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In vitro analysis, an accurate tool to estimate dry matter digestibility in rabbits. Intra- and inter- laboratory variability

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In vitro analysis, an accurate tool to estimate dry matter digestibility in rabbits. Intra- and inter- laboratory variability

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dc.contributor.author Carabaño, R.
dc.contributor.author Nicodemus, N.
dc.contributor.author García, J.
dc.contributor.author Xiccato, G.
dc.contributor.author Trocino, A.
dc.contributor.author Pascual Amorós, Juan José
dc.contributor.author Falcão-e-Cunha, L.
dc.contributor.author Maertens, L.
dc.date.accessioned 2011-02-08T13:25:45Z
dc.date.available 2011-02-08T13:25:45Z
dc.date.issued 2008
dc.identifier.issn 1257-5011
dc.identifier.uri http://hdl.handle.net/10251/9508
dc.description.abstract [EN] The aim of the present study was to determine the intra- and inter-laboratory variability of an enzymatic system of in vitro analysis for estimating dry matter (DM) digestibility in rabbits and validating the predicted nutritive value of 4 complete diets and 4 raw materials during three different periods of time. Chemical composition, DM digestibility and digestible energy (diets only) were known. In vitro DM digestibility (DMdinv) of all samples was determined by 4 laboratories (triplicate analysis) at different times with an interval of one month between analyses. DMdinv variability and chemical parameters were measured in terms of repeatability (SR: intra-series variability within each laboratory), reproducibility (SL: intra-series variability among laboratories) and reliability (SF: variability through time within each laboratory). Both the laboratory and sample affected DMdinv values (P<0.001). The period of time also had a significant effect (P=0.002) on mean DMdinv values (67.4, 66.8 and 67.0% for the 1st, 2nd and 3rd month, respectively). Significant laboratory x sample, time x laboratory and time x sample interaction effects were also observed. Repeatability, reproducibility and reliability values for the diets were better than those obtained for the raw materials (by 2.0, 1.9 and 2.4 times, respectively). Repeatability values were also better than the values obtained for reproducibility and reliability (by 2.2 and 3.6 times, respectively). Repeatability and reproducibility values were consistently worse for raw materials than for complete diets (by 1.5, 4, 2.9 and 1.3, 4.3, 2.8 times for SR and SL in period 1, period 2 and period 3, respectively), and were also worse in period 1 with respect to the other two periods (by 2.1 and 2.2 times for SR and SL, respectively). Finally, the in vitro method always showed better coefficients of variation of repeatability (CVR) and reproducibility (CVL) than those of the chemical parameters frequently used as predictors of dietary energy value (acid detergent fibre and crude fibre) (1.73 vs. 2.41 and 3.88 for CVR and 3.24 vs. 3.70 and 5.17 for CVL, respectively). In conclusion, the proposed in vitro methodology showed adequate repeatability and reproducibility, being suitable for predictive purposes. es_ES
dc.description.sponsorship This research was supported by ERAFE project CE-FAIR (3-CT96-1651)
dc.language Inglés es_ES
dc.publisher World Rabbit Science. ICTA. UPV es_ES
dc.relation EC/ERAFE/CE-FAIR (3-CT96-1651)
dc.relation.ispartof World Rabbit Science
dc.rights Reserva de todos los derechos es_ES
dc.subject In vitro dry matter digestibility es_ES
dc.subject Repeatability es_ES
dc.subject Reproducibility es_ES
dc.subject Reliability es_ES
dc.subject Rabbit es_ES
dc.title In vitro analysis, an accurate tool to estimate dry matter digestibility in rabbits. Intra- and inter- laboratory variability es_ES
dc.type Artículo es_ES
dc.date.updated 2011-02-08T13:09:59Z
dc.identifier.doi 10.4995/wrs.2008.614
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Carabaño, R.; Nicodemus, N.; García, J.; Xiccato, G.; Trocino, A.; Pascual Amorós, JJ.; Falcão-E-Cunha, L.... (2008). In vitro analysis, an accurate tool to estimate dry matter digestibility in rabbits. Intra- and inter- laboratory variability. World Rabbit Science. 16(4). doi:10.4995/wrs.2008.614 es_ES
dc.description.accrualMethod SWORD es_ES
dc.relation.publisherversion https://doi.org/10.4995/wrs.2008.614
dc.description.volume 16
dc.description.issue 4
dc.identifier.eissn 1989-8886 es_ES
dc.contributor.funder European Commission


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