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A new genomic library of melon introgression lines in a cantaloupe genetic background for dissecting desirable agronomical traits

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A new genomic library of melon introgression lines in a cantaloupe genetic background for dissecting desirable agronomical traits

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dc.contributor.author Perpiñá Martín, Gorka es_ES
dc.contributor.author Esteras Gómez, Cristina es_ES
dc.contributor.author Gibon, Y. es_ES
dc.contributor.author Monforte Gilabert, Antonio José es_ES
dc.contributor.author Picó Sirvent, María Belén es_ES
dc.date.accessioned 2018-01-18T12:22:57Z
dc.date.available 2018-01-18T12:22:57Z
dc.date.issued 2016 es_ES
dc.identifier.issn 1471-2229 es_ES
dc.identifier.uri http://hdl.handle.net/10251/95102
dc.description.abstract [EN] Background: Genomic libraries of introgression lines (ILs) consist of collections of homozygous lines with a single chromosomal introgression from a donor genotype in a common, usually elite, genetic background, representing the whole donor genome in the full collection. Currently, the only available melon IL collection was generated using Piel de sapo (var. inodorus) as the recurrent background. ILs are not available in genetic backgrounds representing other important market class cultivars, such as the cantalupensis. The recent availability of genomic tools in melon, such as SNP collections and genetic maps, facilitates the development of such mapping populations. Results: We have developed a new genomic library of introgression lines from the Japanese cv. Ginsen Makuwa (var. makuwa) into the French Charentais-type cv. Vedrantais (var. cantalupensis) genetic background. In order to speed up the breeding program, we applied medium-throughput SNP genotyping with Sequenom MassARRAY technology in early backcross generations and High Resolution Melting in the final steps. The phenotyping of the backcross generations and of the final set of 27 ILs (averaging 1.3 introgressions/plant and covering nearly 100 % of the donor genome), in three environments, allowed the detection of stable QTLs for flowering and fruit quality traits, including some that affect fruit size in chromosomes 6 and 11, others that change fruit shape in chromosomes 7 and 11, others that change flesh color in chromosomes 2, 8 and 9, and still others that increase sucrose content and delay climacteric behavior in chromosomes 5 and 10. Conclusions: A new melon IL collection in the Charentais genetic background has been developed. Genomic regions that consistently affect flowering and fruit quality traits have been identified, which demonstrates the suitability of this collection for dissecting complex traits in melon. Additionally, pre-breeding lines with new, commercially interesting phenotypes have been observed, including delayed climacteric ripening associated to higher sucrose levels, which is of great interest for Charentais cultivar breeding. es_ES
dc.description.sponsorship This work has been carried out in the framework of the Programa de Valorización y Recursos Conjuntos de I + D + i de VLC/CAMPUS and has been funded by the Ministerio de Educación, Cultura y Deporte as part of the Programa Campus de Excelencia Internacional. IL generation, genotyping and phenotyping was supported by SAFQIM project, AGL2012-40130-C02-02 of the Spanish Ministry of Economy and Competitivity (MINECO). The authors also wish to thank the MINECO project AGL2014-53398-C2-2-R, co-funded with FEDER funds. es_ES
dc.language Inglés es_ES
dc.publisher Springer (Biomed Central Ltd.) es_ES
dc.relation.ispartof BMC Plant Biology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Melon es_ES
dc.subject Introgression line es_ES
dc.subject Charentais es_ES
dc.subject Fruit quality es_ES
dc.subject Soluble solids concentration es_ES
dc.subject QTLs es_ES
dc.subject.classification GENETICA es_ES
dc.title A new genomic library of melon introgression lines in a cantaloupe genetic background for dissecting desirable agronomical traits es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1186/s12870-016-0842-0 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2012-40130-C02-02/ES/DESCIFRANDO LA BASE GENETICA DE LA MORFOLOGIA DEL FRUTO Y LA DOMESTICACION DE MELON/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2014-53398-C2-2-R/ES/APROXIMACIONES BIOTECNOLOGICAS Y CULTURALES PARA LA MEJORA DE LAS RESISTENCIAS Y EL CONTROL DE ENFERMEDADES EN MELON Y SANDIA/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.description.bibliographicCitation Perpiñá Martín, G.; Esteras Gómez, C.; Gibon, Y.; Monforte Gilabert, AJ.; Picó Sirvent, MB. (2016). A new genomic library of melon introgression lines in a cantaloupe genetic background for dissecting desirable agronomical traits. BMC Plant Biology. 16:1-21. https://doi.org/10.1186/s12870-016-0842-0 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1186/s12870-016-0842-0 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 21 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 16 es_ES
dc.identifier.pmid 27390934 en_EN
dc.identifier.pmcid PMC4938994 en_EN
dc.relation.pasarela S\316358 es_ES
dc.contributor.funder Ministerio de Educación, Cultura y Deporte es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder European Regional Development Fund es_ES
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