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Development of an automated controlled-pore glass flow-through immunosensor for carbaryl

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Development of an automated controlled-pore glass flow-through immunosensor for carbaryl

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dc.contributor.author González Martínez, Miguel Ángel es_ES
dc.contributor.author Morais, Sergi es_ES
dc.contributor.author Puchades, Rosa es_ES
dc.contributor.author Maquieira Giménez, Manuel es_ES
dc.contributor.author Abad Fuentes, Antonio es_ES
dc.contributor.author Montoya Baides, Ángel es_ES
dc.date.accessioned 2017-07-21T10:38:50Z
dc.date.available 2017-07-21T10:38:50Z
dc.date.issued 1997-07-30
dc.identifier.issn 0003-2670
dc.identifier.uri http://hdl.handle.net/10251/85607
dc.description.abstract [EN] The application of controlled-pore glass (CPG) as solid support for immobilization of immunoreagents in order to develop flow-through immunosensors is described. Monoclonal antibodies (MAbs) to carbaryl were site-directed immobilized on CPG through covalent attachment of their oxidized carbohydrate moieties to amine groups generated on the surface of silanyzed CPG. The automated immunosensor system is based on the LIB-CNA36 MAb in a direct competitive assay format, with horseradish peroxidase as enzyme label and fluorimetric detection. The dynamic range of the sensor is 0.05-1 mu g l(-1), with a detection limit of 0.029 mu g l(-1), being sensitive enough to be applied to drinking water samples without preconcentration. The immobilized antibody reactor is able to run a whole assay in 20 min, and is reusable for more than 100 of consecutive assay cycles without significant loss of performance. The recognition of 1-naphthol -the main metabolite of carbaryl -and other N-methylcarbamate insecticides are also studied, none of these compounds showing cross-reactivity higher than 7%. A preliminary validation of the immunosensor, carried out by analysing real samples spiked with carbaryl, shows good results for bottled water and for commercial honey diluted with PBS (1 g l(-1)) as the only sample pretreatment. es_ES
dc.description.sponsorship This project was supported by the CICYT project ALI 92-0417 and ALI 94-0673. M.A. González-Martínez acknowledge a grant from Fundación Cajamadrid, Spain, to carry out PhD studies.
dc.language Inglés es_ES
dc.publisher Elsevier Masson es_ES
dc.relation CICYT/ALI 92-0417 es_ES
dc.relation CICYT/ALI 94-0673
dc.relation.ispartof Analytica Chimica Acta es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Biosensors es_ES
dc.subject Controlled-pore glass es_ES
dc.subject Flow-through immunosensors es_ES
dc.subject Carbaryl es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.subject.classification TECNOLOGIA ELECTRONICA es_ES
dc.title Development of an automated controlled-pore glass flow-through immunosensor for carbaryl es_ES
dc.type Artículo es_ES
dc.type Comunicación en congreso
dc.identifier.doi 10.1016/S0003-2670(97)00003-2
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.contributor.affiliation Universitat Politècnica de València. Laboratorio Integrado de Bioingeniería - Laboratori Integrat de Bioenginyeria es_ES
dc.description.bibliographicCitation González Martínez, MÁ.; Morais, S.; Puchades, R.; Maquieira Gimenez, M.; Abad Fuentes, A.; Montoya Baides, Á. (1997). DEVELOPMENT OF AN AUTOMATED CONTROLLED PORE GLASS FLOW-THROUGH IMMUNOSENSOR FOR CARBARYL. Analytica Chimica Acta. 347(1):199-205. doi:10.1016/S0003-2670(97)00003-2 es_ES
dc.description.accrualMethod S es_ES
dc.relation.conferencename 2nd Workshop on Biosensors and Biological Techniques in Environmental Analysis
dc.relation.conferencedate SEP 11-13, 1996
dc.relation.conferenceplace Lund, Sweden
dc.relation.publisherversion http://dx.doi.org/ 10.1016/S0003-2670(97)00003-2 es_ES
dc.description.upvformatpinicio 199 es_ES
dc.description.upvformatpfin 205 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 347 es_ES
dc.description.issue 1 es_ES
dc.relation.senia 14846 es_ES
dc.contributor.funder Comisión Interministerial de Ciencia y Tecnología
dc.contributor.funder Fundación Caja Madrid


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