Forget, J.; Awaja, F.; Gugutkov, D.; Gustavsson, J.; Gallego Ferrer, G.; Coelho-Sampaio, T.; Hochman-Mendez, C.... (2016). Differentiation of Human Mesenchymal Stem Cells Toward Quality Cartilage Using Fibrinogen-Based Nanofibers. Macromolecular Bioscience. 16(9):1348-1359. https://doi.org/10.1002/mabi.201600080
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/88294
Title:
|
Differentiation of Human Mesenchymal Stem Cells Toward Quality Cartilage Using Fibrinogen-Based Nanofibers
|
Author:
|
Forget, Jeremy
Awaja, Firas
Gugutkov, Dencho
Gustavsson, Juhan
Gallego Ferrer, Gloria
Coelho-Sampaio, Tatiana
Hochman-Mendez, Camila
Salmerón Sánchez, Manuel
Altankov, George
|
UPV Unit:
|
Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular
Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials
|
Issued date:
|
|
Abstract:
|
[EN] Mimicking the complex intricacies of the extra cellular matrix including 3D configurations and aligned fibrous structures were traditionally perused for producing cartilage tissue from stem cells. This study shows ...[+]
[EN] Mimicking the complex intricacies of the extra cellular matrix including 3D configurations and aligned fibrous structures were traditionally perused for producing cartilage tissue from stem cells. This study shows that human adipose derived mesenchymal stem cells (hADMSCs) establishes significant chondrogenic differentiation and may generate quality cartilage when cultured on 2D and randomly oriented fibrinogen/poly-lactic acid nanofibers compared to 3D sandwich-like environments. The adhering cells show well-developed focal adhesion complexes and actin cytoskeleton arrangements confirming the proper cellular interaction with either random or aligned nanofibers. However, quantitative reverse transcription-polymerase chain reaction analysis for Collagen 2 and Collagen 10 genes expression confirms favorable chondrogenic response of hADMSCs on random nanofibers and shows substantially higher efficacy of their differentiation in 2D configuration versus 3D constructs. These findings introduce a new direction for cartilage tissue engineering through providing a simple platform for the routine generation of transplantable stem cells derived articular cartilage replacement that might improve joint function.
[-]
|
Subjects:
|
cartilage
,
chondrogenic response
,
collagen
,
FBG/PLA nanofibers
,
mesenchymal stem cells
|
Copyrigths:
|
Reserva de todos los derechos
|
Source:
|
Macromolecular Bioscience. (issn:
1616-5187
) (eissn:
1616-5195
)
|
DOI:
|
10.1002/mabi.201600080
|
Publisher:
|
Wiley
|
Publisher version:
|
http://dx.doi. org/10.1002/mabi.201600080
|
Project ID:
|
info:eu-repo/grantAgreement/EC/FP7/324386/EU
MICINN/MAT 2015-69315-C3 MYOHEAL
|
Thanks:
|
This work was supported by CIBER-BBN Spain (project BIOSURFACES), and the European Commission through the FP7 Industry-Academia Partnerships and Pathways (IAPP) project FIBROGELNET. The valuable support of the project MAT ...[+]
This work was supported by CIBER-BBN Spain (project BIOSURFACES), and the European Commission through the FP7 Industry-Academia Partnerships and Pathways (IAPP) project FIBROGELNET. The valuable support of the project MAT 2015-69315-C3 MYOHEAL, funded by Spanish Ministry of Science and Innovation is also acknowledged.
[-]
|
Type:
|
Artículo
|