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AGO1 CONTROLS INFLORESCENCE ARCHITECTURE POSSIBLY BY REGULATING TFL1 EXPRESSION

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AGO1 CONTROLS INFLORESCENCE ARCHITECTURE POSSIBLY BY REGULATING TFL1 EXPRESSION

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dc.contributor.author Fernández Nohales, Pedro es_ES
dc.contributor.author Domenech Mir, Mª José es_ES
dc.contributor.author Martínez De Alba, Angel Emilio es_ES
dc.contributor.author Micol, JL es_ES
dc.contributor.author Ponce, M.R. es_ES
dc.contributor.author Madueño Albi, Francisco es_ES
dc.date.accessioned 2017-10-20T07:18:57Z
dc.date.available 2017-10-20T07:18:57Z
dc.date.issued 2014 es_ES
dc.identifier.issn 0305-7364 es_ES
dc.identifier.uri http://hdl.handle.net/10251/89668
dc.description.abstract [EN] The TERMINAL FLOWER 1 (TFL1) gene is pivotal in the control of inflorescence architecture in arabidopsis. Thus, tfl1 mutants flower early and have a very short inflorescence phase, while TFL1-overexpressing plants have extended vegetative and inflorescence phases, producing many coflorescences. TFL1 is expressed in the shoot meristems, never in the flowers. In the inflorescence apex, TFL1 keeps the floral genes LEAFY (LFY) and APETALA1 (AP1) restricted to the flower, while LFY and AP1 restrict TFL1 to the inflorescence meristem. In spite of the central role of TFL1 in inflorescence architecture, regulation of its expression is poorly understood. This study aims to expand the understanding of inflorescence development by identifying and studying novel TFL1 regulators. Mutagenesis of an Arabidopsis thaliana line carrying a TFL1::GUS (beta-glucuronidase) reporter construct was used to isolate a mutant with altered TFL1 expression. The mutated gene was identified by positional cloning. Expression of TFL1 and TFL1::GUS was analysed by real-time PCR and histochemical GUS detection. Double-mutant analysis was used to assess the contribution of TFL1 to the inflorescence mutant phenotype. A mutant with both an increased number of coflorescences and high and ectopic TFL1 expression was isolated. Cloning of the mutated gene showed that both phenotypes were caused by a mutation in the ARGONAUTE1 (AGO1) gene, which encodes a key component of the RNA silencing machinery. Analysis of another ago1 allele indicated that the proliferation of coflorescences and ectopic TFL1 expression phenotypes are not allele specific. The increased number of coflorescences is suppressed in ago1 tfl1 double mutants. The results identify AGO1 as a repressor of TFL1 expression. Moreover, they reveal a novel role for AGO1 in inflorescence development, controlling the production of coflorescences. AGO1 seems to play this role through regulating TFL1 expression. es_ES
dc.description.sponsorship We thank Herve Vaucheret for the ago1-26 seeds, Antonio Serrano-Mislata for the pBTG6 construct, and Cristina Ferrandiz for critical reading of the manuscript. The collaboration of the IBMCP staff from the greenhouse, sequencing and microscopy facilities is also acknowledged. This work was supported by grants from the Spanish Ministerio de Ciencia e Innovacion (BIO2009-10876 and CSD2007-00057), the Spanish Ministerio de Economia y Competitividad (BFU2012-38929) and the Generalitat Valenciana (ACOMP2012-101). P.F.N. was supported by a fellowship from the I3P program of CSIC. en_EN
dc.language Inglés es_ES
dc.relation.ispartof Annals of Botany es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Flower development es_ES
dc.subject TERMINAL FLOWER 1 es_ES
dc.subject TFL1 es_ES
dc.subject ARGONAUTE1 es_ES
dc.subject AGO1 es_ES
dc.subject plant architecture es_ES
dc.subject inflorescence architecture es_ES
dc.subject flowering es_ES
dc.subject Arabidopsis thaliana es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title AGO1 CONTROLS INFLORESCENCE ARCHITECTURE POSSIBLY BY REGULATING TFL1 EXPRESSION es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1093/aob/mcu132 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2009-10876/ES/Arquitectura De La Inflorescencia; Genes Que Controlan La Identidad De Los Meristemos Del Tallo/
dc.relation.projectID info:eu-repo/grantAgreement/GVA//ACOM%2FP2012%2F101/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MEC//CSD2007-00057/ES/Función y potencial biotecnológico de los factores de transcripción de las plantas./
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BFU2012-38929/ES/CONTROL DE LA FLORACION: LOS FACTORES DE TRANSCRIPCION VOZ COMO NUEVOS REGULADORES TRANSVERSALES DE LA RED GENETICA./
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Fernández Nohales, P.; Domenech Mir, MJ.; Martínez De Alba, AE.; Micol, J.; Ponce, M.; Madueño Albi, F. (2014). AGO1 CONTROLS INFLORESCENCE ARCHITECTURE POSSIBLY BY REGULATING TFL1 EXPRESSION. Annals of Botany. 114(7):1471-1481. https://doi.org/10.1093/aob/mcu132 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http:dx.doi.org/10.1093/aob/mcu132 es_ES
dc.description.upvformatpinicio 1471 es_ES
dc.description.upvformatpfin 1481 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 114 es_ES
dc.description.issue 7 es_ES
dc.relation.pasarela S\284725 es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación
dc.contributor.funder Ministerio de Economía y Competitividad
dc.contributor.funder Generalitat Valenciana
dc.contributor.funder Consejo Superior de Investigaciones Científicas
dc.contributor.funder Ministerio de Educación y Cultura
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