- -

Differentiation of Human Mesenchymal Stem Cells Toward Quality Cartilage Using Fibrinogen-Based Nanofibers

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

  • Estadisticas de Uso

Differentiation of Human Mesenchymal Stem Cells Toward Quality Cartilage Using Fibrinogen-Based Nanofibers

Show simple item record

Files in this item

dc.contributor.author Forget, Jeremy es_ES
dc.contributor.author Awaja, Firas es_ES
dc.contributor.author Gugutkov, Dencho es_ES
dc.contributor.author Gustavsson, Juhan es_ES
dc.contributor.author Gallego Ferrer, Gloria es_ES
dc.contributor.author Coelho-Sampaio, Tatiana es_ES
dc.contributor.author Hochman-Mendez, Camila es_ES
dc.contributor.author Salmerón Sánchez, Manuel es_ES
dc.contributor.author Altankov, George es_ES
dc.date.accessioned 2017-09-29T12:01:20Z
dc.date.available 2017-09-29T12:01:20Z
dc.date.issued 2016-09
dc.identifier.issn 1616-5187
dc.identifier.uri http://hdl.handle.net/10251/88294
dc.description.abstract [EN] Mimicking the complex intricacies of the extra cellular matrix including 3D configurations and aligned fibrous structures were traditionally perused for producing cartilage tissue from stem cells. This study shows that human adipose derived mesenchymal stem cells (hADMSCs) establishes significant chondrogenic differentiation and may generate quality cartilage when cultured on 2D and randomly oriented fibrinogen/poly-lactic acid nanofibers compared to 3D sandwich-like environments. The adhering cells show well-developed focal adhesion complexes and actin cytoskeleton arrangements confirming the proper cellular interaction with either random or aligned nanofibers. However, quantitative reverse transcription-polymerase chain reaction analysis for Collagen 2 and Collagen 10 genes expression confirms favorable chondrogenic response of hADMSCs on random nanofibers and shows substantially higher efficacy of their differentiation in 2D configuration versus 3D constructs. These findings introduce a new direction for cartilage tissue engineering through providing a simple platform for the routine generation of transplantable stem cells derived articular cartilage replacement that might improve joint function. es_ES
dc.description.sponsorship This work was supported by CIBER-BBN Spain (project BIOSURFACES), and the European Commission through the FP7 Industry-Academia Partnerships and Pathways (IAPP) project FIBROGELNET. The valuable support of the project MAT 2015-69315-C3 MYOHEAL, funded by Spanish Ministry of Science and Innovation is also acknowledged.
dc.language Inglés es_ES
dc.publisher Wiley es_ES
dc.relation.ispartof Macromolecular Bioscience es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject cartilage es_ES
dc.subject chondrogenic response es_ES
dc.subject collagen es_ES
dc.subject FBG/PLA nanofibers es_ES
dc.subject mesenchymal stem cells es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title Differentiation of Human Mesenchymal Stem Cells Toward Quality Cartilage Using Fibrinogen-Based Nanofibers es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1002/mabi.201600080
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/324386/EU/Network for Development of Soft Nanofibrous Construct for Cellular Therapy of Degenerative Skeletal Disorders/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//MAT2015-69315-C3-1-R/ES/SOPORTES CELULARES BIODEGRADABLES CARGADOS CON IONES BIOACTIVOS PARA REGENERACION MUSCULAR/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//MAT2015-69315-C3-2-R/ES/REMODELACION POR MIOBLASTOS DE LA MATRIZ EXTRACELULAR EN LA INTERFAZ CELULA-BIOMATERIAL/
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingenieros Industriales - Escola Tècnica Superior d'Enginyers Industrials es_ES
dc.description.bibliographicCitation Forget, J.; Awaja, F.; Gugutkov, D.; Gustavsson, J.; Gallego Ferrer, G.; Coelho-Sampaio, T.; Hochman-Mendez, C.... (2016). Differentiation of Human Mesenchymal Stem Cells Toward Quality Cartilage Using Fibrinogen-Based Nanofibers. Macromolecular Bioscience. 16(9):1348-1359. https://doi.org/10.1002/mabi.201600080 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi. org/10.1002/mabi.201600080 es_ES
dc.description.upvformatpinicio 1348 es_ES
dc.description.upvformatpfin 1359 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 16 es_ES
dc.description.issue 9 es_ES
dc.relation.senia 330162 es_ES
dc.identifier.eissn 1616-5195
dc.identifier.pmid 27276166
dc.contributor.funder European Commission
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


This item appears in the following Collection(s)

Show simple item record