggest that synthesis and decay of PSTVd strands might be coupled as in mRNA. es_ES
dc.description.sponsorship Ministerio de Economia y Competitividad (MINECO) [BFU2011-28443 to R. F. laboratory]; MINECO [to S.M. and S.D.]. Funding for open access charge: MINECO (BFU2011-28443 to R. F.); Ministero dell'Economia e Finanze Italiano to the CNR (CISIA, Legge 191/2009 to B.N. and F.D.S.). en_EN
dc.language Inglés es_ES
dc.publisher Oxford University Press (OUP) es_ES
dc.relation Ministerio de Economía y Competitividad (MINECO), Spain [BFU2011-28443] es_ES
dc.relation Ministero dell'Economia e Finanze Italiano (CISIA) [Legge 191/2009] es_ES
dc.relation.ispartof Nucleic Acids Research es_ES
dc.rights Reconocimiento - No comercial (by-nc) es_ES
dc.subject Nicotiana benthamiana es_ES
dc.subject Systemic trafficking es_ES
dc.subject hammerhead ribozymes es_ES
dc.subject Secondary structure es_ES
dc.subject Situ hybridization es_ES
dc.subject Host interactions es_ES
dc.subject Rolling circle es_ES
dc.subject Self cleavage es_ES
dc.subject Replication es_ES
dc.subject Sequence es_ES
dc.title Viroid RNA turnover: characterization of the subgenomic RNAs of potato spindle tuber viroid accumulating in infected tissues provides insights into decay pathways operating in vivo es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1093/nar/gkv034
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Minoia, S.; Navarro, B.; Delgado Villar, SG.; Di Serio, F.; Flores Pedauye, R. (2015). Viroid RNA turnover: characterization of the subgenomic RNAs of potato spindle tuber viroid accumulating in infected tissues provides insights into decay pathways operating in vivo. Nucleic Acids Research. 43(4):2313-2325. doi:10.1093/nar/gkv034 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1093/nar/gkv034 es_ES
dc.description.upvformatpinicio 2313 es_ES
dc.description.upvformatpfin 2325 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 43 es_ES
dc.description.issue 4 es_ES
dc.relation.senia 308391 es_ES
dc.identifier.eissn 1362-4962
dc.identifier.pmid 25662219 en_EN
dc.identifier.pmcid PMC4344493


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