González Martínez, MÁ.; Morais, S.; Puchades, R.; Maquieira Giménez, M.; Abad Fuentes, A.; Montoya Baides, Á. (1997). Development of an automated controlled-pore glass flow-through immunosensor for carbaryl. Analytica Chimica Acta. 347(1):199-205. https://doi.org/10.1016/S0003-2670(97)00003-2
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/85607
Title:
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Development of an automated controlled-pore glass flow-through immunosensor for carbaryl
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Author:
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González Martínez, Miguel Ángel
Morais, Sergi
Puchades, Rosa
Maquieira Giménez, Manuel
Abad Fuentes, Antonio
Montoya Baides, Ángel
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UPV Unit:
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Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials
Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural
Universitat Politècnica de València. Departamento de Química - Departament de Química
Universitat Politècnica de València. Laboratorio Integrado de Bioingeniería - Laboratori Integrat de Bioenginyeria
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Issued date:
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Abstract:
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[EN] The application of controlled-pore glass (CPG) as solid support for immobilization of immunoreagents in order to develop flow-through immunosensors is described. Monoclonal antibodies (MAbs) to carbaryl were site-directed ...[+]
[EN] The application of controlled-pore glass (CPG) as solid support for immobilization of immunoreagents in order to develop flow-through immunosensors is described. Monoclonal antibodies (MAbs) to carbaryl were site-directed immobilized on CPG through covalent attachment of their oxidized carbohydrate moieties to amine groups generated on the surface of silanyzed CPG. The automated immunosensor system is based on the LIB-CNA36 MAb in a direct competitive assay format, with horseradish peroxidase as enzyme label and fluorimetric detection. The dynamic range of the sensor is 0.05-1 mu g l(-1), with a detection limit of 0.029 mu g l(-1), being sensitive enough to be applied to drinking water samples without preconcentration. The immobilized antibody reactor is able to run a whole assay in 20 min, and is reusable for more than 100 of consecutive assay cycles without significant loss of performance. The recognition of 1-naphthol -the main metabolite of carbaryl -and other N-methylcarbamate insecticides are also studied, none of these compounds showing cross-reactivity higher than 7%. A preliminary validation of the immunosensor, carried out by analysing real samples spiked with carbaryl, shows good results for bottled water and for commercial honey diluted with PBS (1 g l(-1)) as the only sample pretreatment.
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Subjects:
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Biosensors
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Controlled-pore glass
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Flow-through immunosensors
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Carbaryl
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Copyrigths:
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Cerrado |
Source:
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Analytica Chimica Acta. (issn:
0003-2670
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DOI:
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10.1016/S0003-2670(97)00003-2
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Publisher:
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Elsevier Masson
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Publisher version:
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http://dx.doi.org/ 10.1016/S0003-2670(97)00003-2
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Conference name:
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2nd Workshop on Biosensors and Biological Techniques in Environmental Analysis
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Conference place:
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Lund, Sweden
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Conference date:
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SEP 11-13, 1996
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Project ID:
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info:eu-repo/grantAgreement/MEC//ALI92-0417/ES/DESAROLLO DE INMUNOENSAYOS BASADOS EN ANTICUERPOS MONOCLONALES PARA EL ANALISIS DE PLAGUICIDAS EN ALIMENTOS. AUTOMATIZACION MEDIANTE ANALISIS POR INYECCION EN FLUJO/
info:eu-repo/grantAgreement/MEC//ALI94-0673/
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Thanks:
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This project was supported by the CICYT project ALI 92-0417 and ALI 94-0673. M.A. González-Martínez acknowledge a grant from Fundación Cajamadrid, Spain, to carry out PhD studies.
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Type:
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Artículo
Comunicación en congreso
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